Method for in-situ analysis of squalene content in microbial colonies
A microbial colony and in-situ analysis technology, applied in the field of analysis and detection, can solve problems such as inability to achieve high-throughput in-situ analysis, and achieve the effect of reducing acquisition time and saving time
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Embodiment 1
[0032] A laser electrospray mass spectrometry device for in-situ analysis of a single colony of microorganisms, including a laser 1, an electrospray device 2, a displacement platform 3 and a mass spectrometer 4, the laser 1 is located directly above the displacement platform 3, and the electrospray device 2 is located at the displacement platform Above the front of the platform 3 , the mass spectrometer 4 is located above the rear of the displacement platform 3 , and the electrospray device 2 is located directly in front of the mass spectrometer 4 . A sample plate 27 for cultivating the target bacterial strain is arranged on the displacement plate 3 . The electrospray device 2 is fixed on the round hole bracket 18; the round hole bracket 18 is fixed on the three-dimensional micro-adjustment base 19, and the front and rear micro-adjustment knobs 20, left and right micro-adjustment Knob 21 and fine adjustment knob 22 up and down. The displacement platform 3 is fixed on the hori...
Embodiment 2
[0035] Embodiment 2: Determination of squalene content in the single colony of marine yeast cultured on the plate
[0036] The agar plate coated with 50 μL of marine yeast liquid was cultured in a 25°C incubator for 24 hours, and a total of 186 single colonies grew out, with a maximum diameter of about 10 mm and an average diameter of about 5 mm; place the plate 15 mm below the laser interface At , set the Nd:YAG solid-state pulsed laser with an energy of 600mJ / cm 2 , the formed laser spot is about 0.5mm. The gas-phase molecular plume of metabolites formed after the laser bombards the colony cells and the external electrospray interface (the voltage applied by the front-end quartz electrospray needle is 5kV, and the inner diameter of the quartz electrospray needle is 50 μm; the outlet of the quartz electrospray needle The inner diameter of the tip is 5 μm; the injection flow rate of the solvent reagent added into the injection needle is 100nL / min), and the high-voltage charge...
Embodiment 4
[0045] Embodiment 4: the detection of squalene content in the chlorella single algal colony cell on the plate
[0046] Incubate the agar plate coated with 80 μL of Schizochytrium bacteria solution in a 33°C incubator for 18 hours, and a total of 173 single colonies grew out, with a maximum diameter of about 8 mm and an average diameter of about 4 mm; place the plate on the laser interface At 10mm below, set the Nd:YAG solid-state pulse laser with an energy of 400mJ / cm 2 , the formed laser spot is about 1mm. The gas-phase molecular plume of metabolites formed after the laser bombards the colony cells and the external electrospray interface (the voltage applied by the front-end quartz electrospray needle is 4kV; the inner diameter of the quartz electrospray needle is 60 μm; the outlet of the quartz electrospray needle The inner diameter of the tip is 15 μm; the injection flow rate of the solvent reagent added into the injection needle is 400nL / min), and the high-voltage charged...
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