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Method for the determination of seven non-selective cyclooxygenase inhibitory drug residues in poultry and livestock meat

A non-selective, cyclooxygenase technology, used in measurement devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity and specificity and complexity of high performance liquid chromatography, achieve optimal operating back pressure, improve Resolution, the effect of eliminating matrix effects

Active Publication Date: 2022-07-26
杭州市食品药品检验研究院
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  • Abstract
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Problems solved by technology

Since the sample pretreatment of GC requires derivatization, the pretreatment is more complicated, while the sensitivity and specificity of high performance liquid chromatography are relatively low

Method used

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  • Method for the determination of seven non-selective cyclooxygenase inhibitory drug residues in poultry and livestock meat
  • Method for the determination of seven non-selective cyclooxygenase inhibitory drug residues in poultry and livestock meat
  • Method for the determination of seven non-selective cyclooxygenase inhibitory drug residues in poultry and livestock meat

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Embodiment 1

[0078] Embodiment 1. A dispersed solid-phase extraction purification-ultra-high performance liquid chromatography-tandem mass spectrometry method suitable for seven kinds of non-selective cyclooxygenase inhibitory drug residues in poultry and livestock meat, and the following steps are carried out in sequence:

[0079] 1), prepare the sample solution to be tested:

[0080] (1) Take poultry and livestock meat as the sample to be tested, pulverize it into minced meat with a pulverizer, mix well, and use it as a sample (matrix);

[0081] Poultry meat includes pork, beef, mutton, chicken, duck, etc.

[0082] (2) Weigh above-mentioned sample 5g, accurate to 0.01g, place in polyethylene centrifuge tube, add 5ml 0.2mol / L hydrochloric acid solution, add 5ml 0.02mol / L ascorbic acid solution simultaneously, vortex for 2min;

[0083] (3) 100ul of 0.5mg / ml pepsin was added to the result of step (2), and 37°C constant temperature water bath was shaken for 12h;

[0084](4) 50ml of acetoni...

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Abstract

The invention discloses a method for determining seven kinds of non-selective cyclooxygenase inhibitory drug residues in poultry and livestock meat. Extraction and purification of non-selective COX inhibitory drug residues, using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry with the advantages of high sensitivity and strong anti-interference to detect seven non-selective COX inhibitors in poultry and livestock meat Qualitative and quantitative analysis of drug residues. Seven non-selective cyclooxygenase inhibitors: salicylic acid, ibuprofen, diclofenac sodium, indomethacin, piroxicam, naproxen, phenylbutazone. The invention well solves the problem in the process of detecting seven kinds of non-selective COX inhibitory drug residues in poultry meat.

Description

technical field [0001] The invention relates to a dispersed solid-phase extraction purification-ultra-high performance liquid chromatography-tandem mass spectrometry detection method suitable for seven kinds of non-selective cyclooxygenase inhibitory drug residues in poultry and livestock meat. Background technique [0002] Nonsteroidal anti-inflammatory drugs (NSAIDs) are a large class of non-corticosteroid drugs with anti-inflammatory, analgesic and antipyretic functions, and anti-platelet effects, mainly used for inflammatory immune diseases. Symptomatic treatment can effectively relieve local pain, swelling and other symptoms of muscles, joints and inflammatory immune diseases. Traditional NSAIDs mostly use carboxylic acid groups as active groups. Although they can be divided into phenylpropionic acid, phenylacetic acid, indole, and fenamic acid according to their chemical structures, the carboxylic acid group is mainly related to epoxy. Coxase (COX, including COX-1 and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/32G01N30/30G01N30/16G01N30/60G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/32G01N30/30G01N30/16G01N30/60G01N30/72G01N2030/062G01N2030/146G01N2030/067G01N2030/324
Inventor 励炯刘英吴琼扈明洁林伟杰金朦娜邱红钰
Owner 杭州市食品药品检验研究院
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