Separation chip, separation device and separation method

Abstract

The invention provides a separation chip. The separation chip comprises a sample pool, a first chamber, a second chamber and power supplies. The sample pool comprises first filter membranes and secondfilter membranes, and the first filter membranes are opposite to the second filter membranes. The first chamber is connected to the sample pool through the first filter membranes, the first chamber comprises a first outer wall, the first outer wall is arranged on one side, far away from the second filter membranes, of the first filter membranes, and the first chamber is connected to the outside.The second chamber is connected to the sample pool through the second filter membranes, the second chamber comprises a second outer wall, the second outer wall is arranged on one side, away from the first filter membranes, of the second filter membranes, and the second chamber is connected to the outside. The power supplies are used for providing an electric field applied between the first outer wall and the second outer wall. The separation chip provided by the invention can realize continuous separation, the separation efficiency is improved, and the cost is reduced. The invention also provides a separation device and a separation method for separating target particles in a to-be-detected sample.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a separation chip, a separation device and a separation method for separating target particles in a sample to be detected. Background technique [0002] Existing extracellular vesicle isolation methods mainly include methods such as ultracentrifugation, polymer precipitation, immunoprecipitation, and size exclusion. [0003] However, the use of ultracentrifugation to separate extracellular vesicles is costly and time-consuming, and the large centrifugal force may affect the integrity of extracellular vesicles; the polymer precipitation method requires the introduction of polymers, which is not conducive to downstream proteins Omics detection and application and detection of gene amplification; the cost of isolation of extracellular vesicles by immunoprecipitation is high, and only extracellular vesicles of specific components can be isolated; the difference in the size exclusion meth...

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Problems solved by technology

[0003] However, the use of ultracentrifugation to separate extracellular vesicles is costly and time-consuming, and the large centrifugal force may affect the integrity of extracellular vesicles; the polymer precipitation method requires the introduction of polymers, which is not conducive to downstream proteins Omics detection and application and detection of gene amplification; the cost of isolation of extracellular vesicles by immunoprecipitation is high, and only extracellular vesicles of specific components can be isolated; the difference in the size exclusion method of separating exosome samples will affect the Collection time of exovesicles, poor reproducibility

[0004] In addition, there is also a filter membrane separation method that uses a filter membrane to separate samples. However, during the separation process of the traditional filter membrane separation method, the filter membrane is easily blocked, the use efficiency of the filter membrane is low, the replacement is frequent, and the sample separation is not continuous. damage to the filter membrane

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