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Immunoglobulin heavy chain-light chain detection kit and use method thereof

A technology of immunoglobulin and detection kits, which is applied in the field of medical inspection, can solve the problems of cumbersome and strict experimental procedures such as immunofixation, immunoturbidimetric antibody waste, and inability to detect light chain diseases, etc., to achieve more specific binding sites , avoid antibody waste, and reduce the effect of other lesion interfering substances

Pending Publication Date: 2020-11-17
广州市米基医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Kappa type and Lambda type can also be detected for free light chains; the immunoglobulin heavy chain-light chain detection kit of the present invention effectively solves the backward technology of the acetate buffer method in the prior art and the cumbersome and strict experimental process And the problem that the immunofixation method cannot detect light chain disease, and also avoids the problem of antibody waste in the immunoturbidimetric method

Method used

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  • Immunoglobulin heavy chain-light chain detection kit and use method thereof
  • Immunoglobulin heavy chain-light chain detection kit and use method thereof
  • Immunoglobulin heavy chain-light chain detection kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This example provides an immunoglobulin heavy chain-light chain detection kit suitable for electrophoresis, including fixative, heavy chain polyclonal antibody, light chain polyclonal antibody, and Randox quality control serum lyophilized powder.

[0057] Each component of described fixative and the mass percent of each component are as follows:

[0058] trichloroacetic acid 70％ 2-Hydroxy-5-sulfobenzoic acid dihydrate 20％ Phosphate buffer (pH=7.5) 10％

[0059] Described phosphate buffer adopts following raw material preparation:

[0060]

[0061] Each component of the heavy chain polyclonal antibody and the mass percentage of each component are as follows:

[0062]

[0063]

[0064] Each component of the light chain polyclonal antibody and the mass percentage of each component are as follows:

[0065]

[0066] The compound method of described composite indicator:

[0067] (a) Weigh the following reagents and place them in a 10ml ...

Embodiment 2

[0083] This example provides an immunoglobulin heavy chain-light chain detection kit suitable for electrophoresis, including fixative, heavy chain polyclonal antibody, light chain polyclonal antibody, and Bio-Rad quality control serum lyophilized powder.

[0084] Each component of described fixative and the mass percent of each component are as follows:

[0085] trichloroacetic acid 70％ 2-Hydroxy-5-sulfobenzoic acid dihydrate 20％ Phosphate buffer (pH=7.5) 10％

[0086] Described phosphate buffer adopts following raw material preparation:

[0087]

[0088] Each component of the heavy chain polyclonal antibody and the mass percentage of each component are as follows:

[0089]

[0090]

[0091] Each component of the light chain polyclonal antibody and the mass percentage of each component are as follows:

[0092]

[0093] The compound method of described composite indicator:

[0094] (a) Weigh the following reagents and place them in a 10ml c...

Embodiment 3

[0110] This example provides an immunoglobulin heavy chain-light chain detection kit suitable for electrophoresis, including fixative, heavy chain polyclonal antibody, light chain polyclonal antibody, and Randox quality control serum lyophilized powder. Each component of described fixative and the mass percent of each component are as follows:

[0111] trichloroacetic acid 70％ 2-Hydroxy-5-sulfobenzoic acid dihydrate 20％ Phosphate buffer (pH=7.5) 10％

[0112] Described phosphate buffer adopts following raw material preparation:

[0113]

[0114] Each component of the heavy chain polyclonal antibody and the mass percentage of each component are as follows:

[0115] IgG antibody panel Rabbit anti-human IgG polyclonal antibody 40% (target concentration is 4.8g / L) Antibody Stabilizer Tris 59.9％ composite indicator 0.01％ IgA antibody panel Rabbit anti-human IgA polyclonal antibody 40% (target concentration is...

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Abstract

The invention relates to an immunoglobulin heavy-chain light-chain detection kit and a use method thereof, the kit comprises a stationary liquid, a heavy-chain polyclonal antibody and a light-chain polyclonal antibody, and relates to all types of immunoglobulins, combined light chains and free light chains in a human body. The kit not only can detect IgG-[kappa], IgG-[lambda], IgA-[kappa], IgA-[lambda], IgM-[kappa], IgM-[lambda], IgD-[kappa], IgD-[lambda], IgE-[kappa] and IgE-[lambda], but also can detect a Kappa type and a Lambda type. By selecting the multi-site polyclonal antibody capable of being specifically bound, the kit has the advantages of strong specificity, multiple specific binding sites, good affinity, high titer and good rabbit antiserum stability, can greatly improve the detection of different mutation binding sites of immunoglobulin immune dysplasia, can also reduce the influence of other lesion interferents, has high sensitivity, is simple and convenient to operate, and has wide application prospects. The kit is high in detection rate, low in cost, convenient to automate and suitable for clinical detection application.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to an immunoglobulin heavy chain-light chain detection kit and a use method thereof. Background technique [0002] Most of the immunoglobulins in human plasma exist in gamma globulin (gamma-globulin). Can be divided into five classes, namely immunoglobulin G (IgG), immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin D (IgD) and immunoglobulin E (IgE). Among them, IgG is the most important immunoglobulin, accounting for about 70% of human plasma gamma globulin, with a molecular weight of about 150,000 and a sugar content of 2-3. IgG molecules consist of 4 peptide chains. Among them, the peptide chain with a molecular weight of 25,000 is called a light chain, and the peptide chain with a molecular weight of 50,000 is called a heavy chain. The light chain and the heavy chain are connected by a disulfide bond (mdash; Smdash; Smdash). Immunoglobulins ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/531G01N33/53
CPCG01N33/6854G01N33/57407G01N33/57488G01N33/5306G01N33/531
Inventor 陈岡
Owner 广州市米基医疗器械有限公司
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