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Melanoidin in prepared rehmannia roots and application thereof

A technology of Rehmannia glutinosa and melanoidin, which is applied in the field of chemical medicine and can solve problems to be studied and so on.

Active Publication Date: 2021-03-19
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the large polarity and molecular weight of melanoidin substances, it is easy to co-precipitate with polysaccharides and other substances in the process of water extraction and alcohol precipitation of rehmannia glutinosa. Therefore, a large amount of melanoidinoid substances may be mixed in the crude polysaccharide components. The material basis of the effect of yellow tonifying blood needs to be studied

Method used

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  • Melanoidin in prepared rehmannia roots and application thereof
  • Melanoidin in prepared rehmannia roots and application thereof
  • Melanoidin in prepared rehmannia roots and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Extraction, separation and chemical characterization of melanoidins from Rehmannia glutinosa

[0038] 1. Experimental instruments and reagents

[0039] 1260infitiny high-performance liquid chromatography (Agilent Technologies Co., Ltd.); Alltech 3300ELSD detector (Grace, USA); DAD detector (Agilent Technologies, Ltd.); elemental analyzer (elementar, Germany); Tensor27 Fourier transform infrared spectrometer (Germany Bruker Company); ultrapure water treatment system (Millipore, USA); ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); rotary evaporator (Gongyi Yingyu Hi-Tech Instrument Factory)

[0040] Fresh rehmanniae is collected from Wuzhi County, Jiaozuo City, Henan Province. It is identified as the fresh root tuber of Rehmanniae glutinosa (Gaertn.) DC. by Professor Yu Boyang of China Pharmaceutical University. Rehmannia glutinosa is prepared by "nine steaming and nine drying" of rice wine, that is, raw rehmannia glutinosa is mixed with 30% rice wine, ste...

Embodiment 2

[0060] Study on the Blood Enriching Effect of Rehmannia glutinosa melanoidin components

[0061] 1. Purpose of the experiment

[0062] To observe whether rehmannia melanoidin can promote bone marrow hematopoiesis.

[0063] 2. Experimental animals

[0064] Clean-grade male ICR mice, weighing 18-22 g, were purchased from the Medical Comparison Center of Yangzhou University, license number: SCXK (Su) 2017-0007. The animals were reared in an environment with a temperature of 24±1°C and a humidity of 65%, in an independent environment with alternating day and night, well-ventilated, and given free access to food and water. Adapt to the experimental environment before the start of the experiment, eliminate the interference of environmental factors on the experiment, and all operations and handling of experimental animals complied with the requirements of the Animal Ethics Committee of China Pharmaceutical University.

[0065] 3. Experimental drugs

[0066] "Nine Steams and Nine ...

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Abstract

The invention relates to a preparation method and application of melanoidin in prepared rehmannia roots. The method comprises the following steps: by using the prepared rehmannia roots as a raw material, carrying out reflux extraction with pure water, carrying out precipitation with 80% ethanol, removing a supernatant, dissolving the precipitate, performing adsorption with D202 resin at 60 DEG C for 6 h, carrying out eluting with diluted hydrochloric acid, and performing dialysis to obtain the melanoidin in the prepared rehmannia roots. Pharmacodynamic experiments find that the melanoidin in the prepared rehmannia roots has the function of promoting bone marrow hematopoietic function recovery, and can be used for treating diseases related to hematopoietic function loss, including chronic aplastic anemia, hemorrhagic anemia, bone marrow suppression after chemotherapy and the like.

Description

technical field [0001] The invention belongs to the field of chemical medicine and relates to the preparation and medical application of rehmannia glutinosa melanoidin. Background technique [0002] Rehmanniae glutinosa (Gaertn.) DC. is steamed and processed from the rhizome of Rehmanniae glutinosa (Gaertn.) DC. After processing, the properties of Rehmanniae glutinosa (Gaertn.) DC. have been processed. effect. Modern clinical research has also confirmed that Rehmannia glutinosa and its preparations can improve chronic aplastic anemia and other diseases related to hematopoietic dysfunction. Rehmannia glutinosa contains monosaccharides, oligosaccharides, polysaccharides, phenylethanol glycosides, furan aldehyde derivatives and other chemical components, among which crude polysaccharide components are considered to be the main material basis for the blood-enriching effect of rehmannia glutinosa. Due to the limitations of the separation method and efficiency of crude polysacch...

Claims

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Application Information

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IPC IPC(8): A61K36/804A61P7/06
CPCA61K36/804A61P7/06A61K2236/331A61K2236/55A61K2236/51
Inventor 余伯阳戚进龚普阳
Owner CHINA PHARM UNIV
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