Clenbuterol, ractopamine, salbutamol three-in-one enzyme-linked immunoassay and special kits and detection methods of kits

Abstract

The invention discloses a clenbuterol, ractopamine and salbutamol disposable method for euzyme linked immunosorbent assay detection, which is characterized in that a monoclonal antibody is precoated on an enzyme label plate, clenbuterol, ractopamine and salbutamol in a sample to be detected and enzyme-labelled antigen compete for binding with the coated monoclonal antibody, a developer is used for developing, the content of clenbuterol, ractopamine and salbutamol in the sample to be detected is in inverse proportion with percent absorbency of the sample to be detected, compared with a standard curve, the content of clenbuterol, ractopamine and salbutamol in the sample to be detected can be obtained, and a special detection kit used for the above mentioned detection method comprises the enzyme label plate, a standard substance solution, a concentrated enzyme marker, an enzyme marker diluents, the developer, a concentrated washing lotion and a stopping solution. The invention uses a direct competition enzyme linked immunosorbent assay technology, three kinds of clenbuterol hydrochloride which are clenobuterol hydrochloride, ractopamine and salbutamol are performed with disposable analysis detection, the usage is simple, the specificity is high, and the detection scope is 0.1ng / mL-2mumg / mL.

Description

technical field [0001] The invention relates to the technical field of beta stimulant detection, in particular to an enzyme-linked immunoassay for one-time detection of clenbuterol, ractopamine and salbutamol, a special detection kit and a detection method thereof. Background technique [0002] With the continuous improvement of people's living standards, people begin to pay more and more attention to their health. In daily life, people often cook meat to enhance their immunity and supplement nutrition. When eating food, people often worry about a question-"Does meat food contain beta stimulants (clenbuterol)?" Beta stimulants are pharmaceutical additives that can promote lean meat growth, such as clenbuterol, Ractopamine, albuterol, etc. It can increase the growth rate of pigs, increase the percentage of lean meat, and can make the color of pig hair ruddy and bright, and it looks good; after slaughter, the color of the meat is bright red, and the fat layer is very thin. T...

AI Technical Summary

Problems solved by technology

Radioimmunoassay technology has the advantages of strong specificity, high sensitivity, accuracy, speed, easy operation, and easy standardization, etc., but the instruments used are expensive, and the radioisotopes used are irradiated and polluted, and the waste is not easy to handle, which limits this method. Wide application of the method; gold standard immunoassay technology can screen a large number of samples, but its false positive rate is high, the detection sensitivity is insufficient, the stability is insufficient, and it cannot provide accurate results

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