Cerebrospinal fluid exosomal RNA detection device, system and method based on microfluidic technology

Abstract

The disclosure relates to a cerebrospinal fluid exosome RNA detection device, system and method based on microfluidic technology, which belongs to the field of bioengineering technology. The secretion body separation module includes a liquid filtration flow channel; the inlet of the liquid filtration flow channel is connected to the cerebrospinal fluid injection micropump; the cerebrospinal fluid exosome lysis module includes a lysis flow channel; The inlet of the cerebrospinal fluid exosome lysate micropump is also connected; the cerebrospinal fluid detection module includes a cerebrospinal fluid RNA detection pool; several RNA fluorescent probes are arranged in the cerebrospinal fluid RNA detection pool; the entrance of the cerebrospinal fluid RNA detection pool is connected to the outlet of the lysis flow channel; The inlet of the flow channel is also connected with an exonuclease micropump. The device can quickly realize the qualitative analysis of the RNA content, and achieve the purpose of early diagnosis and timely recurrence detection.

Description

technical field [0001] The disclosure belongs to the technical field of bioengineering, and in particular relates to a detection device, system and method for cerebrospinal fluid exosome RNA based on microfluidic technology. Background technique [0002] The statements herein merely provide background information related to the present disclosure and do not necessarily constitute prior art. [0003] Glioma is a neuroectodermal tumor originating from glial cells. It is the tumor with the highest lethal rate in the central nervous system, accounting for about 50% of intracranial tumors. The tumor grows in an obvious infiltrative manner, without obvious boundaries, and is highly invasive. After diagnosis, the 5-year survival rate is less than 10%. Pathological diagnosis is the gold standard to clarify the disease. In addition, computerized tomography (CT) and magnetic resonance scanning (MRI) are important means for glioma diagnosis, staging and typing, and tumor follow-up. U...

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Problems solved by technology

The tumor shows obvious invasive growth, no obvious boundary, and strong invasiveness. After diagnosis, the 5-year survival rate is less than 10%

[0005] The inventors found that: currently, no miRNA and lncRNA detection system based on cerebrospinal fluid exosomes has been proposed and appeared, and its application has filled the gap in glioma body fluid biopsy

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