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Application of synanthrin and beneficial bacteria in preventing and controlling tomato soil-borne bacterial wilt

A technology for beneficial bacteria and bacterial wilt disease, applied in the field of microorganisms, can solve problems such as limited nutrients, and achieve the effects of improving inhibitory ability, promoting growth and antagonistic competition ability, and reducing morbidity.

Active Publication Date: 2021-02-26
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the nutrients in the soil are limited, and when the beneficial bacteria are used to prevent and control tomato soil-borne diseases, it is often difficult for the beneficial bacteria to proliferate in large quantities in the soil and exert their due control effects.

Method used

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  • Application of synanthrin and beneficial bacteria in preventing and controlling tomato soil-borne bacterial wilt
  • Application of synanthrin and beneficial bacteria in preventing and controlling tomato soil-borne bacterial wilt
  • Application of synanthrin and beneficial bacteria in preventing and controlling tomato soil-borne bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The Indoor Effect of Inulin on Promoting the Growth of Beneficial Bacteria

[0024] NA liquid medium: glucose 10g, peptone 5g, beef extract 3g, yeast extract 0.5g, deionized water 1000mL, adjust pH to 7.2-7.4, autoclave at 115°C for 30min.

[0025] NA solid medium is NA liquid medium plus 2-3% (w / w) agar.

[0026] The bacterial strain NJHR92 is classified as Lysinibacillus sphaericus, and the strain preservation number is CGMCC No.6629.

[0027] NJHR92 preserved in glycerol was streaked on NA solid medium and cultured at 30°C for 2 days; a single colony on the plate was picked and transferred to NA liquid medium, and cultured overnight at 30°C and 170rpm. Get fresh bacterium liquid, 4500rpm room temperature centrifugation 5min, collect thalline, wash thalline 3 times with sterile normal saline (0.85% NaCl), remove culture medium, adjust with sterile normal saline to obtain concentration to be 1 * 10 8 CFU / mL NJHR92 bacterial suspension, ready for use.

[0028] 2 trea...

Embodiment 2

[0034] Effect of Inulin on Enhancing the Antagonistic Competitive Ability of Beneficial Bacteria

[0035] Material: Ralstonia solanacearum QL-Rs1115 isolated from Qilin Town, Nanjing [3] , its red fluorescent protein marker strain QL-RFP is the model invading pathogenic bacterium of the present invention, and this bacterial strain is that red fluorescent protein gene mCherry is transferred into R. solanacearum QL-Rs1115 through plasmid pYC12, can be in the NA of 30 μ g / mL gentamicin Media plate [4] Grow on it and name it QL-RFP. Beneficial bacteria NJHR92 screened from Qilin Town, Nanjing, can inhibit the growth of Ralstonia solanacearum QL-RFP.

[0036] Ralstonia solanacearum QL-RFP was recovered on NA solid medium containing 30 μg / mL gentamicin. Place the plate in a 30°C incubator and incubate for 2 days until a single colony appears. Pick a single colony on the plate and transfer it to NA liquid medium, and culture overnight at 30°C and 170rpm. Get fresh bacterium liqu...

Embodiment 3

[0044] Inulin enhances the indoor effect of beneficial bacteria against soil-borne Ralstonia solanacearum

[0045] Material: Ralstonia solanacearum QL-RFP tagged with red fluorescent protein. Beneficial bacteria NJHR92 screened from Qilin Town, Nanjing, can inhibit the growth of Ralstonia solanacearum QL-RFP.

[0046] Streak the beneficial bacteria NJHR92 preserved in glycerol on the NA solid medium and culture at 30°C for 2 days; pick a single colony on the plate and transfer it to the NA liquid medium, and cultivate overnight at 30°C and 170rpm. Get fresh bacterium liquid, 4500rpm room temperature centrifugation 5min, collect thalline, wash thalline 3 times with sterile normal saline (0.85% NaCl), remove culture medium, adjust with sterile normal saline to obtain concentration to be 1 * 10 8 CFU / mL NJHR92 bacterial suspension, ready for use.

[0047] Ralstonia solanacearum QL-RFP was recovered on NA solid medium containing 30 μg / mL gentamicin. Place the plate in a 30°C in...

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Abstract

The invention discloses application of synanthrin and beneficial bacteria in preventing and controlling tomato soil-borne bacterial wilt. The synanthrin and the beneficial bacteria are applied to soilby adopting a root irrigation method; wherein the beneficial bacteria are strains NJHR92, are classified and named as Lysinibacillus sphaericus, and are preserved in the China General MicrobiologicalCulture Collection Center on September 26, 2012, the preservation unit address is Beijing Datun Road, Chaoyang District, China General Microbiological Culture Collection Center, and the strain preservation number is CGMCC No. 6629. The beneficial bacteria and the synanthrin are simultaneously applied in a resource regulation and control mode, and the synanthrin is used as a resource substance, sothat the growth and antagonistic competitive capacity of the beneficial bacteria can be promoted, and the inhibition capacity of the beneficial bacteria on ralstonia solanacearum can be remarkably improved, thereby reducing the incidence rate of tomato bacterial wilt and preventing and controlling tomato soil-borne diseases.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to the application of resource-coordinated beneficial bacteria to prevent and control tomato bacterial wilt, and in particular to the application of inulin and beneficial bacteria NJHR92 to control and control tomato soil-borne bacterial wilt. Background technique [0002] Tomato bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum (referred to as Ralstonia solanacearum). production cuts. In recent years, the biological control method of using beneficial bacteria in the rhizosphere to control tomato soil-borne diseases has attracted attention and has been gradually applied in the agricultural production process. However, the applied beneficial bacteria often cannot exert a stable control effect in the soil. One of the reasons is that the beneficial bacteria lack suitable nutrients in the rhizosphere soil environment and cannot effectively grow and colonize, which ...

Claims

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Application Information

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IPC IPC(8): A01N63/20A01N43/16A01P1/00C12N1/20C12R1/07
CPCA01N63/20A01N43/16C12N1/20C12R2001/07C12N1/205
Inventor 韦中杨天杰王震韩岗徐阳春沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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