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Normal temperature preservation solution and preservation method of ovary

A room temperature preservation, ovarian technology, applied in the field of ovarian preservation solution, can solve the problems of oocyte waste, oocyte quantity and quality decline, etc., to ensure the quantity and quality, prolong the in vitro preservation time, and improve the effect of embryo development ability.

Inactive Publication Date: 2016-08-31
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once more than 8 hours, the number and quality of oocytes that can be collected will drop significantly, resulting in a great waste of oocytes

Method used

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  • Normal temperature preservation solution and preservation method of ovary

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Effect test

specific Embodiment approach 1

[0016] Specific embodiment one: In this embodiment, the ovary normal temperature preservation solution includes phosphate buffer, glucose and tea polyphenols; The concentration of tea polyphenols was 20 μmol / L.

[0017] Phosphate buffered saline (PBS) consists of 8g sodium chloride, 0.2g potassium chloride, 1.44g disodium hydrogen phosphate, 0.24g potassium dihydrogen phosphate and the rest of distilled water per liter, and its pH value is 7.2.

specific Embodiment approach 2

[0018] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the ovary room temperature preservation solution also includes antibiotics; the antibiotics in the ovary room temperature preservation solution are penicillin and streptomycin, the concentration of penicillin is 0.06g / L, streptomycin The concentration of element is 0.05g / L. Others are the same as the first embodiment.

[0019] In this embodiment, antibiotics are added to avoid the growth of microorganisms and better ensure the quality of the ovary.

specific Embodiment approach 3

[0020] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the ovary storage solution at room temperature also includes fructose; the concentration of fructose in the ovary storage solution at room temperature is 0.12 g / L. Others are the same as the first or second embodiment.

[0021] In this embodiment, fructose is added, and fructose can quickly penetrate cell tissue, inhibit the growth of ovarian microorganisms, and quickly provide energy to ovarian tissue.

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Abstract

The invention relates to ovary storing liquid and an ovary storing method, in particular to ovary constant-temperature storing liquid and an ovary constant-temperature storing method. The problems that at present, constant-temperature ovary storing time is over short, and the quantity and the quality of oocytes are affected are solved. The ovary constant-temperature storing liquid comprises phosphate buffer, glucose and tea polyphenols. The ovary constant-temperature storing method comprises the following steps of (1) removing adipose tissues surrounding an ovary and then rinsing the ovary by using normal saline; and (2) placing the ovary which is cleaned in the step (1) in the ovary constant-temperature storing liquid. The ovary constant-temperature storing liquid has the advantages of high efficiency, low cost, high convenience and zero toxicity on cells. The ovary constant-temperature storing method is simple and is easy to operate; other refrigerating equipment is not required; and the ovary constant-temperature storing liquid and the ovary constant-temperature storing method are particularly suitable for collecting ovaries of wild animals in fields; and the collected ovaries of the animals can be conveyed at constant temperature by a long distance.

Description

technical field [0001] The invention relates to an ovary preservation solution and an ovary preservation method. Background technique [0002] With the in-depth research of reproductive biotechnology (such as embryo engineering technologies such as nuclear transfer and transgenic), as well as the development of embryo production in vitro and commercialization of embryo transfer, how to obtain a larger number of high-quality oocytes has become a top priority. The collection and transportation of ovaries generally takes a long time. For example, when wild animal ovaries are obtained from the wild, female animal germ cells are transported between cities or internationally, and livestock ovaries are obtained from slaughterhouses, etc., the ovaries will be placed outside the body for a long time, resulting in the loss of oocytes. Decreased cell development. [0003] Although the in vitro production of embryos by cryopreservation of ovaries has been successful, the maturation rat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 曹新燕许保增薛海龙李晓霞王世勇刁云飞魏海军赵伟刚赵蒙徐超杨义峰鞠妍宋兴超
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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