Mirror image protein information storage and coding technology

An information storage and mirroring technology, applied in the field of bioinformatics, can solve the problems of difficult DNA chemical preparation, inability to realize information preservation, poor biological stability, etc., and achieve simple and convenient information writing, high tolerance to storage conditions, and easy access Effect

Active Publication Date: 2021-03-12
TSINGHUA UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

DNA storage and encoding technology often encounters difficulties in DNA chemical preparation, but the use of DNA template amplification is prone to replication errors and information loss
More importantly, biomolecules such as DNA and proteins based on natural nucleotides or amino acids have poor biological stability, are susceptible to microbial contamination and degrade and disappear over time, and cannot achieve information under specific environmental conditions such as long-term and natural conditions. save
In addition, biological information molecules such as DNA and protein are also easily contaminated by DNA and protein from natural sources.

Method used

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  • Mirror image protein information storage and coding technology
  • Mirror image protein information storage and coding technology
  • Mirror image protein information storage and coding technology

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Mirror protein fragment preparation and sequencing

[0040] 1.1. Preparation of mirror image protein fragments (coding information writing)

[0041] Using the Fmoc solid-phase peptide synthesis method, prepare the following mirror image peptide fragments, refer to figure 1 (a):

[0042] A1 mirror image peptide fragment:

[0043] h 2 N-fGhvwekapGshhwGk(Biotin)-CONH 2 (wherein, k(Biotin) means biotinylated lysine). The specific operation steps are as follows:

[0044] First, add Rink Amide-AM resin to the peptide synthesis tube, use dimethylformamide (DMF) / dichloromethane (DCM) (volume ratio 1:1) to swell, and add benzotriazole-N, N,N',N'-Tetramethyluronium hexafluorophosphate (HBTU, 3.8 equivalents) / Hydroxybenzotriazole (HOBt, 4 equivalents) / Diisopropylethylamine (DIEA, 8 equivalents ) / Fmoc-protected target peptide C-terminal first amino acid (4 times equivalent) was dissolved in DMF, added to the peptide synthesis tube containing resin to react for 2 hours, the r...

Embodiment 2

[0061] Mirror protein encoding library for storing and encoding Chinese characters, see the operation process Figure 7 and 8 .

[0062] 2.1, "Chinese" hexadecimal encoding rules based on mirror protein (information encoding)

[0063] Using the mirror protein coding system, based on the hexadecimal national standard code, it is used for information storage and coding of Chinese words. As shown in Table 1, taking "Tsinghua University", "China University of Science and Technology", "Shanghai University" and "mirror protein" as examples, the present invention defines a mirror protein coding rule, as shown in Table 1, All characters in hexadecimal corresponding to unique d-amino acid coding sequences are listed. This artificially designed encoding rule can further increase the privacy and security of the encoded information. According to the rules in Table 1, the mirror protein coding sequences corresponding to the obtained Chinese information such as "Tsinghua University", "C...

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Abstract

The invention discloses a method for carrying out information storage and encoding by utilizing mirror image protein. The method comprises the following steps: step 1, encoding a computer file; 2, converting the coded sequence into a mirror image protein sequence represented by D-amino acid and glycine; 3, segmenting the mirror image protein sequence, and adding a serial number information sequence, a verification information sequence and an information sequence capable of realizing random data access to two ends of each obtained mirror image protein fragment sequence; and 4, preparing the mirror image protein fragments into a mirror image protein information storage material. Different from the traditional silicon-based storage mode, the method provided by the technology utilizes the coding mirror image protein containing specific information, and has the advantages of large information storage density, long storage time, easy acquisition of a storage medium, unlimited storage and coding medium shape, high storage condition tolerance, no maintenance and the like; therefore, the technology can be used for storing and encoding information with high information density, long storagetime and strong plasticity, and has a wide application prospect in the aspects of mass information storage, universe encoding and the like.

Description

technical field [0001] The invention relates to the field of biological information. Specifically, the present invention relates to Mirror protein information storage and encoding technology. Background technique [0002] The storage and encoding of information enables the inheritance and dissemination of human social civilization. The ancient Chinese ancestors used the media preserved to this day to record their works and art, making the splendid Chinese civilization spread through the ages. For example, oracle bone inscriptions stored and encoded on tortoise shells document the late Shang civilization from 1200 to 1050 BC. Up to now, the information age marked by computers (including personal smart terminals, Internet of Things and genetic databases, etc.) has produced huge amounts of information, and the amount of information storage has shown an exponential growth trend. The development of human civilization has entered the digital world. [0003] The demand for digit...

Claims

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Application Information

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IPC IPC(8): G16B15/20G16B30/10G16B50/00C07K1/04C07K1/06G01N33/68
CPCC07K1/04C07K1/06G01N33/6818G16B15/20G16B30/10G16B50/00
Inventor 刘磊郑基深胡宏岗田长麟梁军石威巍李影
Owner TSINGHUA UNIV
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