A plant Bacillus gbw-f008 producing indole acetic acid and its application
A technology of GBW-F008 and Bacillus, applied in the field of microorganisms, can solve problems such as soil and plant hazards, achieve the effects of increasing average fruit weight, strong in-situ growth-promoting effect, and increasing content
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Embodiment 1
[0078] Example 1: Isolation, screening and identification of Bacillus GBW-F008 in plants
[0079] 1. Isolation and screening of Bacillus GBW-F008 in plants
[0080] Roots of crops were taken from perennial vegetable greenhouses in Hebei Langfang, Shandong Jinan, Tai'an, Laiyang, Zibo, Liaocheng, and Shouguang, put them in medical test sample bags, stored in a refrigerator at 4°C, and separated in time. The samples involved loofah, muskmelon, and pointed The roots of 56 kinds of crops such as peppers, colorful peppers, tomatoes, cucumbers, etc. Take 2g of the sample, put it into 18mL sterile water, shake and extract at 30°C and 200rpm / min for 30min, put the sample solution in a water bath at 75°C for 20min, and then carry out gradient dilution. Take 100μl of each gradient dilution solution and spread it on the NA plate. The coated plate was cultured upside down at 37°C for 48 hours, single colonies of different shapes were picked out with sterilized bamboo sticks, transferred ...
Embodiment 2
[0087] Example 2: Growth and physiological and biochemical characteristics of Bacillus GBW-F008 in plants
[0088] 1. Physiological and biochemical characteristics of GBW-F008
[0089] After culturing Bacillus intraplanta GBW-F008 in the improved NB medium, according to the physiological and biochemical detection methods in the "Bergery Bacterial Identification Manual" and "Common Bacterial System Identification Manual", the physiological and biochemical characteristics of the bacteria were measured, and the results were as follows: Table 1 shows. Bacillus intraplanta GBW-F008 can grow and reproduce in the temperature range of 20°C to 42°C, but its most suitable growth temperature is 37°C, and it can grow normally in the modified NB medium with pH 4.5 to 9.6, and the optimum growth pH The range is 6.5-7.5, the bacteria can tolerate up to 10% NaCl, can produce urease, casein, catalase and oxidase reaction are positive, can use citrate; GBW-F008 can also use xylose, inulin , g...
Embodiment 3
[0094] Embodiment 3: Comparison of the ability of Bacillus GBW-F008 to produce indoleacetic acid under different conditions
[0095] 1. Comparison of quantitative determination methods for IAA produced by GBW-F008
[0096] The standard product of indole acetic acid IAA was purchased from Sigma, with a molecular weight of 175.18 g / moL and a chemical formula of C 10 h 9 NO 2 .
[0097] Using absolute ethanol as a solvent, prepare standard products with IAA concentrations of 10, 20, 30, 40, and 50 mg / L, take 100 μl of IAA standard solution, and add 100 μl of Salkowski colorimetric solution in equal volume. A microplate reader was used to measure the OD540nm absorbance values after 0 min and 30 min of dark reaction respectively. Take the OD difference between the two as the abscissa and the IAA concentration as the ordinate to draw a colorimetric standard curve ( Figure 4 ), the standard curve has good linearity, R 2 = 0.9999.
[0098] Using methanol as a solvent, prepar...
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