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NK (Natural Killer) cell protection transportation liquid

A technology of NK cells and transport liquid, applied in the field of immune cells, can solve the problems of limited wide application and short time, and achieve the effects of low transportation cost, simple operation and high safety

Active Publication Date: 2022-04-15
苏州科贝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, ordinary refrigerators can be used for storage, and the production and transportation costs and operating techniques are basically the same as those stored at room temperature, which can improve the storage capacity and utilization of cells, and expand the scope of cell supply. Short, which limits the wide application of cryopreservation

Method used

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  • NK (Natural Killer) cell protection transportation liquid
  • NK (Natural Killer) cell protection transportation liquid
  • NK (Natural Killer) cell protection transportation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A kind of NK cell protection transport liquid, according to the following volume ratio,

[0045] HEPES buffer 76mL

[0046] 20wt% human albumin 5mL

[0047] 0.5wt% mango extract solution 5mL

[0048] 1wt% glycine solution 5mL

[0049] 5wt% glucose injection 6mL

[0050] 50wt% trehalose solution 3mL

[0051] Mix evenly to obtain the NK cell protection transport solution.

Embodiment 2

[0053] A kind of NK cell protection transport liquid, according to the following volume ratio,

[0054] HEPES buffer 70mL

[0055] 20wt% human albumin 5mL

[0056] 0.5wt% mango extract solution 5mL

[0057] 1wt% glycine solution 5mL

[0058] 5wt% glucose injection 10mL

[0059] 50wt% trehalose solution 5mL

[0060] Mix evenly to obtain the NK cell protection transport solution.

Embodiment 3

[0062] A kind of NK cell protection transport liquid, according to the following volume ratio,

[0063]

[0064] Mix evenly to obtain the NK cell protection transport solution.

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Abstract

The invention relates to the technical field of immune cells, in particular to NK cell protection transport fluid. The NK cell protection transportation liquid comprises the following components: an HEPES buffer solution, a glucose injection, a trehalose solution, human serum albumin, a mango extract and glycine, and has the advantages of being suitable for low-temperature preservation, high in cell activity, long in preservation time, high in safety, low in transportation cost and simple to operate.

Description

technical field [0001] The invention relates to the technical field of immune cells, in particular to an NK cell protection transport solution. Background technique [0002] As the cytotoxic lymphocytes of the body's innate immune system, natural killer cells (NK) play a very important role in the body's immune surveillance, and play an important role in the anti-tumor and anti-virus immune responses. NK cells recognize target cells without MHC restriction, without antigen activation, and without the assistance of antibodies, they can directly recognize and kill target cells non-specifically, such as killing virus-infected cells and tumor cells, and this anti-infection The anti-tumor killing effect is broad-spectrum, and it is an important cell component of the natural immune system. It has the functions of immune surveillance and immune clearance, and is the first barrier of the human defense system. At the same time, it can also produce a series of cytokines, and then reg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 胡向兵姬云
Owner 苏州科贝生物技术有限公司
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