Application of papaya saponin ⅶ in the preparation of msn protein inhibitor

A technology of papaya saponin and its use, which is applied in the application field of papaya saponin VII in the preparation of MSN protein inhibitors, and can solve the problems of unclear function of the target protein

Active Publication Date: 2022-01-11
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, PP7 is a broad-spectrum antitumor drug with important potential for clinical development, but the role of PP7 in multiple myeloma and cancer stem cells and the role of target proteins are still unclear

Method used

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  • Application of papaya saponin ⅶ in the preparation of msn protein inhibitor
  • Application of papaya saponin ⅶ in the preparation of msn protein inhibitor
  • Application of papaya saponin ⅶ in the preparation of msn protein inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Paprika saponin VII was identified as an ideal natural compound monomer for the treatment of multiple myeloma

[0024] Paprika saponins VII (PP7), Paprika papaya VI (PP6), and Paprika papaya I (PP1) of the saponin series (all purchased from Selleck Biotechnology Co., Ltd., USA) have antitumor activity. We first treated MM cells MM.1S (from ATCC), ARP1, ANBL6 and ANBL6-BR cells (all from Professor Zhou Wen, Institute of Cancer Research, Central South University) with these three monomers at a concentration of 2 μM, expecting to obtain Natural product monomers with optimal antitumor activity. After proving that the anti-tumor effect of saponin VII is the most significant, the CCK-8 method was used to detect the effect of saponin VII on immortalized B lymphocytes (from the Genetics Laboratory of School of Life Sciences, Central South University), MM cell lines, and normal human PBMC cells ( From Xiangya Hospital of Central South University) and MM patients with...

Embodiment 2

[0051] Example 2: Paprika saponin VII inhibits multiple myeloma stem cell-like cells and targets side population cells.

[0052] Flow cytometry was used to detect the effect of saponin Ⅶ on the ratio of side population cells of ARP1 and RPMI-8226 cells, and the effect of saponin Ⅶ on ARP11 and RPMI-8226 cell stemness genes Oct4, Nanog, Sox2, Lin28 and Klf4 were detected by qPCR. After the side population and main population of ARP1 cells were sorted by flow cytometry, CCK-8 was used to detect the effect of saponin Ⅶ on the survival rate of the side population and the main population of cells, and the soft agar colony formation assay was used to detect the effect of saponin Ⅶ on the side population of cells and the clonogenicity of main population cells. And the effect of saponin Ⅶ on primary CD138 in patients with MM was detected by flow cytometry + Effect of side population cell ratios.

[0053] The experimental steps are as follows:

[0054] 1. Flow cytometric detection o...

Embodiment 3

[0095] Example 3: Paprika saponin VII inhibits MSN protein

[0096] DARTS (Drug Affinity Response Target Stability Method) was used to search for the target protein of saponin Ⅶ. After the samples were treated with the DARTS method and CETSA (cell thermal transition analysis) method, WB was used to verify whether the MSN protein was the target protein of papaya saponin VII, and then WB was used to detect the difference in the expression of MSN in normal human immortalized B lymphocytes and MM cell lines, WB directly detected the changes of MSN protein after the treatment of ARP1 and MM.1S cells with saponin Ⅶ. Studies have found that small-molecule drugs will stabilize the protein conformation after binding to the protein, making it resistant to protease hydrolysis. The DARTS method was invented based on this principle. The drug ligand will also induce the thermal stability of the target protein, and the CETSA method was invented based on this principle.

[0097] 1. DARTS me...

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PUM

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Abstract

The invention discloses the application of saponin VII in the preparation of MSN protein inhibitor. Through series of experiments, it is proved that the saponin VII of papaya has anti-multiple myeloma activity. And saponin VII of pagoda plant exerted anti-multiple myeloma activity by directly inhibiting the protein level of MSN and targeting the side population cells of multiple myeloma stem cell-like cells. Papaya saponin VII can be used as an inhibitor of MSN protein to treat diseases caused by increased expression of MSN protein, such as multiple myeloma, and is expected to be developed as a new type of antitumor drug.

Description

technical field [0001] The present invention relates to a new application of papaya saponin VII, in particular to the application of papaya saponin VII in the preparation of MSN protein inhibitors. It belongs to the field of medical technology. Background technique [0002] MSN (moesin), a member of the ezrin-radixin-moesin (ERM) protein family, is a protein that links the actin cytoskeleton to transmembrane receptors. The physiological role of ERM proteins is related to the structure of the cell cortex and participates in cell differentiation and morphogenesis. MSN is upregulated in a variety of human cancers, including breast cancer, prostate cancer, pancreatic cancer, and lung and melanoma. MSN is the dominant ERM protein in lymphocytes and is associated with T and B cell efflux in secondary lymphoid organs. CD44 can provide a link between the plasma membrane and the actin cytoskeleton by binding to ERM proteins (Ezrin, radixin, moesin). The MSN-CD44 interaction is a p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P19/08A61P35/00
CPCA61K31/7048A61P19/08A61P35/00
Inventor 刘静王海勤萧小鹃朱林李珍珍易慧
Owner CENT SOUTH UNIV
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