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Crossbreeding method of hypoxia-resistant epinephelus awoara

A technology of blue grouper and hybrid breeding, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of low breeding efficiency and slow growth of blue grouper, and achieve high market acceptance, considerable economic benefits, and significant The effect of growth advantage

Active Publication Date: 2021-03-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for hybrid breeding of low-oxygen-resistant blue grouper, aiming to solve the problem that the blue grouper in the prior art grows slowly and causes its breeding benefit to be low

Method used

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  • Crossbreeding method of hypoxia-resistant epinephelus awoara

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment one blue body large grouper and blue grouper are cultivated

[0061] The breeding environment of blue-bodied grouper is factory farming. The size of the breeding pool is 6m×6m×3m. There are 30 blue-body grouper in each pond, and a total of 80 blue-body grouper. 50-70cm, weight 20-30Kg. The dissolved oxygen (DO) in the pool is 7-10mg / L, and the water exchange volume is 3-4m 3 / h, using natural light. The water temperature is 23-24°C in October of the first year, and gradually drops to 18-20°C in December, and then gradually rises to 25-26°C in March of the next year; When the water temperature is stable at 25-26°C, mature male fish can be selected to collect semen. Wherein, the bait fed to the parents is any one of mackerel and mackerel or mixed in any proportion of the two, and the feeding amount is about 2-3% of the body weight of the parent fish. Pour the pool regularly every day to keep the water fresh.

[0062] The blue grouper is cultured in cages, ...

Embodiment 2

[0063] Example 2 Cryopreservation of the sperm of the blue-bodied grouper

[0064] (1) Acquisition of mature sperm

[0065] Under the artificially controlled environment, the sperm production time of the blue-bodied grouper extends from April to mid-September. At this time, the precious and small amount of blue-bodied grouper sperm can be preserved for a long time by freezing sperm technology, which can be used Insemination with blue grouper eggs in the following year.

[0066] The semen is collected every half month during the reproductive season, and the male fish that have collected the semen are put into another fish pond with fresh water quality. Before semen collection, MS-222 was used to anesthetize the whole pool. The male fish was placed on the delivery bed, and the abdomen was squeezed by hand. Mature males with semen flow were selected, and the semen was obtained by squeezing the abdomen. During the process of semen extraction, sea water should be avoided, otherwi...

Embodiment 3

[0075] Example 3 Artificial insemination of blue-bodied grouper frozen sperm and blue grouper eggs

[0076] (1) blue grouper induced labor

[0077] When the water temperature reaches 23-26°C in April, select a well-developed female blue grouper with a swollen abdomen; inject chorionic gonadotropin and gonadotropin-releasing hormone analogs into the base of the pectoral fins, and the dose of chorionic gonadotropin is 200 -300IU / kg, the dose of gonadotropin-releasing hormone analogue is 35μg / kg, the two are mixed in 0.9% normal saline, and each fish is injected about 1mL. After 48-60 hours of injection, suck out the eggs through a straw to observe the development status, select mature female fish, place them on the spawning bed, squeeze the abdomen of the fish to collect eggs, and then collect the eggs in a dry plastic basin, and obtain a total of 10Kg of eggs .

[0078] (2) Fertilization of eggs with frozen sperm

[0079] During the 48-60 hours after the blue grouper is inje...

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Abstract

The invention discloses a crossbreeding method of hypoxia-resistant epinephelus awoara. The method comprises the following steps: 1) parent fish cultivation of epinephelus tukula and acquisition of sperms; 2) sperm cryopreservation; 3) parent fish cultivation of epinephelus awoara and ovum acquisition; 4) artificial insemination: frozen seminal fluid of the epinephelus tukula is taken, after beingunfrozen, the seminal fluid is uniformly mixed with ova, and seawater is added for fertilization; standing is performed, high-salinity seawater is added, and floating fertilized eggs are collected; (5) hatching management: hatching is conducted for 6-10 hours, the fertilized eggs are added into the high-salinity seawater, the floating fertilized eggs are collected again and put into a culture pond for hatching and culturing; and (6) later-period cultivation: biological baits are utilized for cultivation to obtain epinephelus awoara hybrid fries. According to the crossbreeding method, the fries obtained through crossbreeding of the epinephelus awoara is high in survival rate, high in growth speed, resistant to low oxygen and low temperature and uniform in filial generation form and body color, the chromosome karyotype is 2n=1sm+47t, and the epinephelus awoara has unique hereditary features and remarkable hybridization advantages, and is high in market acceptance degree and considerablein economic benefit.

Description

technical field [0001] The invention belongs to the technical field of fish cross-breeding, in particular to a method for cross-breeding hypoxia-tolerant blue grouper. Background technique [0002] Grouper is the main marine farmed and captured fish in the world. In 2019, the production of grouper aquaculture and fishing in my country reached 183,127 tons and 97,411 tons respectively, ranking second in marine cultured fish, with an output value of more than 20 billion yuan. [0003] Blue grouper (Epinephelus awoara) belongs to Perciformes (Perciformes), grouper family (Epinephelidae), grouper genus (Epinephelus) in taxonomy, and its common name is earth spot. Blue grouper is a kind of high-grade rare fish that inhabits the middle and lower layers of the warm water. It is often distributed in the western part of the North Pacific Ocean. In my country, it is mostly distributed in the South China Sea and the southern part of the East China Sea. [0004] Green grouper has high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 田永胜陈帅王林娜李振通李子奇黎琳琳刘阳
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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