In-vitro metabolic activity detection system based on intestinal microorganisms

A technology of intestinal microorganisms and metabolic activity, which is applied in the field of intestinal microorganism detection, can solve the problems of systems lacking intestinal microorganisms, etc., and achieve the effect of fast and accurate sequencing

Pending Publication Date: 2021-03-09
贾卫国
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Problems solved by technology

One of the main causes of prediabetes is insulin resistance, which may have different pathogenesis for underweight and overweight prediabetes, and the intestinal flora, especially its metabolites, is an important reason; therefore, there is a lack of a method that can detect intestinal microbial system

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  • In-vitro metabolic activity detection system based on intestinal microorganisms

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Embodiment Construction

[0037] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0038] according to figure 1 As shown, the present invention provides an in vitro metabolic activity detection system based on intestinal microorganisms, which is characterized in that it comprises the following steps:

[0039] Obtain a stool sample and screen the stool sample with a stool analyzer;

[0040] According to the screening results, retain stool samples without organic lesions;

[0041] Viability testing on stool samples without organic disease;

[0042] According to the activity test results, the metabolic value of living intestinal bacteria;

[0043] According to the comparison between the metabolic value of intestinal bacteria and the pre-stored inf...

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Abstract

The invention provides an in-vitro metabolic activity detection system based on intestinal microorganisms. The in-vitro metabolic activity detection system comprises the following steps of acquiring excrement samples, and screening the excrement samples through an excrement detector; according to a screening result, reserving excrement samples without organic lesions; carrying out activity detection on the excrement samples without the organic lesions; detecting the metabolic value of live intestinal bacteria according to an activity detection result; comparing the metabolic value of the intestinal bacteria with pre-stored information, and performing gene sequencing on the intestinal bacteria with large difference in the comparison value; determining structural characteristics of intestinal flora according to a gene sequencing result; and obtaining a biomarker at the early stage of diabetes according to the structural characteristics of intestinal flora. An intestinal microorganism in-vitro metabolic activity detection system is used for carrying out in-vitro fermentation on excrement of different subtypes and normal people, metabolic response values corresponding to different prebiotics are detected, including gas production, acid production, degradation degree of the prebiotics and promotion effect on probiotics, and therefore, the overall metabolic level of intestinal microorganisms is comprehensively reflected.

Description

technical field [0001] The invention relates to the technical field of intestinal microorganism detection, in particular to an in vitro metabolic activity detection system based on intestinal microorganisms. Background technique [0002] At present, the incidence of prediabetes is very high, but the degree of attention and treatment adherence rate is very low. Unfavorable control of pre-diabetes will lead to a significant increase in the incidence and mortality of diabetes and cardiovascular diseases in my country, and will bring a catastrophic blow to my country's fragile health economy. [0003] A large number of studies have shown that gut microbes play an important role in the occurrence and development of diabetes. One of the main causes of prediabetes is insulin resistance, which may have different pathogenesis for underweight and overweight prediabetes, and the intestinal flora, especially its metabolites, is an important reason; therefore, there is a lack of a metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12Q1/689C12Q1/06G16B30/10
CPCC12Q1/6869C12Q1/689G16B30/10C12Q2535/122
Inventor 贾卫国
Owner 贾卫国
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