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Portulaca oleracea active component and preparation method and application thereof

An active component, a technology of purslane, which is applied in the field of active components of purslane and its preparation, can solve the problems of many steps in the preparation method, complicated operation and the like, and achieves simple and easy process, good renal protection effect, and good lowering effect. The effect of uric acid efficacy

Active Publication Date: 2020-12-08
BEIHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Preliminary experiments by the inventors have confirmed that amaranth, which is similar to purslane, has the effect of reducing uric acid, but if it is necessary to ensure that the extracted active ingredients have better activity, there are many steps in the preparation method and the defects of complicated operation

Method used

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  • Portulaca oleracea active component and preparation method and application thereof
  • Portulaca oleracea active component and preparation method and application thereof
  • Portulaca oleracea active component and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Research on the preparation method of active components of purslane.

[0042] 1. Experimental method

[0043] Purslane Chinese herbal decoction pieces were purchased from Baokang Chinese herbal decoction pieces processing company, which was confirmed to be purslane by the Department of Pharmacy, Beihua University.

[0044] Weigh 1 kg of dried purslane, add distilled water to make the ratio of solid to liquid 1:12, extract at 65°C, and extract for 1.5 hours. The reflux method was used to extract three times, and each filtrate was combined and centrifuged (8000r / min, 10min), and the precipitate was discarded to obtain a water extract.

[0045] 1.2 Pre-filtration.

[0046] The above water extract is filtered through a 0.45 μm clarification membrane for pretreatment, that is, PO water extract, and macromolecular substances in the solution are removed.

[0047] 1.3 Ultrafiltration.

[0048] Before ultrafiltration, flush the system with 40L deionized water or pure water, ...

Embodiment 2

[0081] The active ingredient of purslane prepared under the optimum conditions in Example 1 was studied for its anti-inflammatory effect.

[0082] 1. Experimental method

[0083] 1.1 Animal feeding conditions

[0084] They were raised in clean cages in the animal room of Beihua University, with an indoor temperature of 22°C±3°C and a humidity of 50%±10%.

[0085] 1.2 Grouping and administration of animals

[0086] Rats were divided into random groups, 10 in each group, respectively blank group (CON), acute gouty arthritis model group (goutyarthritis, GOUT), colchicine tablet group (colchicine, COL, 3.2×10 -4 g / kg / d), high-dose purslane group (PO-H, 2g / kg / d) and low-dose purslane group (PO-L, 1g / kg / d). The drug was administered orally once a day at 8 o'clock in the morning, and the drug was administered continuously for 7 days.

[0087] 1.3 Establishment of acute gouty arthritis model

[0088] The arthritis model was established according to Chen's method. After 7 days of...

Embodiment 3

[0115] The active components of purslane prepared under the optimal conditions of Example 1 were studied on the uric acid-lowering effect.

[0116] 1 Experimental method

[0117] 1.1 Animal feeding conditions

[0118] They were kept in clean cages in the animal room of Beihua University. The indoor temperature is 22℃±3℃, and the humidity is 50%±10%.

[0119] 1.2 Establishment of high uric acid model and administration of animals in groups

[0120] Select 18-22g healthy male mice, and randomly divide them into groups: blank group (CON), hyperuricemia model group (hyperuricemia, HUA), allopurinol group (allopurinol, ALLOP, 0.02g / kg / d), purslane High dose group (PO-H, 1.48g / kg / d); purslane low dose group (PO-L, 0.74g / kg / d). The way of administration is preventive administration, except the CON group and the HUA group, each group is given the corresponding dosage by intragastric administration continuously for 21 days. From the 7th day, except for the CON group, the mice in e...

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Abstract

The invention relates to a portulaca oleracea active component and a preparation method and application thereof, and belongs to the technical field of natural active components. The preparation methodof the portulaca oleracea active component comprises the following steps of performing extraction: taking portulaca oleracea, adding water as a solvent, and performing heating extraction to obtain awater extract solution; and performing ultrafiltration: filtering the water extract solution through an ultrafiltration membrane with the molecular weight cutoff of 5K-15K, and taking a filtrate, so that the portulaca oleracea active component is obtained. The preparation method of the portulaca oleracea active component adopts an ultrafiltration mode for extraction and purification and has the advantages of simple and feasible process, experiments prove that the obtained portulaca oleracea active component has good anti-inflammatory and analgesic effects, and biochemical index detection and pathological analysis of animal experiments prove that the component has a good urate-lowering effect and a good kidney protecting effect. Furthermore, the therapeutic effect of PO on gouty diseases isexplored, and a basic theory and a scientific basis are provided for subsequent research and patent medicines of the PO.

Description

technical field [0001] The invention relates to the technical field of natural active ingredients, in particular to an active ingredient of purslane and its preparation method and application. Background technique [0002] At present, the prevalence of gout and hyperuricemia is increasing day by day, seriously affecting people's normal life. Most of the drugs commonly used in clinical treatment of gouty arthritis and hyperuricemia are western medicines. If taken for a long time, they will cause side effects such as gastrointestinal damage, liver and kidney damage. Therefore, it is imminent to develop drugs that are safe, effective, have few side effects, and can be taken for a long time. [0003] Purslane (Portulaca oleraeea L, PO), also known as Five Elements Grass and Longevity Vegetable, is an annual herb of the family Portulacaceae. The World Health Organization calls PO a "global panacea", and it is also one of the 101 wild plants with the same origin of medicine and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P19/06A61P19/02A61P29/00A61P13/12
CPCA61K36/185A61P19/06A61P19/02A61P29/00A61P13/12
Inventor 王曼力杜培革安丽萍赵南晰郭笑苑广信盛瑜徐广宇李洪宇孙晶波韩笑吕有为
Owner BEIHUA UNIV
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