Special microbial fertilizer for tobaccos as well as preparation method and application thereof

A technology of microbial fertilizer and microbial inoculum, applied in the application, nitrogen fertilizer, potassium fertilizer and other directions, can solve the problem of inability to balance fertilizer efficiency and resistance to diseases and insect pests, achieve good ecological and economic benefits, promote growth, and promote the effect of fertilizer efficiency.

Inactive Publication Date: 2021-02-09
SHANGLUO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, it solves the problem that the microbial fertilizer can not b

Method used

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  • Special microbial fertilizer for tobaccos as well as preparation method and application thereof
  • Special microbial fertilizer for tobaccos as well as preparation method and application thereof
  • Special microbial fertilizer for tobaccos as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: A kind of special microbial fertilizer for tobacco and preparation method thereof

[0035] A special microbial fertilizer for tobacco, the components of which are calculated in parts by weight, including 10-18 parts of Sophora japonica stalks, 4-12 parts of biomass charcoal, 31-39 parts of superphosphate, 21-29 parts of potassium sulfate, and ammonium nitrate 10-14 parts, 4-8 parts of microbial agent.

[0036] The microbial agent includes Saccharomyces cerevisiae, Streptomyces lavendulae, Bacillus amyloliquefaciens and Trichoderma viride.

[0037] The above-mentioned biochar is prepared by mixing and washing rice husk charcoal and rice straw charcoal according to a mass ratio of 1:1.

[0038] The present invention also discloses a method for preparing the above-mentioned special microbial fertilizer for tobacco, comprising the following steps:

[0039] (1) The slant strains stored in the environment of 0-4°C were activated, Saccharomyces cerevisiae, Stre...

Embodiment 2

[0044] Embodiment two: the preparation of special microbial bacterial fertilizer for tobacco

[0045] The slant strains stored in the environment of 0-4°C were activated, and the four strains of Saccharomyces cerevisiae, Streptomyces lavender, Bacillus amyloliquefaciens and Trichoderma viride were respectively cultured in test tubes and shake flasks, among which Saccharomyces cerevisiae was Optimized FC2 medium, the formula of the medium is: 2% sucrose, 0.4% yeast extract, 0.3% peptone, 0.2% disodium hydrogen phosphate, inoculum size is 1-2%, 150-200rpm, cultured at 37°C for 24-36h Streptomyces lavender adopts the optimized soybean flour medium, the formula of the medium is: starch 1.2%, glycerin 2%, soybean flour 2.1%, glucose 0.6%, yeast extract 0.2%, peptone 0.3%, calcium carbonate 0.2%, corn 2% oil, 10% inoculum, 220rpm, cultured at 35°C for 140h; Bacillus amyloliquefaciens adopts optimized Landy medium, and the formula of the medium is: glucose 2%, L-sodium glutamate 0.5%...

Embodiment 3

[0046] Embodiment three: the preparation of special microbial bacterial fertilizer for tobacco

[0047] Based on Example 2, each bacteria is activated and cultured to obtain a fermentation broth; after the fermentation, Saccharomyces cerevisiae, Streptomyces lavender, Bacillus amyloliquefaciens, and Trichoderma viride are mixed in a volume ratio of 1:1:2:2 to prepare To obtain a composite fermentation bacterial agent; 5 kg of the composite bacterial agent liquid is mixed with 12 kg of Sophora japonica stalks crushed to 1-2 cm and stirred evenly; rice husk charcoal and rice straw charcoal are added to the raw materials according to a mass ratio of 1:1, mixed and cleaned to obtain raw Material charcoal 6kg, superphosphate 33kg, potassium sulfate 23kg, ammonium nitrate 11kg, stir evenly, dry until the moisture content is below 15%, and the number of viable bacteria is not less than 0.5×10 9 cfu / mL, that is, the special microbial fertilizer for tobacco was prepared. When applying...

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Abstract

The invention discloses a tobacco microbial fertilizer. The tobacco microbial fertilizer comprises four strains, namely Saccharomyces cerevisiae, Streptomyces lavendulae, Bacillus amyloliquefaciens and Trichoderma viride. of the tobacco microbial fertilizer. The tobacco microbial fertilizer is prepared from the following steps: preparing the following four strains; The invention further disclosesa method for preparing a decomposed solid fermentation agent. According to the microbial agent provided by the invention, the synergistic effect among the four bacteria is utilized in the tobacco microbial fertilizer to quickly form dominant flora, and the dominant flora is matched with sophora alopecuroide straw and other raw materials to fully decompose the raw materials and promote the exertionof the fertilizer efficiency. Meanwhile, the problem that a microbial fertilizer cannot give consideration to fertilizer efficiency and disease and pest resistance is solved. The prepared microbial fertilizer can significantly promote the growth of tobacco, has significant control effects on tobacco bacterial wilt, tobacco black shank, tobacco mosaic disease, root-knot nematode disease and myzuspersicae, has good ecological benefits and economic benefits, and has huge development potential.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and further relates to a tobacco fertilizer, and further relates to the technical field of a special microbial fertilizer for tobacco and its application. Background technique [0002] Biological fertilizers usually use organic or inorganic fertilizers as carriers to regulate plant growth by adding certain types of special microorganisms and using the joint action of microorganisms and common fertilizers. Biological fertilizer mainly has two functions, one is as a functional fertilizer; the other is to regulate plant growth. Studies have shown that after spraying tobacco foliage or root application of biological bacterial fertilizer, the root system of the plant will develop vigorously, the vitality of the root system will be improved, and the nutrient absorption capacity will be enhanced. Bacterial fertilizer can increase the chlorophyll content of tobacco plants, increase the p...

Claims

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Application Information

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IPC IPC(8): C05G3/60
CPCC05B1/02C05G3/60C05C1/00C05D1/02C05D9/00C05F11/00C05F11/08
Inventor 陈月星陈明山黄金辉李慧心陈曦文佳奇
Owner SHANGLUO UNIV
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