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Bifidobacterium bifidum i771, its isolation and purification method and application

A Bifidobacterium bifidum and microbial agent technology, applied in the field of bioengineering, to achieve the effect of regulating the balance of intestinal flora, increasing the aroma of fermented milk, and maintaining intestinal stability

Active Publication Date: 2021-11-19
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is little research on the relationship between Bifidobacterium and cortisol; Chinese invention patent CN110331119A, "Bifidobacterium bifidum CCFM1063 and its application" discloses the effect of Bifidobacterium bifidum CCFM1063 on the abundance of other bacterial genera in the intestinal tract. Influence, and then play a role in reducing the occurrence of diseases such as anxiety, depression and enteritis, but there is no research on the relationship between bifidobacteria and cortisol

Method used

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  • Bifidobacterium bifidum i771, its isolation and purification method and application
  • Bifidobacterium bifidum i771, its isolation and purification method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Embodiment 1 Bifidobacterium bifidum i771 and its separation and purification method

[0052] 1. Species information

[0053] Bifidobacterium bifidum i771 is isolated and screened from the intestinal flora or feces of breast-fed infants or young children. This strain can regulate the balance of intestinal flora and promote health; this strain is preserved in the Chinese Microbial Culture Collection Management The General Microbiology Center of the Committee, the preservation address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, the preservation date is May 20, 2020, the preservation number is CGMCC NO.19855, and the Latin name is Bifidobacterium bifidum.

[0054] 2. Bifidobacterium bifidum i771 and its isolation and purification method

[0055] The separation and purification method is carried out according to the following sequence of steps:

[0056] Step 1. Collect samples

[0057] Feces of breast-fed infants or young children were taken...

Embodiment 2

[0070] The bacteriological characteristic of embodiment 2 Bifidobacterium bifidum i771

[0071] 1. Strain characteristics experiment

[0072] The Bifidobacterium bifidum strain isolated and purified in Example 1 was anaerobically cultured at 37° C. for 48 hours, and the strain characteristics were observed and tested respectively. The basic characteristics of Bifidobacterium bifidum i771 provided by Example 1 are shown in Table 1:

[0073] Table 1. Basic characteristics of Bifidobacterium bifidum i771

[0074] experimental project result experimental project result a positive Cell morphology polymorphic rod Oxidase - Catalase -

[0075] Note: "-" means no relevant basic characteristics.

[0076] 2. Experiments on the characteristics of sugar fermentation

[0077] Pick a single colony from the above-mentioned isolated and purified strains for streaking on the plate, culture anaerobically at 37°C for 36-48 hours, passage once, pick a sin...

Embodiment 3

[0087] Example 3 Intervention experiment of Bifidobacterium bifidum i771 on cell model of depression

[0088] 5 x 10 per well 4 SH-SY5Y cells in good condition were inoculated in a 96-well plate containing 200 μL RPMI1640 complete medium (10% fbs) per well, and cultured until the cells covered the bottom by 60-80%. Set up blank control group, cortisol intervention group and experimental group respectively, with 3 repetitions in each group. The cortisol intervention group and the experimental group were stimulated with 300 μmol / L cortisol and treated for 24 hours at 37°C to complete the preparation of depression model cells. Use 10 respectively 7 、10 6 、10 5 Bifidobacterium bifidum i771 with three measurements of CFU was used to intervene the depression model cells at 37°C for 4 hours. Finally, the cell viability was detected by CCK8 method.

[0089] CCK8 method for cell viability detection method: seeding concentration in 96-well plate is 2×10 4 cells / mL of the three ce...

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Abstract

The invention belongs to the field of bioengineering and discloses a Bifidobacterium bifidum i771, a separation and purification method and application thereof. The bacterial strain is screened from infant feces, and is preserved in the General Microorganism Center of the China Microorganism Culture Collection and Management Committee. The number is CGMCC NO.19855; the Bifidobacterium bifidum i771 is obtained by enriching, screening and continuously purifying the target strain in the feces of breast-fed infants or young children. The strain obtained by the invention can adjust the balance of intestinal flora, treat or relieve depression; at the same time, in the aspect of yogurt fermentation, it can also increase the aroma of fermented milk and improve the preference of yogurt; the strain can be used for preparing fermented dairy products and as a treatment and / or Use of the active ingredient in a drug for the relief of depression.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a bacterial strain, its screening method and application, specifically, a bifidobacterium bifidum i771, its separation and purification method and application. Background technique [0002] Depression is the fourth most common disease in the world. The science popularization, prevention and treatment of depression need to be paid more attention to. The prevention and treatment of depression has been listed as the national mental health work priority. Pathogen infection can activate the immune system, release toxins and cytokines, thereby affecting host behavior, metabolism and neurotransmitter function, and the released cytokines can induce neuroendocrine and central nervous system chemical changes, thereby triggering depression. It is shown in the prior art that lactic acid bacteria may improve depression through multiple pathways and multiple targets. Lactic acid bact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A23C9/123A23L33/135A61K35/745A61P25/24C12R1/01
CPCC12N1/02C12N1/20A23C9/1234A23L33/135A61K35/745A61P25/24A23V2002/00A23V2400/517A23V2200/30Y02A50/30
Inventor 张栋魏立华袁庆彬王世杰冯丽莉薛玉玲荀一萍封肖颖刘尧尧
Owner JUNLEBAO DAIRY GRP CO LTD
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