Multiplex real-time fluorescent RT-PCR primer probe compositions and kits for novel coronavirus nucleic acid testing

Abstract

The invention discloses multiplex real-time fluorescent RT-PCR primer probe compositions and kits for novel coronavirus nucleic acid testing. The kits comprises multiplex real-time fluorescent RT-PCRprimers and probes for testing an ORF1ab gene and an N gene of SARS-CoV-2; the upstream primer body for amplifying the ORF1ab gene is shown as SEQ ID NO: 1, and the downstream primer body for amplifying the ORF1ab gene is shown as SEQ ID NO: 2; a probe sequence for testing the ORF1ab gene is shown as SEQ ID NO: 3; the upstream primer body for amplifying the N gene is shown as SEQ ID NO: 4, and thedownstream primer body for amplifying the N gene is shown as SEQ ID NO: 5; and the probe sequence for testing the N gene is shown as SEQ ID NO: 6. Clinical verification of China and America proves that the kits can meet testing of clinical samples at home and abroad.

Description

technical field [0001] This patent belongs to the field of medical in vitro diagnosis, and relates to a multiple real-time fluorescent RT-PCR primer probe composition and kit for the detection of novel coronavirus nucleic acid. Background technique [0002] All editions of the "Diagnosis and Treatment Plan for Novel Coronavirus Pneumonia" issued by the National Health and Medical Commission (now updated to the eighth trial version) clearly list real-time fluorescent RT-PCR detection of positive nucleic acid of the new coronavirus SARS-CoV-2 as one of the diagnostic criteria First, the timely and accurate identification of SARS-CoV-2 is the prerequisite for reasonable clinical treatment and scientific prevention and control of novel coronavirus pneumonia (COVID-19). [0003] However, at the beginning of the outbreak, the State Drug Administration launched the "Emergency Approval Procedures for Medical Devices" to meet a large number of testing needs. As a result, reagent manu...

AI Technical Summary

Problems solved by technology

At present, most of the commercialized kits that have been put into clinical use only use the Wuhan-Hu-1 virus strain (NC_045512) originally reported in my country as the design template when designing their PCR primers and probes, while SARS-CoV-2 is used as the design template. RNA viruses are prone to mutations, resulting in a certain degree of missed detection

Images