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Artificial insemination method for fertilized sebastes schlegeli in camping body

A fish and sperm technology, applied in fish farming, medical science, climate change adaptation, etc., can solve the problems of high stress, easy miscarriage, low mating rate, low fertilization and litter rate, etc., to overcome source instability, The effect of simple operation and low cost

Pending Publication Date: 2021-03-12
山东省海洋资源与环境研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seriously hindered the development of artificial breeding
At present, there are two main methods of artificial breeding of flat scorpionfish. One is to harvest wild broodstock in sea areas or raise them in cages for indoor artificial breeding during the breeding season. The source of broodstock is unstable, transportation is difficult, and stress is great. Problems such as easy abortion and high mortality rate of larvae; there are also disadvantages such as destroying wild natural resources and biodiversity
The second is to adopt the method of indoor mating and breeding of broodstock. If the mating environment is not properly controlled, there will be problems such as low mating rate and low fertilization and litter rate.
Solve the problems of natural mating rate, low fertilization rate or factory mating rate and litter rate caused by changes in sea area temperature and other conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment relates to a kind of artificial insemination method of the internal fertilization fish fish's flat scorpionfish, and the specific steps are as follows:

[0031] Step 1, the acquisition of the sperm dilution solution of the male scorpionfish male broodstock:

[0032] From November 8th to 10th, 2017, at Yantai Taihua Ocean Technology Co., Ltd., 4 sexually mature male broodstock of Scorpius chrysalis over 3 years old were selected in industrialized farming. The male fish were dissected and inserted into the bladder with a 2 mL syringe needle Aspirate urine in a centrifuge tube and store it in a centrifuge tube as a sperm diluent for later use; obtain the testis, weigh it and put it in a petri dish, then quickly add the diluent according to the ratio of testis weight: diluent volume 1:1~1:1.5, Cut the testis with scissors or put it in a container to grind the testis, filter with a 300-mesh filter (sieve silk) to remove tissue fragments, filter twice, ...

Embodiment 2

[0042] Present embodiment relates to a kind of artificial insemination method of the internal fertilization fish fish's flat scorpionfish,

[0043] In this embodiment, except that the broodstock batches are different, other artificial insemination methods are basically the same as in Embodiment 1.

[0044] From November 15th to 20th, 2018, the artificial insemination experiment of the above-mentioned flat scorpionfish was carried out, and 12 anatomically mature male fish were selected, and 120 female broodstock were artificially inseminated.

[0045] On April 25, 2019, the female broodstock began to give birth one after another. The number of female broodstock capable of giving birth was 112, and the success rate of insemination was 93.3% (the success rate of insemination = the number of female broodstock that can give birth / the number of artificially inseminated female fish × 100 % = 112 / 120×100% =93.3%), the range of litter size per female is 7.2×10 4 ~28.3 × 10 4 tail....

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Abstract

The invention provides an artificial insemination method for fertilized sebastes schlegeli in a camping body. The method comprises the steps of preparation of sebastes schlegeli sperm diluent, determination of sperm motility, artificial insemination of female parent fishes, culture environment regulation after insemination and statistics of insemination success rate. The method has the advantagesthat in the sebastes schlegeli male and female parent fish mating season, a small amount of sperms can be obtained, the female parent fish insemination process can be rapidly completed, the female parent fish insemination success rate is 91% or above, and the problem that the mating rate is low in the mating process due to improper condition control during indoor artificial parent fish breeding issolved; the method overcomes the defect that wild or net cage parent fish is adopted for breeding at present, can achieve full-artificial breeding of sebastes schlegeli and batch production of offspring seeds, and also provides technical support for sebastes schlegeli breeding, culture, proliferation and releasing.

Description

technical field [0001] The invention belongs to the technical field of seawater fish breeding in aquaculture, and in particular relates to an artificial insemination method for the in vivo fertilization fish Scorpius xushii. Background technique [0002] Xu's flat scorpionfish ( Sebastes schlegelii ), also known as black scorpion, belongs to Scorpioformes - Scorpionidae - Scorpene genus. It is mainly distributed in the Yellow Sea, Bohai Sea and East China Sea in my country. It is also common in the Korean Peninsula, Japan and the Sea of ​​Okhotsk. It is a cold-temperate bottom rock reef in the offshore sexual fish. Its strong disease resistance, broad food and natural overwintering and other breeding characteristics, tender and delicious, rich in nutrition and other nutritional value, two advantages make it become an important new cage culture object in the northern coast of my country. Xu's flat scorpionfish adopts the oviparous reproductive habit of internal fertilizati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/02A01K61/10
CPCA61D19/027A01K61/10Y02A40/81
Inventor 韩慧宗王腾腾姜海滨崔广鑫王斐张明亮陈钰臻刘丽娟牛志兵
Owner 山东省海洋资源与环境研究院
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