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A method for establishing the regeneration system of long-tube Lycoris

The technology of a long tube of amaryllis and a system is applied in the field of seedling propagation of herb ornamental plants, can solve the problems of high pollution rate, low proliferation rate, and the rapid propagation method of pollution rate culture has not been established, and achieves the advantages of improving the induction rate and promoting the induction rate. Effect

Active Publication Date: 2022-04-01
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

Previous studies have obtained the proliferation medium and rooting medium of other species of Amaryllis, most of which use bulbs as explants, and often have the problems of high pollution rate and relatively low proliferation rate
However, the rapid propagation method of Amaryllis plant tissue culture with high proliferation rate and low pollution rate has not yet been established.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Explant selection and disinfection: at the end of August, select the apex of the flower axis at the early flowering stage of the long tube of Lycoris as the explant. The disinfection method is to shake with detergent solution for 15 minutes, rinse with running water for 30 minutes, and use it on a sterile operating table. 75% ethanol for 30s, then 0.2% mercuric chloride for 15min, and finally washed with sterile water for 5 times, each wash for 1min, a total of 100 explants were processed;

[0047] 2. Induction of adventitious buds: After detoxification, the explants were cut up and down with the apex of the flower axis as the center and kept 1cm long (the lengths of several branches are equal), and the explants were inoculated in the induction medium MS+6-BA 2mg Adventitious bud induction was carried out in / L +NAA0.2 mg / L, and the culture temperature was controlled at 26°C.

[0048] First cultivate in the dark for 24 hours, then carry out red light culture for 1 da...

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Abstract

The invention provides a method for establishing a regeneration system of Lycoris long tube, which comprises the following steps: firstly, taking the apex of the flower axis of Lycoris long tube at the initial flowering stage as an explant to induce adventitious buds, controlling the culture temperature at 24-26°C, in the dark Medium culture for 24 hours, then red light culture for 1 day + normal culture for 44 days, then cut the obtained long-tube Lycoris adventitious shoots and put them into the subculture medium for 25 days, and finally put the long-tube Lycoris test-tube seedlings into rooting culture Rooting culture was carried out in the base for 15 days. The method can effectively solve the problem of rapid propagation of Lycoris longifolia in vitro.

Description

technical field [0001] The invention relates to a method for culturing and propagating a long tube of Lycoris longiflorum, in particular to a method for establishing a long tube of Lycoris officinalis regeneration system, and belongs to the technical field of the propagation of herb ornamental plant seedlings. Background technique [0002] long tube of Lycoris ( Lycoris longituba ) is a species of the genus Amaryllidaceae. Perennial herbs, with thick underground stems, are excellent perennial herbal flowers. They are often used as shady greening in gardens. They can be used as flower beds or flower diameter materials. They are also beautiful cut flowers. The bulb is poisonous, and it is used as medicine to induce vomiting, expectorant, reduce swelling, and relieve pain. [0003] At present, the number of seedlings of this variety is very small in China. Relevant studies have shown that the reproduction coefficient of lycoris in natural conditions is very low, and seed repr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 束晓春王忠张凤姣庄维兵王涛王宁
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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