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In-vitro preservation method of mouse epididymis

A preservation method and mouse technology, applied in the field of biomedicine, can solve the problems of death, expensive freight, and diseases transmitted by living animals, and achieve the effects of simple operation, saving transportation costs, and prolonging the storage time in vitro

Pending Publication Date: 2021-03-26
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, transporting live mice requires expensive freight, and live animals also have the risk of spreading diseases, and animals may escape or die during transportation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The in vitro preservation method of mouse epididymis in this embodiment comprises the following steps:

[0020] Step 1. Euthanize 3-month-old male mice by cervical dislocation, disinfect with alcohol, take out the caudate epididymis in a biosafety cabinet, remove fat and blot dry blood on filter paper.

[0021] Step 2, transfer the cauda epididymis taken out in step 1 into a 1.5mL centrifuge tube containing 0.2mL Lifor culture medium.

[0022] Step 3, place the centrifuge tube for storing the caudate epididymis treated in step 2 in a 4°C cold chain box for preservation.

[0023] Step 4: The cold-chain box is transported by car for 4 hours to a scientific research institute, where the cold-chain box stays overnight in the scientific research institute until the next day to collect sperm, and stored in a 4°C cold-chain box for 24 hours to maintain sperm vitality and reproductive ability.

Embodiment 2

[0025] The in vitro preservation method of mouse epididymis in this embodiment comprises the following steps:

[0026] Step 1. Euthanize 8-month-old male mice by cervical dislocation, disinfect with alcohol, take out the cauda epididymis in a biosafety cabinet, remove fat and blot dry blood on filter paper.

[0027] Step 2, transfer the cauda epididymis taken out in step 1 into a 1.5mL centrifuge tube containing 0.2mL Lifor culture medium.

[0028] Step 3, place the centrifuge tube for storing the tail of the epididymis treated in step 2 in the refrigerator, and the temperature of the refrigerator is 5°C.

[0029] Step 4: After storing the centrifuge tube in the refrigerator for 72 hours, extract the sperm to maintain the vitality and reproductive ability of the sperm.

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Abstract

The invention relates to an in-vitro preservation method of mouse epididymis, and belongs to the technical field of biomedicine. The method comprises the following steps of: 1) removing fat from a mouse epididymis tail and sucking the epididymis tail on filter paper; (2) transferring the epididymis tail treated in the step (1) into a centrifuge tube filled with a Lifor culture solution; 3) refrigerating and storing the centrifuge tube; and (4) storing the centrifuge tube refrigerated in the step (3) for 72h under the condition that the environment temperature is not higher than 22DEG C to keepthe sperm motility and reproductive capacity . The operation is simple, the in-vitro preservation time of the mouse epididymis can be effectively prolonged, and the transportation cost is saved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an in vitro preservation method of mouse epididymis. Background technique [0002] Mice are the most widely used experimental animals in the field of biomedicine. Using mouse genetic engineering technology, scientists have established hundreds of thousands of genetically engineered mice, many of which have become disease models that can simulate the occurrence of human diseases. These models can be used to deeply study the molecular mechanism of diseases and observe the efficacy of drugs Wait. [0003] For the exchange of genetically engineered mouse strains among various research institutions, the current common practice is to choose to introduce live mice or frozen sperm and embryos. However, transporting live mice requires expensive freight, and live animals also have the risk of spreading diseases, and animals may escape or die during transportation. The introduction o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0252
Inventor 顾美儿杨伟伟桂飞孙筱品
Owner HANGZHOU NORMAL UNIVERSITY
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