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Nucleic acid preserving fluid and preparation method and application thereof

A technology for preserving liquid and nucleic acid, which is applied to biochemical equipment and methods, and the determination/inspection of microorganisms. The effect of good effect and simple preparation method

Active Publication Date: 2021-01-05
广东南芯医疗科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a carrier of biological genetic information, the performance of nucleic acid is unstable, and it is easily affected by external physical factors such as temperature, humidity, ultraviolet rays, etc.; chemical factors such as pH value, hydrolysis reaction, oxidation reaction, etc.; biological factors such as enzymatic hydrolysis and microbial infection. Causes the degradation and denaturation of nucleic acids, which requires strict restrictions on the transportation time of samples, which greatly increases the difficulty of sample storage during transportation
[0004] At present, the way to store samples is mainly to store samples directly at low temperature after collection. This method has great limitations. First, the cost of low temperature storage is high, and special equipment such as liquid nitrogen tanks are required.
The second is to store the sample at ultra-low temperature for a long time, which will easily change the state and structure of the nucleic acid, resulting in inaccurate test results

Method used

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  • Nucleic acid preserving fluid and preparation method and application thereof
  • Nucleic acid preserving fluid and preparation method and application thereof
  • Nucleic acid preserving fluid and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Get the following components of formula quantity and join in the container after sterilization: 21% citric acid, 1.50% Tween 20, 35.90% disodium EDTA, 40% sodium sulfate, 1.50% polyethylene glycol Octyl phenyl ether; add DEPC water to make it dissolve; adjust the pH of the dissolved solution with a pH meter (temperature range is 18-25°C), adjust the pH to 5.0-5.5; pass the above solution through a 0.22 μM water filter membrane to remove Get the bacteria.

Embodiment 2

[0031] Get the following components of formula quantity and join in the container after sterilization: 13% citric acid, 0.50% Tween 20, 13% disodium EDTA, 35% sodium sulfate, 0.50% polyethylene glycol Octyl phenyl ether; add DEPC water to dissolve it; adjust the pH of the dissolved solution with a pH meter (temperature range is 18-25°C) to a pH of 4.5-4.8; pass the above solution through a 0.22 μM water filter membrane to remove Get the bacteria.

Embodiment 3

[0033] Get the following components of formula quantity and join in the container after sterilization: 30% citric acid, 2.50% Tween 20, 15% disodium EDTA, 45% sodium sulfate, 2.50% polyethylene glycol Octyl phenyl ether; add DEPC water to dissolve it; adjust the pH of the dissolved solution with a pH meter (temperature range is 18-25°C) to a pH of 5.7-6.0; pass the above solution through a 0.22 μM water filter membrane to remove Get the bacteria.

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Abstract

The invention discloses a nucleic acid preserving fluid and a preparation method and an application thereof, and belongs to the technical field of biological products. The nucleic acid preserving fluid disclosed by the invention has lower cost of raw materials, fewer components, simple and quick preparation, accurately controlled dosages of additives and reduction of production cost. The nucleic acid preserving fluid can stably preserve nucleic acid samples for 15 days under the transportation condition of 0-50 DEG C without affecting subsequent DNA / RNA extraction and detection experiments ofthe samples, and has a good preservation effect. The nucleic acid preserving fluid can be stably preserved for 2 years under the transportation condition of 0-50 DEG C without affecting subsequent DNA / RNA extraction and detection experiments of the samples, and has long preservation time.

Description

technical field [0001] The present invention relates to the technical field of biological products, and more specifically relates to a nucleic acid preservation solution and its preparation method and application. Background technique [0002] Nucleic acid is a biological macromolecular compound synthesized by many nucleotides, which widely exists in all animal and plant cells and microorganisms. Nucleic acid in organisms can be divided into ribonucleic acid (DNA) and deoxyribonucleic acid (RNA) according to their chemical composition. [0003] Accurate detection of nucleic acid is an important aspect of molecular biology sample analysis. The high cost and high professionalism of nucleic acid detection make sample acquisition and detection usually separate. process. However, as a carrier of biological genetic information, the performance of nucleic acid is unstable, and it is easily affected by external physical factors such as temperature, humidity, ultraviolet rays, etc.;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 张召刁嘉茵马燕郑康帝陈涛
Owner 广东南芯医疗科技有限公司
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