Novel aortic aneurysm marker

A technology of aortic aneurysm and markers, applied in the direction of anti-animal/human immunoglobulin, biological testing, biomaterial analysis, etc., can solve insufficient problems, achieve the effect of avoiding deterioration and high sensitivity

Pending Publication Date: 2021-01-08
EIKEN KAGAKU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they are insufficient in terms of sensitivity, specificity and disease specificity

Method used

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  • Novel aortic aneurysm marker
  • Novel aortic aneurysm marker
  • Novel aortic aneurysm marker

Examples

Experimental program
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Effect test

Embodiment 1

[0120]Determination of NPC2 and IGFBP7 from serum samples of patients with thoracic aortic aneurysm (ELISA method)

[0121]The serum concentrations of NPC2 and IGFBP7 were measured by ELISA. NPC2 and IGFBP7 were determined using NPC2 ELISA kit (Aviva Systems Biology) and ELISA kit for insulin-like growth factor binding protein-7 (Cloud-Clone), respectively. Perform the determination in accordance with the operating protocol attached to the kit.

[0122]In detail, in a solid phase plate of anti-NPC2 antibody or anti-IGFBP7 antibody, a diluted serum sample is added and incubated. Then, the sample solution is removed, and biotin-labeled anti-NPC2 antibody is added during NPC2 measurement, and detection reagent A solution is added during IGFBP7 measurement, and incubated. Washing operation is performed after incubation, HRP (Horseradishperoxidase) labeled avidin is added for NPC2 measurement, and detection reagent B solution is added for IGFBP7 measurement, and the incubation is performed. Th...

Embodiment 2

[0130]Determination of TSP1 from plasma samples of patients with thoracic aortic aneurysm (ELISA method) and evaluation of thoracic aortic aneurysm by ROC curve (combination of NPC2, IGFBP7 and TSP1)

[0131]The plasma concentration of TSP1 was measured by ELISA. TSP1 was measured using human thrombin sensitive protein-1 Quantikine ELISA kit (R&D Systems). Perform the determination in accordance with the operating protocol attached to the kit.

[0132]In detail, in the solid phase plate of the anti-TSP1 antibody, the diluted plasma sample was added and incubated. Then, the sample solution is removed, and peroxidase-conjugated anti-TSP1 antibody is added and incubated. After incubation, a washing operation is performed to perform a color reaction, and then a stop solution is added, and the measurement is performed at a main wavelength of 450 nm and a sub-wavelength of 540 nm using a microplate reader (SpectraMax M2e, Molecular Devices). For this data, the concentration was calculated from ...

Embodiment 3

[0139]Determination of PROF1 (ELISA method) from serum samples of patients with thoracic aortic aneurysm and evaluation of thoracic aortic aneurysm markers by ROC curve (combination of NPC2, IGFBP7 and PROF1)

[0140]The serum concentration of PROF1 was measured by ELISA. PROF1 was measured using human prefibrin-1 ELISA kit (CUSABIO technology company). Perform the determination in accordance with the operating protocol attached to the kit.

[0141]In detail, in the solid phase plate of the anti-PROF1 antibody, the diluted serum sample is added and incubated. Then, the sample solution is removed, and biotin-labeled anti-PROF1 antibody is added and incubated. After the incubation, a washing operation is performed, and HRP (horseradish peroxidase)-labeled avidin is added and incubated. Then, perform washing operation, add TMB (3,3',5,5'-tetramethylbenzidine) solution for color reaction, then add stop solution, use microplate reader (SpectraMax M2e, Molecular Devices) in the main The measure...

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Abstract

The purpose of the present invention is to provide a novel biomarker with which it is possible to accurately detect an aortic aneurysm. Specifically, the present invention pertains to an aortic aneurysm detection marker which contains an NPC2 (Niemann-Pick disease type C2) protein and / or an IGFBP7 (Insulin-like growth factor-binding protein 7) protein.

Description

Technical field[0001]The invention relates to the technical field of a clinical inspection agent for assisting the diagnosis of aortic aneurysm. In detail, it relates to aortic aneurysm biomarkers, aortic aneurysm detection agents using biomarkers, aortic aneurysm detection kits, and aortic aneurysm detection methods.Background technique[0002]An aortic aneurysm is a state where a part of the aortic wall is enlarged or protruded around or locally (diameter). Depending on where the aneurys occurs, it is called thoracic aortic aneurysm in the aorta of the chest, and the aorta that is continuous with the abdomen is called the thoracic abdomen An aortic aneurysm is called abdominal aortic aneurysm in the abdomen (Non-Patent Document 1). The peak age of onset of aortic aneurysm is 70-80 years old. With the aging of the population, it has been increasing in recent years, but only by examination, the aortic aneurysm will be discovered and progress basically asymptomatic. Once ruptured, it w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K16/18C07K16/24G01N33/68
CPCG01N33/5308G01N2800/329G01N2333/65G01N33/6893G01N2470/06G01N2333/4745G01N2800/285
Inventor 南野直人八木宽阳锦织充広植田初江松田均村上裕辅
Owner EIKEN KAGAKU
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