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Method for detecting residual veterinary drug content in dairy products

A detection method and technology of dairy products, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome pre-treatment process, and achieve the effects of less miscellaneous peaks, less pollution, and high purity

Pending Publication Date: 2021-01-22
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for detecting the content of residual veterinary drugs in dairy products, so as to solve the problem of cumbersome pretreatment process required for detecting residual veterinary drugs in dairy products

Method used

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  • Method for detecting residual veterinary drug content in dairy products
  • Method for detecting residual veterinary drug content in dairy products
  • Method for detecting residual veterinary drug content in dairy products

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0062] Example 1 A detection method for residual veterinary drug content in dairy products

[0063] 1) Detection of mixed veterinary drug standards

[0064] Take 1mg of the veterinary drug mixed standard in a 10mL volumetric flask, add methanol to make up to the mark, and then get the veterinary drug standard stock solution (concentration is 100μg / mL); pipette 10μL of the veterinary drug standard stock solution in a 10mL volumetric flask , add methanol to set the volume to the mark, and then obtain the mixed veterinary drug standard intermediate solution (concentration is 100ng / mL).

[0065] Pipette 0 μL, 2.5 μL, 5 μL, 10 μL, 25 μL, 50 μL, and 100 μL of the mixed veterinary drug standard intermediate solution, and prepare a concentration series of 0.0ng / mL, 0.5ng / mL, 1.0ng / mL, and 2.0ng with blank matrix solution / mL, 5.0ng / mL, 10.0ng / mL, 20.0ng / mL matrix mixed standard series working solution, prepared immediately before use.

[0066] The standard liquids of six veterinary ...

Embodiment 2

[0117] The measurement result of embodiment 2:

[0118] (1) Qualitative measurement results:

[0119] In embodiment 2, the retention time of each veterinary drug in the liquid to be tested is as follows: the retention time of triclabendazole is 3.21min, the retention time of niclofenac is 1.80min, the retention time of iodosanamide is 5.76min, and the retention time of chlorocyanidin The retention time of salamide was 5.31min, that of sodium salicylate was 1.06min, and that of toltrazuril was 2.69min. Therefore, it was proved that the residual veterinary drugs contained in the dairy products tested in this test included triclabendazole, niclofenac, iodoxanamide, closantamide, sodium salicylate and tortrazuril.

[0120] (2) Quantitative determination results:

[0121] Substitute the peak area of ​​each component of the veterinary drug measured in the test solution into the linear regression equation in Example 2, and calculate the content of each component of the veterinary d...

Embodiment 3

[0124] The assay result of embodiment 3:

[0125] (1) Qualitative measurement results:

[0126] In embodiment 2, the retention time of each veterinary drug in the liquid to be tested is as follows: the retention time of triclabendazole is 3.21min, the retention time of niclofenac is 1.80min, the retention time of iodosanamide is 5.76min, and the retention time of chlorocyanidin The retention time of salamide was 5.31min, that of sodium salicylate was 1.06min, and that of toltrazuril was 2.69min. Therefore, it was proved that the residual veterinary drugs contained in the dairy products tested in this test included triclabendazole, niclofenac, iodoxanamide, closantamide, sodium salicylate and tortrazuril.

[0127] (2) Quantitative determination results:

[0128] The peak area of ​​each component of the veterinary drug measured in the test solution in Example 3 is substituted into the linear regression equation, and the content of each component of the veterinary drug in the t...

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Abstract

The invention belongs to the field of food detection, and discloses a method for detecting the content of residual veterinary drugs in a dairy product. The method comprises the following steps: extracting the residual veterinary drugs in a dairy product by using acetonitrile, adsorbing by using an activated solid-phase extraction column, drying the obtained effluent by distillation, dissolving byusing acetonitrile, filtering, and determining. According to the method, the activated solid-phase extraction column is used for adsorbing and removing strong hydrophobic interferents and impurities in the dairy product to obtain the target residual veterinary drug, the method is easy and convenient to operate and efficient, the recovery rate of the residual veterinary drug is high, the problem that the pretreatment process required for detecting the residual veterinary drug in the dairy product is tedious is solved, and the method is suitable for detecting the content of residual veterinary drugs in dairy products.

Description

technical field [0001] The invention belongs to the field of food detection and relates to detection of dairy products, in particular to a detection method for residual veterinary drug content in dairy products. Background technique [0002] With the improvement of residents' living standards, people pay more attention to food safety, especially when consuming dairy products. The residual veterinary drugs in milk mainly come from the drugs used in the prevention and treatment of cow diseases. In order to ensure the quality and safety of dairy products, the state has continuously increased the types of veterinary drug residue detection in milk, such as the release and implementation of the GB 31650 and 250 announcements, and the corresponding detection technologies and requirements have also been continuously improved. [0003] At present, the liquid chromatography detection method is used to analyze the residual veterinary drugs, but the liquid chromatography detection meth...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062
Inventor 张敏王瑶柴艳兵张耀广李飞李兴佳苏运聪魏敏马晓冲
Owner JUNLEBAO DAIRY GRP CO LTD
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