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Antigen polypeptide for inducing liver cancer specific cytotoxic T lymphocytes and application of antigen polypeptide

A technology of cytotoxicity and lymphocytes, applied in the direction of cancer antigen components, detection of programmed cell death, application, etc., to achieve strong immunogenicity, high positive rate, in vitro killing ability and high efficiency

Active Publication Date: 2020-11-17
深圳市乐土生物医药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on their immunogenicity, let alone whether CTLs can be loaded with these liver cancer-related antigens through antigen-presenting cells such as DCs, and the killing ability of CTLs loaded with these liver cancer antigen polypeptides

Method used

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  • Antigen polypeptide for inducing liver cancer specific cytotoxic T lymphocytes and application of antigen polypeptide
  • Antigen polypeptide for inducing liver cancer specific cytotoxic T lymphocytes and application of antigen polypeptide
  • Antigen polypeptide for inducing liver cancer specific cytotoxic T lymphocytes and application of antigen polypeptide

Examples

Experimental program
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Embodiment

[0085] In this case, a new antigen polypeptide that induces liver cancer-specific cytotoxic T lymphocytes was developed. The development process is as follows: figure 1 As shown, including obtaining whole exome sequencing (Whole exomesequencing) from the TCGA database of liver cancer patients, and performing tumor neoantigen and peptide prediction (Tumorneoantigen), antigen and MHC class I molecular binding affinity analysis (Antigenbinding affinity prediction), screening out pro- New target polypeptide with strong synergistic force; then, antigen-presenting cells are stimulated, and APC cells stimulated by cytokines (IL-4, GM-CSF, IFN-γ and LPS) present antigens to CD8+ T cells (Antigenpresenting) , and stimulate maturation with the corresponding cytokines (IL-21, IL-2, IL-7 and IL-15), CTLs secrete cytokines (IFN-γ) to kill target cells or tumor cells to treat tumor patients.

[0086] The antigenic polypeptide in this example and the CTL induced by it are better than those i...

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Abstract

The invention discloses an antigen polypeptide for inducing liver cancer specific cytotoxic T lymphocytes and application of the antigen polypeptide. The amino acid sequence of the antigen polypeptidefor inducing liver cancer specific cytotoxic T lymphocytes comprises a sequence as shown in Seq ID No. 1. The antigen polypeptide provided by the invention can specifically and efficiently induce liver cancer cytotoxic T lymphocytes, and the liver cancer specific cytotoxic T lymphocytes generated by induction have better performance in immunogenicity, positive rates of antigen-loaded cytotoxic Tlymphocyte cells and killing efficiency in in-vitro cell experiments, compared with CTL induced by other existing antigen polypeptides. The antigen polypeptide is adopted to induce cytotoxic T lymphocytes capable of efficiently and specifically killing liver cancer tumor cells, the preparation method is efficient and convenient, compared with other immune cell therapies, the antigen polypeptide has the characteristics of low cost, high yield and simple technological process, and a new scheme and way are provided for clinical application of liver cancer immune cell therapy.

Description

technical field [0001] The present application relates to the field of liver cancer immune cell therapy, in particular to an antigen polypeptide for inducing liver cancer-specific cytotoxic T lymphocytes and its application. Background technique [0002] Immune cell therapy is the only method that can completely eliminate cancer cells in the existing technology, which makes up for the shortcomings of traditional therapy, and is considered to be the most promising treatment method in the comprehensive tumor treatment model in the 21st century. [0003] Cytotoxic T lymphocytes (abbreviated as CTL) are the most important effector cells against tumors in the body, and are widely used in immune cell therapy. Antigen loading is a necessary process in the preparation of CTL. In the industrialization and clinical application of CTL, it is necessary to solve the specificity and effectiveness of the loaded antigen polypeptide. [0004] At present, in the research and development of C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12N15/12C12N5/0784C12N5/10C12N5/0783A61K35/17A61K39/00A61P35/00A61P1/16C12Q1/02
CPCC07K7/06C12N5/0639C12N5/0636C12N5/0638A61K35/17A61K39/0011A61P35/00A61P1/16G01N33/5011G01N33/505C12N2510/00A61K2039/5154A61K2039/5156A61K2039/5158C12N2503/02G01N2500/10
Inventor 李光磊苏彦景唐超
Owner 深圳市乐土生物医药有限公司
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