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Pseudomonas Z-12 and application thereof in removing heavy metal cadmium

A technology of Pseudomonas and Z-12, applied in the field of microbial treatment of heavy metals in soil, can solve the problems of unclear mechanism of action, difficult to analyze the mechanism of cadmium transformation, complex soil composition and structure, etc., to solve the problem of heavy metal cadmium pollution in soil, The effect of short cultivation period and low cost

Active Publication Date: 2021-03-19
HUNAN AGRI BIOTECH RES CENT +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the mechanism of microbial removal of iron-manganese-bound cadmium is still unclear, mainly because the soil composition and structure are extremely complex
The heavy metal cadmium is very small in the soil, and the change of the iron-manganese bound cadmium is even more negligible. It is difficult to analyze the transformation mechanism of the cadmium form in the soil with the current technical means.

Method used

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  • Pseudomonas Z-12 and application thereof in removing heavy metal cadmium
  • Pseudomonas Z-12 and application thereof in removing heavy metal cadmium
  • Pseudomonas Z-12 and application thereof in removing heavy metal cadmium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Isolation, purification and identification of bacterial strain (Pseudomonas sp.) Z-12

[0040] Single bacteria isolation: The strain was isolated and purified from a soil sample collected from a heavily cadmium-contaminated paddy field in Xiangtan City, Hunan Province. The details are as follows: 100 mL of deionized water, 2 g of glucose, 0.2 g of yeast powder, 0.1 g of dipotassium hydrogen phosphate and 0.05 g of magnesium sulfate heptahydrate were added to a 250 mL Erlenmeyer flask, and sterilized at 115° C. for 30 minutes. Then add 5g of cadmium-contaminated soil sample, enrich and cultivate the microorganisms in the soil at 30°C and 180rpm; prepare 200mL of the above medium, add 2% (w / v) of agar powder and 0.005% (w / v) of bromine Cresyl blue indicator, sterilized at 115°C for 30 minutes, poured onto the plate, pipetted 100 microliters of microbial suspension onto the solid medium for coating, cultured at 30°C for 24 hours, selected yellow monobacteria for...

Embodiment 2

[0067] Embodiment 2: the optimization experiment of bacterial strain (Pseudomonas sp.) Z-12 growth condition

[0068] Determination of the optimum pH value for growth: Prepare the above-mentioned medium, divide it into 250mL Erlenmeyer flasks with 100mL each, set up 6 pH value gradients between pH 4.0 and 9.0, and repeat 3 times each, using hydrochloric acid and hydrogen oxidation The sodium solution adjusts the pH of the medium. After sterilizing at 115°C for 30 minutes, inoculate 1mL of bacterial solution, incubate at 30°C and 175rpm, take samples every 4 hours, and detect the OD value at a wavelength of 600nm with a microplate reader.

[0069] Determination of the optimum growth temperature: Prepare the above-mentioned culture medium, divide it into 250mL Erlenmeyer flasks by 100mL each, sterilize at 115°C for 30 minutes, inoculate 1mL of bacterial liquid, and inoculate them at 25°C, 30°C, and 35°C respectively. , 40°C, 45°C, and cultured at 175rpm, with 3 replicates for e...

Embodiment 3

[0075] Embodiment 3: Bacterial strain (Pseudomonas sp.) Z-12 is to cadmium polluted soil total cadmium and effective cadmium removal effect

[0076] The soil samples were all collected from a heavily cadmium-polluted paddy field in Xiangtan City, Hunan Province: take 0-15 cm of plow layer soil, remove plant residues, mix thoroughly, air-dry at room temperature, and grind through a 100-mesh sieve for later use. The experiment was carried out with a solid-liquid ratio of 1:10 (w / v), and an experimental group (Treatment) and a control group (CK) were set up in the experiment, and the bacterial concentration of the experimental group was 8×10 8 cells / mL of the bacterial solution, the control group was added deionized water with the same pH value (pH4.25) as the bacterial solution, and leached under the same conditions, with 3 replicates in each group.

[0077] Weigh 10g of the above soil sample into a 250mL Erlenmeyer flask, and sterilize it at 121°C for 15 minutes. After the samp...

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Abstract

The invention discloses pseudomonas Z-12 and application thereof in removing heavy metal cadmium. The preservation number of the pseudomonas is CCTCC No: M2020785. Manganese dioxide / hematite-cadmium compound is adopted to simulate ferro-manganese combined cadmium in soil, and the desorption efficiency of the pseudomonas on the manganese dioxide / hematite-cadmium compound reaches 81% to the maximum.The strain is used for leaching cadmium-polluted soil, the removal rates of weak acid cadmium and the ferro-manganese combined cadmium are 70% to 80% and 80% to 90% respectively, and the removal rates of total cadmium and effective cadmium are both 75% to 85%. The strain has a good biotransformation effect on the weak acid cadmium and the ferro-manganese combined cadmium in the soil, and has great application prospects in efficient treatment of the soil cadmium pollution problem by microorganisms.

Description

technical field [0001] The invention belongs to the technical field of soil heavy metal treatment by microorganisms, and specifically relates to a strain of pseudomonas Z-12 and its application in efficiently leaching heavy metal cadmium in soil, especially cadmium in weak acid state and iron-manganese bonded state. Background technique [0002] With the development of industry, cadmium pollution in paddy fields has become increasingly serious and has become an environmental problem that needs to be solved urgently. Traditional soil remediation technologies, such as physical remediation and chemical remediation technologies, are often restricted by problems such as high cost and easy generation of secondary pollutants. Microorganisms are characterized by their species diversity, functional diversity, easy cultivation, and fast growth and other characteristics have attracted widespread attention, and the use of microorganisms to control soil cadmium pollution is considered to...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/38
CPCC12N1/20B09C1/10Y02P10/20
Inventor 蒋慧丹刘学端柏连阳刘宏伟朱萍邓燕郝晓东江卢华
Owner HUNAN AGRI BIOTECH RES CENT
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