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Stem cell active factor and freeze-dried powder thereof

An active factor, freeze-dried powder technology, applied in skin care preparations, cosmetics, cosmetic preparations, etc., can solve the problems of poor repair effect, loss of cytokines, low practical application value, etc., to reverse the time and repair the skin. Barrier, anti-aging effect

Inactive Publication Date: 2020-11-24
广东先康达细胞库有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide stem cell active factor and its freeze-dried powder, which has the advantages of high application value, and solves the problem that the existing stem cell active factor extraction method only removes some impurities, but the impurities such as sugar are not removed, and the Lead to a large loss of cytokines, poor repair effect, and low practical application value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] (2) Preparation of freeze-dried powder:

[0033] A. Put the stem cell active factor concentrate prepared in the above steps into the reactor for use;

[0034] B. Select 2 to 6 parts of trehalose, 1 to 4 parts of kumquat sugar, 2 to 7 parts of glycine, 2 to 6 parts of arginine, 1 to 4 parts of glycine, 6 to 11 parts of mannitol, 4 parts of tert-butanol ~12 parts, 12~22 parts of honey, mix according to the proportion, mix well with a shaker to make a suspension;

[0035] C. Put the suspension prepared in the above steps into the freezer plate, then put it in the refrigerator at 3~5°C and equilibrate for 45~60min, then control the temperature at -30~-25°C and freeze for 60~80min, then control Freeze at -80~-60°C for 60~90min;

[0036] D. Put 4 to 12 parts of the frozen suspension in the above step into the reaction kettle and mix with the stem cell active factor concentrate, and then put the mixed mixture into a vacuum container, and use the vacuum system to vacuum The pressure ...

Embodiment 1

[0039] First, extract the umbilical cord tissue after stripping the newborn fetus in the delivery room and put it into the umbilical cord transport fluid, and then extract the umbilical cord mesenchymal stem cells for culture after being refrigerated for 24 to 36 hours at 4-6°C. Then, when the cell fusion reaches 65-85 %, gently rinse the cells in the culture flask with physiological saline for 1 to 3 times, add 2 to 6 ml of digestion solution, and place at room temperature for 1 to 3 minutes. Observe that the cells are close to a spherical shape under the microscope, tap the bottle wall gently to stop the digestion, and then transfer to centrifugation Mix the tube, sample and count, and pass through 6000-8000 cells / cm². When the cell density reaches 70-80% in 2 to 3 days, repeat the above operations to obtain a sufficient amount of mesenchymal stem cells. Use P3 to P5 generation Cell preparation, and then when the cell viability is above 80-90%, the flow cytometry markers are q...

Embodiment 2

[0042] In the first embodiment, the following steps are added:

[0043] In the first step, the results of the flow cytometry detection markers show that the cells have the differentiation potential of adipogenesis, bone formation and chondrogenesis.

[0044] First, extract the umbilical cord tissue after stripping the newborn fetus in the delivery room and put it into the umbilical cord transport fluid, and then extract the umbilical cord mesenchymal stem cells for culture after being refrigerated for 24 to 36 hours at 4-6°C. Then, when the cell fusion reaches 65-85 %, gently rinse the cells in the culture flask with physiological saline for 1 to 3 times, add 2 to 6 ml of digestion solution, and place at room temperature for 1 to 3 minutes. Observe that the cells are close to a spherical shape under the microscope, tap the bottle wall gently to stop the digestion, and then transfer to centrifugation Mix the tube, sample and count, and pass through 6000-8000 cells / cm². When the cell...

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Abstract

The invention discloses a stem cell active factor and freeze-dried powder thereof. The stem cell active factor is prepared by the following steps: firstly, extracting umbilical cord tissues from a delivery room after a newborn fetus is peeled off, putting the umbilical cord tissues into umbilical cord transfusion liquid, refrigerating the umbilical cord transfusion liquid in an environment of 4-6DEG C for 24-36 hours, and extracting umbilical cord mesenchymal stem cells for culturing. The stem cell active factor and the freeze-dried powder thereof prepared by the method disclosed by the invention are simple and convenient in preparation method, and the cell source has homogeneity and accords with the biological characteristics of the stem cells, so that the secretion amount and the performance stability of the stem cell source active factor are ensured; and the freeze-dried powder is dissolved by combining moisturizing and water-locking hyaluronic acid and glycerinum, and directly acts on the skin, so that the cell function is activated, the skin is conveniently repaired, the repair effect is good, the use is convenient, the damaged skin can be quickly repaired, the targets of resisting skin aging and repairing skin barrier are achieved, and the effects of maintaining beauty and keeping young, reversing time and retaining youth are achieved.

Description

Technical field [0001] The present invention relates to the technical field of stem cell activity factors, specifically stem cell activity factors and freeze-dried powders thereof. Background technique [0002] In the human body, stem cells are a type of cells that maintain an undifferentiated state, have a high degree of self-renewal and multiple differentiation potentials, and are the progenitor cells of all functional cells in the body. They play an important role in the development of the human body and the maintenance of tissues and organs after maturity. In addition to the ability of stem cells to differentiate into tissue cells to repair tissue damage, a more important regenerative medicine function is paracrine cytokines for tissue repair, such as the secretion of neurotrophic factors, pro-angiogenesis factors, hematopoietic support factors, and immune regulatory factors. , Anti-apoptotic factors, chemokines, etc., but the existing stem cell activity factor extraction met...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/64A61K8/73A61K8/34A61K8/02A61Q19/00A61Q19/08
CPCA61K8/022A61K8/345A61K8/64A61K8/735A61K8/99A61K2800/805A61K2800/84A61Q19/00A61Q19/08
Inventor 陈清法毛文哲杨璐
Owner 广东先康达细胞库有限公司
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