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Salvia miltiorrhiza root extract as well as preparation method and application thereof

A technology of extract and salvia miltiorrhiza, applied in the field of medicine, to achieve obvious medicinal prospects and clinical application value, significant therapeutic effect, and the effect of suppressing toxicity

Pending Publication Date: 2021-03-05
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The currently reported Danshen extract achieves antibacterial effect by inhibiting the growth of bacteria, but there is no relevant report on the use of Danshen extract in the preparation of anti-virulence drugs against Staphylococcus aureus

Method used

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  • Salvia miltiorrhiza root extract as well as preparation method and application thereof
  • Salvia miltiorrhiza root extract as well as preparation method and application thereof
  • Salvia miltiorrhiza root extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of salvia miltiorrhiza extract

[0039] a) After pulverizing the Danshen medicinal material (Danshen decoction pieces) into a coarse powder, use 10 times the volume fraction of 80% ethanol to reflux extract for 3 times, each extraction for 1.5 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to No alcohol smell, get the crude extract of Salvia miltiorrhiza;

[0040]b) Suspend the obtained crude extract of Salvia miltiorrhiza in water, extract the obtained suspension with an equal volume of petroleum ether for 3 times, then extract with ethyl acetate for 3 times, discard the petroleum ether extract, and combine the ethyl acetate The extract was concentrated under reduced pressure to obtain the Danshen extract (abbreviated as SM3-2).

[0041] Through UPLC analysis: the obtained Danshen extract SM3-2 does not contain tanshinone Ⅱ A and cryptotanshinone (see Figure 1 to Figure 5 shown).

Embodiment 2

[0042] Example 2: Effect experiment of Salvia miltiorrhiza extract on the growth of Staphylococcus aureus

[0043] Pick a single clone of Staphylococcus aureus, add it to tryptone soybean broth medium (TSB), and culture it overnight in a constant temperature shaker at 37°C at a speed of 250rpm; the bacterial solution is diluted 100 times with fresh TSB medium ℃ constant temperature shaker, culture at 250rpm for about 2.5 hours to OD 600 When it reaches 0.2-0.3, subpackage, add SM3-2 (final concentration is 400μg / mL), add the same volume of DMSO to the control group, mix well, take 150μL of bacterial solution to measure OD at 0 hours 600 , and the remaining bacterial liquid was cultured on a constant temperature shaker at 37°C for 12 hours, during which 150 μL of bacterial liquid was taken out every 2 hours to measure OD 600 , draw the growth curve of the strain; the experimental results are as follows Image 6 and Figure 7 shown.

[0044] Image 6 The effect of Danshen e...

Embodiment 3

[0045] Example 3: Inhibitory experiment of Danshen extract on hemolytic activity of Staphylococcus aureus

[0046] Pick a single clone of Staphylococcus aureus Newman strain, add it to TSB medium, and culture it in a constant temperature shaker at 37°C at a speed of 250rpm overnight; the bacterial solution is diluted 100 times with fresh TSB medium and placed in a constant temperature shaker at 37°C to Cultivate at 250rpm for 3 hours, subpackage, add different concentrations of drugs, add the same volume of DMSO to the control group, and continue to cultivate for 3 hours on a constant temperature shaker at 37°C; then centrifuge the bacterial culture (5500×g, 4°C, 1 minute ), collect the bacterial supernatant; take a 1.5mL centrifuge tube, add 875μL phosphate-buffered saline (PBS), 100μL bacterial supernatant, 25μL defibrinated sheep blood, mix well, incubate at 37℃ for 3 hours and then centrifuge (5500× g, room temperature, 1 minute); then take 150 μL of the supernatant, and m...

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Abstract

The invention discloses a salvia miltiorrhiza root extract as well as a preparation method and application thereof. The salvia miltiorrhiza root extract is prepared by the following steps: extractingsalvia miltiorrhiza roots with a mixed solvent of a hydrophilic solvent and water to obtain crude extract extractum, then suspending the crude extract extractum in water, performing leaching with petroleum ether and then performing leaching with ethyl acetate to obtain an ethyl acetate leaching part, namely the salvia miltiorrhiza root extract, wherein the salvia miltiorrhiza root extract does notcontain tanshinone IIA and cryptotanshinone. Research results show that the salvia miltiorrhiza root extract provided by the invention can inhibit the virulence of staphylococcus aureus and reduce the pathogenicity of the staphylococcus aureus under the condition of not inhibiting the growth of the staphylococcus aureus, does not easily generate drug resistance, has a remarkable treatment effecton staphylococcus aureus infection, is expected to be used as an active component for preparing drugs for resisting the virulence of the staphylococcus aureus, and lays a foundation for research and development of novel antibacterial drugs.

Description

technical field [0001] The invention relates to a salvia miltiorrhiza extract and its preparation method and application, belonging to the technical field of medicine. Background technique [0002] Staphylococcus aureus is the most common pathogen that causes suppurative infection in humans. It can directly cause local suppurative infection, pneumonia, pseudomembranous enteritis, pericarditis, etc., and even systemic infection such as sepsis and sepsis, seriously endangering human beings. life and health. The types of Staphylococcus aureus infections can be divided into hospital-acquired infections and community-acquired infections, and the discovery of the latter increases the potential biohazardousness of this pathogen and the possibility of causing infection outbreaks. Antibiotic therapy is the main treatment for Staphylococcus aureus infection, but in recent years due to the increase in the infection rate of Staphylococcus aureus, the acceleration of drug resistance, an...

Claims

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Application Information

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IPC IPC(8): A61K36/537A61P31/04A61K125/00
CPCA61K36/537A61K2236/333A61K2236/53A61P31/04
Inventor 徐宏喜张洪蒋嘉明陈俞宇万世杰谭红胜张莉
Owner SHANGHAI UNIV OF T C M
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