Method for screening trimethylamine lyase inhibitor by utilizing microorganisms
A technology of trimethylamine and lyase, applied in the field of microorganisms, can solve the problems of accelerated drug screening, time-consuming and labor-intensive
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[0022] 1) Selection and cultivation of indicator bacteria based on choline conversion to trimethylamine
[0023] After purchasing the indicator bacteria Klebsiella pneumoniae from ATCC (( Klebsiella Pneumoniae )) ATCC 13883, Klebsiella pneumoniae ATCC 13883 was inoculated onto Nutrient Broth (NB) solid plates from a slant and incubated anaerobically at 37°C for 24 hours. Inoculate the activated bacterial species into the test tube containing the NB liquid medium, and incubate at 37 ° C for 24 hours to prepare the indicator bacteria liquid.
[0024] Among them: NB solid medium: each liter of medium contains: peptone 10g, beef extract 3g, sodium chloride 5g, agar 20g, distilled water 1000mL, adjust the pH to 7.2; 121 ℃ autoclave for 15min.
[0025] NB liquid medium: Each liter of medium contains: peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1000mL, pH adjusted to 7.2; autoclaved at 121°C for 15min.
[0026] 2) Determination of minimum inhibitory concentra...
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