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Method for separating and purifying extracellular polymeric substance

An extracellular polymer, separation and purification technology, which is applied in the field of effectively extracting and concentrating the soluble extracellular polymer of Chlorella, can solve the problem of difficulty in obtaining a preset concentration of the soluble extracellular polymer, and achieves a wide range of trials and saves money. Cost, reduction effect

Inactive Publication Date: 2020-11-27
NANKAI UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a method for separating, purifying, concentrating, and preserving soluble extracellular polymers, aiming to solve the problem that there are many extraction methods for existing algae extracellular polymers, but for efficient Technical problems such as purification, enrichment of soluble extracellular polymers, and difficulty in obtaining preset concentrations of soluble extracellular polymers

Method used

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  • Method for separating and purifying extracellular polymeric substance
  • Method for separating and purifying extracellular polymeric substance
  • Method for separating and purifying extracellular polymeric substance

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Embodiment 1

[0035] Chlorella culture: Chlorella algae species (FACHB-8) were added to sterilized BG-11 medium and cultivated in a light incubator at a temperature of 25°C and a light intensity of 30 μmolm -2 the s -1 , the light: dark time ratio is 12h:12h, the absorbance value at 689nm (OD 689 ) Algae growth standard curve prepared, calculate the growth of algae.

[0036] Extraction of soluble extracellular polymer by centrifugation: Centrifuge the collected algae cells at 4000g for 15min at 4°C, take the supernatant and pass it through a 0.45μm filter membrane, and the filtrate is the crude soluble extracellular polymer.

[0037] Purification and Concentration of Soluble Extracellular Polymers: Using figure 2 In the tangential flow ultrafiltration device shown, the crudely extracted soluble extracellular polymer is quantitatively placed in a sample bottle, and concentrated through the tangential flow device, and every 1 L is concentrated to 30 mL. The rotation speed of the peristalt...

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Abstract

The invention relates to the field of algal basic research, and particularly discloses a method for separating and purifying an extracellular polymeric substance. The method comprises the following steps of taking chlorella liquid, performing centrifuging to obtain a supernatant, enabling the supernatant to pass through a 0.45-micron filter membrane to obtain a soluble extracellular polymeric substance, concentrating a filtrate to a small volume by adopting a tangential flow ultrafiltration technology, performing dialyzing, performing freeze-drying to obtain soluble extracellular polymeric substance solid powder, and performing re-dissolution to obtain the soluble extracellular polymeric substance with the preset concentration. The method has the characteristics of simplicity in operation,time and labor saving, easiness in storage, wide trial range and the like.

Description

technical field [0001] The invention relates to the field of basic research on phycology, in particular to a method for effectively extracting concentrated chlorella soluble extracellular polymers. Background technique [0002] As a common substance around algae or bacteria, extracellular polymers are mainly some high molecular polymers secreted by bacteria and algae, including some high molecular substances, such as polysaccharides, proteins and nucleic acids. Extracellular polymers are ubiquitous in natural water bodies, soils and activated sludge aggregates, and have important physiological functions. It plays an important role in sewage treatment and mineral-organic interface research. Moreover, extracellular polymers also account for a considerable part of natural organic matter in natural water bodies. Extracellular polymers play a role in protecting algae or bacteria from external disturbances and play an important role in the field of environmental toxicology resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/34C12N1/12C08B37/00C12R1/89
CPCC07K1/145C07K1/34C12N1/12C08B37/0003
Inventor 周启星曾辉欧阳少虎罗吉伟侯萱
Owner NANKAI UNIV
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