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Hydrogel and preparation method thereof

A technology of hydrogel and sodium alginate, applied in medical science, bandages, etc., can solve the problem of difficult uptake of DNA molecules, and achieve the effect of improving cell activity, easy control and good strength

Pending Publication Date: 2020-12-18
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such products are usually directly doped with low-molecular DNA in the emulsion; such free DNA molecules are easily hydrolyzed or degraded by DNA enzymes in the body; at the same time, due to the negative charge of the cell membrane and DNA molecules, DNA molecules are difficult to uptake by cells

Method used

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  • Hydrogel and preparation method thereof

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preparation example Construction

[0041] The embodiment of the present invention provides a method for preparing a hydrogel, which specifically includes the following steps:

[0042] Step 1): Preparation of DNA-loaded calcium carbonate nanoparticles CaCO 3 @DNA.

[0043] This step is specifically: after dissolving low-molecular-weight DNA (1-5mg / mL) in water and stirring evenly, slowly add CaCl 2 Solution (0.1-0.5mol / L), stirred for 0.5-2h. Then add Na dropwise 2 CO 3 Solution (0.1-0.5mol / L), stirred for 12-24h. After the reaction, the precipitate was collected by centrifugation, and the precipitate was freeze-dried to obtain CaCO 3 @DNA.

[0044] Wherein, the molecular weight of the low molecular weight DNA is 25-700bp. The low-molecular-weight DNA is salmon sperm low-molecular-weight DNA (preferably, the DNA used in the specific embodiments of the present invention is a 25-700bp salmon sperm DNA purchased by the inventor). CaCl 2 The concentration of the solution is 0.1-0.5mol / L. Na 2 CO 3 The co...

Embodiment 1

[0054] Embodiment 1 prepares a kind of hydrogel, specifically comprises the following steps:

[0055] Step 1): 20 mg of salmon essence low molecular weight DNA (25-700 bp) was dissolved in 10 mL of deionized water and stirred evenly to obtain an aqueous solution of DNA. 10 mL of CaCl with a concentration of 0.1 mol / L 2 The solution was slowly added dropwise to the DNA solution and stirred for 1 h. Then 10 mL of Na with a concentration of 0.1 mol / L 2 CO 3 The solution was slowly added dropwise to the above solution and stirred for 12 hours. After the reaction was completed, the solution was transferred to a centrifuge tube and centrifuged at 8000r for 20 minutes. Discard the supernatant, collect the precipitate, and freeze-dry the precipitate to obtain CaCO 3 @DNA nanoparticles.

[0056] Step 2): Dissolve 0.5g of sodium alginate in 10mL of deionized water and stir evenly to prepare sodium alginate solution; dissolve 0.5g of chitosan in 10mL of deionized water and stir even...

Embodiment 2

[0063] Embodiment 2 prepares a kind of hydrogel, concrete preparation steps are as follows:

[0064] Step 1) 20 mg of salmon essence low molecular weight DNA (25-700 bp) was dissolved in 10 mL of deionized water and stirred evenly to obtain an aqueous solution of DNA. 10 mL of CaCl with a concentration of 0.1 mol / L 2 The solution was slowly added dropwise to the DNA solution and stirred for 1 h. Then 10 mL of Na with a concentration of 0.25 mol / L 2 CO 3 The solution was slowly added dropwise to the above solution and stirred for 12 hours; after the reaction, the solution was transferred to a centrifuge tube and centrifuged at 8000r for 20 minutes. The supernatant was discarded, and the precipitate was collected; the precipitate was lyophilized to obtain CaCO 3 @DNA nanoparticles.

[0065] Step 2) Dissolve 0.5g sodium alginate in 10mL of deionized water and stir evenly to prepare a sodium alginate solution; dissolve 0.5g chitosan in 10mL of deionized water and stir evenly ...

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Abstract

The invention relates to a hydrogel and a preparation method thereof, and relates to the technical field of medical materials. According to the main technical scheme, the preparation method of the hydrogel comprises the following steps: step 1), preparing calcium carbonate nanoparticle CaCO3 at DNA loaded with DNA; 2) preparing a first reaction solution from sodium alginate, chitosan and CaCO3 atDNA; adding gluconolactone into the first reaction solution and stirring, and obtaining a mixed solution; and standing the mixed solution to obtain the hydrogel. The preparation method is mainly usedfor preparing the hydrogel which can slowly release DNA and is good in structural uniformity; when the hydrogel is applied to a wound sore surface, seepage can be adsorbed, bacteria can be blocked, and a wet environment can be maintained; meanwhile, salt in the body fluid and Ca < 2 + > in the hydrogel are subjected to ion exchange, so that the alginic acid network is slowly degraded, and the calcium carbonate nanoparticles loaded with DNA are released. Compared with free DNA molecules, the calcium carbonate nanoparticles can transfer DNA into cells more efficiently, and proliferation of the cells is promoted.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a hydrogel and a preparation method thereof. Background technique [0002] Acute and chronic wounds can cause severe physical harm to patients and impose a huge socioeconomic burden. A wound dressing is a biological material used to treat skin lesions. Wound dressings can provide a favorable microenvironment for the healing of the wound surface of the skin, and have functions such as hemostasis, prevention of wound infection, and promotion of wound healing. [0003] Hydrogels are considered effective wound dressings. Among them, sodium alginate is a natural polysaccharide, and its molecule is composed of β-D-mannuronic acid (M unit) and α-L-guluronic acid (G unit) connected by (1→4) bonds. , with the stability, solubility, viscosity and safety required for pharmaceutical preparation excipients. Sodium alginate can be combined with divalent cations (such as Ca 2+ 、C...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0023A61L26/0004A61L26/008A61L26/0061A61L26/0066A61L2300/258A61L2300/412
Inventor 胡浩
Owner QINGDAO UNIV
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