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A kind of lactobacillus paracasei and application thereof

A technology of Lactobacillus and paracheese, which is applied to the field of microbial fermentation in livestock feed, can solve the problem of not taking into account the low temperature conditions during the harvest period of rice straw, and achieves improved aerobic stability and feed safety, improved fermentation quality, and improved palatability effect

Active Publication Date: 2021-09-17
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent document "A Plantar Lactobacillus Suitable for Silage Straw and Its Application Method (CN201210115193.6)" provides a plantarum lactobacillus that can accelerate the maturation of straw silage and improve the quality of straw silage, but the silage fermentation is in the storage room. Completed, did not take into account the low temperature conditions during the annual rice straw harvest period, and the pH of the silage did not drop below 4.50 for high-quality silage

Method used

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  • A kind of lactobacillus paracasei and application thereof
  • A kind of lactobacillus paracasei and application thereof
  • A kind of lactobacillus paracasei and application thereof

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Experimental program
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Effect test

Embodiment 1

[0022] The isolation and identification of embodiment 1 Lactobacillus paracasei GRR (Lactobacillus paracasei) GRR

[0023] 1. Separation and purification

[0024] Separation and purification from rice straw to obtain Lactobacillus paracasei GRR, specifically as follows: Weigh 10 g of conventional rice straw samples into 90 ml of sterilized distilled water, shake for 1 h on a constant temperature shaker, and then 10-fold gradient dilution, respectively take 10 -3 、10 -4 and 10 -5 Spread sample dilution on MRS solid medium, incubate for 48 hours, then take it out, pick a single colony growing on MRS according to the size, shape and color of the colony, and carry out catalase test and Gram staining . Catalase-negative and Gram-positive were tentatively identified as lactic acid bacteria, and streaked and purified twice on MRS solid medium. Store at -80°C in MRS liquid medium containing 25% v / v glycerol, and the strain is named Lactobacillus paracasei GRR (Lactobacillus paraca...

Embodiment 2

[0030] The detection of embodiment 2 Lactobacillus paracasei (Lactobacillus paracasei) GRR antibacterial activity

[0031] Strain activation: Inoculate cryopreserved (Lactobacillus paracasei) GRR (hereinafter referred to as GRR) in MRS liquid medium, culture at 37°C for 24 hours, and subculture twice in this way to obtain the activated GRR. 3% of the inoculum was re-transferred into the MRS liquid medium, cultured for 24 hours, and the bacterial solution was centrifuged to obtain the supernatant.

[0032]Bacteria (Escherichia coli, Staphylococcus aureus, Salmonella) and fungi (Aspergillus flavus) were used as indicator bacteria, nutrient agar medium was used as a medium for indicator bacteria, and potato dextrose agar medium was used as a medium for indicator fungi. Activate the indicator strain preserved in glycerol. The activated strains were re-transferred to nutrient broth and potato dextrose broth at an inoculum amount of 3%, cultured at 37°C for 24 hours for the indicat...

Embodiment 3

[0035] Embodiment 3: the application of Lactobacillus paracasei (Lactobacillus paracasei) GRR in fermented rice straw

[0036] Preparation of strains: Inoculate cryopreserved GRRs into MRS liquid medium, culture at 37°C for 24 hours, and subculture twice in this way to obtain the activated GRR strains. The activated strains were inoculated in MRS liquid medium and cultivated at 37°C. The bacterial liquid was centrifuged, freeze-dried, pulverized, and mixed with auxiliary materials to make GRR freeze-dried powder, so that the number of viable bacteria in the freeze-dried powder of lactic acid bacteria reached 1×10 10 cfu / g.

[0037] Fermentation of rice straw feed: the rice straw is cut to 3-4cm, the moisture content of the rice straw raw material is controlled at 65%-70%, and 200g of rice straw raw material is weighed and packed into a vacuum-packed plastic bag, and the bacteria powder prepared above is pressed by 0.025 ‰ (i.e. 2.5×10 5 cfu / g) was inoculated into the raw mat...

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Abstract

The invention provides a kind of Lactobacillus paracasei GRR (Lactobacillus paracasei ) GRR, its deposit number is CCTCC No: M2017777. Application of the Lactobacillus paracasei GRR in preparing rice straw fermented feed. The Lactobacillus paracasei of the present invention has the advantages of strong low temperature adaptability, accelerated rice straw fermentation maturation, improved rice straw fermentation quality, improved aerobic stability and feed safety, and can be widely used in the field of rice straw fermented feed preparation.

Description

technical field [0001] The invention discloses a Lactobacillus paracasei and application thereof, and belongs to the technical field of microbial fermentation in livestock feed. Background technique [0002] Ruminants have the characteristics of herbivory, and the guarantee of grass-based roughage has always been a bottleneck restricting the development of the cattle and sheep industry. As far as the regional environment and resource characteristics of agricultural areas are concerned, the use of straw resources as feed is an important way to solve the source of forage grass. [0003] Fermented feed is currently a research and development hotspot in the world. It has shown great prospects in many aspects such as ensuring the safety of feed resources and animal products in our country, promoting emission reduction, and reducing environmental pollution. In southern China, a large number of rice straws are discarded or burned on the spot. With the continuous improvement of ri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/12A23K50/10C12R1/225
CPCA23K10/12A23K50/10C12R2001/225C12N1/205A23V2400/165
Inventor 宦海琳闫俊书刘蓓一丁成龙田吉鹏周维仁
Owner JIANGSU ACAD OF AGRI SCI
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