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Production method of high-quality phellinus igniarius strain

A production method and strain technology, applied in the field of production of high-quality Phellinus strains, can solve the problems of uneven nutrient absorption, difficulty in uniform distribution of strains, reduction of production efficiency of Phellinus strains and product quality, etc.

Inactive Publication Date: 2021-03-19
GUIZHOU CROP VARIETIES RESOURCE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned patent discloses the culture method of Phellinus sanghuang (Inonotus sanghuang) bacterial classification, has obtained certain effect in cultivating the Phellinus sanghuang bacterial classification, but generally has the defects of slow growth rate and insufficient quality stability of the bacterial classification, an important reason It is difficult for the strains to distribute evenly in the culture medium, and their absorption of nutrients in the culture medium is not uniform, and they will be partially ripened by the culture medium, making them age or even die, which obviously reduces the production efficiency and Product Quality Affects the Medicinal Value of Phellinus

Method used

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  • Production method of high-quality phellinus igniarius strain
  • Production method of high-quality phellinus igniarius strain
  • Production method of high-quality phellinus igniarius strain

Examples

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Effect test

Embodiment 1

[0069] A kind of production method of high-quality Phellinus phyllosa strain, comprises the following steps:

[0070] (1) Mother species culture: Take the Inonotus sanghuangSheng H.Wu, T.hatt&Y.C.Dai strain isolated from the parasitic Phellinus fruiting bodies of wild mulberry trees, and inoculate the strain on the control medium for activation until the colony diameter reaches 3 ~5cm, and then use a puncher to punch a bacterium cake with a diameter of 5mm on the plate, inoculate it on the mother seed medium, and culture it at a constant temperature of 25-26°C in the dark for 7 days to obtain the mother seed;

[0071] (2) Original seed culture: combine the female seed on the original seed culture medium, inoculate about 10 bags of the female seed in each tube, and culture in the dark at 26°C for 20 days to obtain the original seed;

[0072] (3) Cultivation of cultivars: Inoculate the original seeds on the cultivar culture medium, inoculate about 50 bags of cultivars per bag of...

Embodiment 2

[0082] A kind of production method of high-quality Phellinus phyllosa strain, comprises the following steps:

[0083] (1) Mother species culture: Take the Inonotus sanghuangSheng H.Wu, T.hatt&Y.C.Dai strain isolated from the parasitic Phellinus fruiting bodies of wild mulberry trees, and inoculate the strain on the control medium for activation until the colony diameter reaches 3 ~5cm, and then use a puncher to punch a bacterium cake with a diameter of 5mm on the plate, inoculate it on the medium of the mother seed, and cultivate it at a constant temperature of 25-26°C in the dark for 8 days to obtain the mother seed;

[0084] (2) Original seed culture: combine the female seed on the original seed culture medium, inoculate about 10 bags of the female seed in each tube, and culture in the dark at 26°C for 22 days to obtain the original seed;

[0085] (3) Cultivation of cultivars: Inoculate the original seeds on the cultivar culture medium, inoculate about 50 bags of cultivars p...

Embodiment 3

[0095] A kind of production method of high-quality Phellinus phyllosa strain, comprises the following steps:

[0096] (1) Mother species culture: Take the Inonotus sanghuangSheng H.Wu, T.hatt&Y.C.Dai strain isolated from the parasitic Phellinus fruiting bodies of wild mulberry trees, and inoculate the strain on the control medium for activation until the colony diameter reaches 3 ~5cm, and then use a puncher to punch a bacterium cake with a diameter of 5mm on the plate, inoculate it on the medium of the mother seed, and cultivate it at a constant temperature of 25-26°C in the dark for 10 days to obtain the mother seed;

[0097] (2) Original seed culture: combine the female seed on the original seed culture medium, inoculate about 10 bags of the female seed in each tube, and culture in the dark at 26°C for 20 days to obtain the original seed;

[0098] (3) Cultivation of cultivars: Inoculate the original seeds on the cultivar culture medium, inoculate about 50 bags of cultivars ...

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Abstract

The invention belongs to the technical field of edible and medicinal fungus production, and particularly relates to a production method of a high-quality phellinus igniarius strain. The method comprises mother strain culture, stock culture and culture in cultivation. A mother strain culture medium is prepared from the following raw materials in parts by weight of 80-120 parts of mulberry sawdust,80-120 parts of potatoes, 15-25 parts of glucose, 8-12 parts of agar powder and 800-1200 parts of water; a stock culture medium and a cultivar culture medium are prepared from the following raw materials in percentage by weight of 72.5-82.5% of mulberry sawdust, 15-25% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate; and the water content of the culture media is 60-70%. The phellinus linteus strain cultured by the method is stable in quality and high in growth speed; after the strain is inoculated into a mulberry sawdust cultivation material for about 45 days, primordia begin to form, the cultivation period is shortened, strain production is carried out on the mulberry sawdust, and a strain adaptive cultivation method is provided for mulberry planting; and the operation process is simple and easy to implement, convenient to control and suitable for large-scale cultivation of phellinus linteus strains.

Description

technical field [0001] The invention belongs to the technical field of edible and medicinal fungus production, in particular to a production method of high-quality Phellinus fungi. Background technique [0002] Phellinus, usually grows on Morus (Morus L) plants in the south-central region of my country, and its fruiting body is yellowish brown, hence the name Phellinus. It is a perennial precious medicinal fungus developed in recent years, and it is one of the most promising medicinal fungi in my country; it is a member of Polyporaceae, Phellinus and Inontus Generic term for fungi. [0003] Phellinus fruiting bodies are used as medicine, with a slightly bitter taste, and are often used to treat symptoms such as bloody stranguria, prolapse of the anus, diarrhea, leukorrhea, amenorrhea, diarrhea due to spleen deficiency, accumulation of abdominal mass, and addiction to drinking. Modern studies have shown that the water extract of Phellinus Phellinus has an inhibition rate of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20A01G18/40
CPCA01G18/20A01G18/40
Inventor 杨通静胡腾文王沁龚光禄
Owner GUIZHOU CROP VARIETIES RESOURCE INST
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