Application of Liquidamia hordgsonii Hook. polysaccharide in preparation of drugs for treating and/or preventing liver diseases

Aster and polysaccharide technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of Sichuan Aster research and its insufficient utilization, and achieve the effect of increasing the activity of SOD and CAT, increasing the ratio, and reducing the level of MDA

Inactive Publication Date: 2020-12-18
WUHAN UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to solve the problem of insufficient research and utilization of Aster sichuanensis in the prior art, and aims to provide the application of Aster sichuanensis polysaccharide in the preparation of low-toxicity and high-efficiency liver-protecting drugs

Method used

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  • Application of Liquidamia hordgsonii Hook. polysaccharide in preparation of drugs for treating and/or preventing liver diseases
  • Application of Liquidamia hordgsonii Hook. polysaccharide in preparation of drugs for treating and/or preventing liver diseases
  • Application of Liquidamia hordgsonii Hook. polysaccharide in preparation of drugs for treating and/or preventing liver diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The extraction process of embodiment 1 Aster sichuan polysaccharide

[0041] The crude polysaccharide of Aster sichuan is obtained by drying, pulverizing, water extraction and alcohol precipitation of medicinal materials of Aster sichuan.

[0042]The preparation method of Aster sichuan polysaccharide is as follows: dry and pulverize the Aster sichuan medicinal material, reflux and degrease with 95% ethanol, extract with water at 85°C for 3 hours at a solid-liquid ratio of 1:36, precipitate with 80% ethanol alcohol, and stand overnight at 4°C. Centrifuge (7669g, 10min), collect the precipitate, wash with absolute ethanol and absolute ether respectively, dry under reduced pressure and vacuum to obtain crude polysaccharide; then remove protein by Sevag method and dialyze with deionized water for 3 days (dialysis bag MWCO: 8000Da- 14000Da), with alcohol precipitation, washing and drying in the same way as before, to obtain refined Aster sichuanensis polysaccharide.

Embodiment 2

[0043] Composition analysis of embodiment 2 Aster sichuan polysaccharide

[0044] The Aster sichuan polysaccharide obtained in Example 1 was further separated by Cellufine A-500 cellulose chromatographic column and Toyopearl HW-55F gel chromatographic column. The specific method is as follows: Dissolve the refined polysaccharide of Aster sichuanensis in an appropriate amount of water, add the sample and perform Cellufine A-500 column chromatography (2.0cm×50cm), first elute with water, and then use 0.1mol / L, 0.5mol / L NaCl solution and 2.0mol / L sodium chloride-Tris-HCl (0.05M, pH8.0) gradient elution at a flow rate of 1mL / min, collect 1 tube every 10min, track and detect the sugar content of each tube by phenol-sulfuric acid method, and draw the elution curve . After separation by Cellufine A-500 column chromatography, two main components were obtained, ie 0.1M NaCl eluted component LHP1 with a yield of 68.8%; 0.5M NaCl eluted component LHP2 with a yield of 2.27%. LHP1 was p...

Embodiment 3

[0046] Example 3 Study on the hepatoprotective efficacy of Aster sichuanensis polysaccharide

[0047] 1. Animal grouping, modeling and administration methods

[0048] (1) Carbon tetrachloride modeling

[0049] After SD rats were fed adaptively for 1 week, they were randomly divided into 6 groups (6 rats in each group), i.e. normal control group, model group, high, medium and low administration groups of Aster sichuanensis polysaccharide, positive control group (biphenyl biphenyl esters), all administered by intragastric administration. The rats in the high, medium and low doses of polysaccharide administration groups were given 400mg / kg, 200mg / kg, 100mg / kg Aster polysaccharide per day respectively, the rats in the positive control group were given 100mg / kg bifendate every day, the normal control group Rats in the control group and the model group were given 1 mL / 100 g of distilled water every day, and they ate and drank as usual for 7 consecutive days. 1 hour after the last...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and relates to an application of Liquidamia hordgsonii Hook. polysaccharide in preparation of drugs for treating and / or preventingliver diseases. A rat liver injury model induced by carbon tetrachloride and clivone (endogenous hepatotoxin pyrrolizidine alkaloid) is adopted, the liver protection effect is investigated, and the result shows that the Liquidamia hordgsonii Hook. polysaccharide can significantly reduce the liver function index and the oxidative stress index of a liver injury rat, can be used for research and development of safe and effective liver protection drugs, and especially can be used for prevention and treatment of traditional Chinese medicine-derived liver injury drugs caused by pyrrolizidine alkaloid. The application has important significance for reasonable development and utilization of the Sichuan Chinese herbal medicine Liquidamia hordgsonii Hook.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to the application of Aster sichuanensis polysaccharide in the preparation of therapeutic and / or preventive medicines. Background technique [0002] Drug-induced liver injury (Drug-induced liver injury, DILI) is one of the more common liver diseases in clinical practice. In recent years, with the increase in the types and demands of clinical drugs, the development of new drugs and the wide application of traditional Chinese medicines, the incidence of DILI (especially Chinese herbal medicine-induced liver injury) has also increased. [0003] Sichuan Aster comes from the roots and rhizomes of Ligularia hodgsonii Hook., a plant of the genus Ligularia in the family Asteraceae, and has a long history of folk use as a national medicine or herbal medicine. Sichuan Aster is included in the standards of traditional Chinese medicinal materials in Sichuan Pr...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P1/16C08B37/00A61K36/28A61K125/00
CPCA61K31/715A61K36/28A61P1/16C08B37/0003
Inventor 汤俊文静熊程敏
Owner WUHAN UNIV
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