A method for rapidly obtaining transgenic plants of capsicum

Abstract

The invention relates to a method for quickly obtaining a capsicum transgenic plant, which comprises the following steps: sampling; activating and infecting agrobacterium; preparing a regeneration culture medium and a screening culture medium; inoculating the immature embryo, and culturing; and forming adventitious bud and regenerating the plant, thereby efficiently obtaining the capsicum transgenic regenerated plant. In the invention, it usually takes 7 weeks from the immature embryo infected by agrobacterium to the acquisition of the regenerated plant, the formation rate of the regenerated plant in the culture process is up to 50% to the maximum, and the positive rate of the transgenic plant is up to 80%. In the invention, the immature embryo is used as a receptor material to infect theagrobacterium and carry out inoculation and culture so as to directly form the regenerated plant, thereby shortening the culture period and enhancing the culture effect and genetic transformation efficiency. If applied to genetic functional verification and breeding practice, the invention can greatly accelerate the research into capsicum functional genomics and the development of breeding practice.

Description

technical field [0001] The invention relates to a method for obtaining transgenic plants in the field of biotechnology, in particular to a method for quickly obtaining transgenic plants by infecting immature embryos of capsicum with Agrobacterium. Background technique [0002] Transgenic technology is the use of recombinant DNA, cell tissue culture, or germplasm system transformation technologies to introduce exogenous genes into plant cells or tissues, allowing them to recombine genetic material in a targeted manner, improve plant traits, and cultivate new varieties of high-quality and high-yield crops. Since the birth of the first transgenic plant in 1983, natural plant genetic engineering has begun to improve the genetic traits of plants under artificial control. Compared with conventional breeding methods, it has the following characteristics: 1) It is not restricted by kinship, and can realize the exchange of genetic material among animals, plants and microorganisms, so...

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Problems solved by technology

However, pepper tissue culture has strong genotype specificity, and the regeneration efficiency is not ideal. In most experiments, the regeneration cycle of the plants is long or the differentiation frequency is low, and the growth ability of the adventitious buds is poor, and some even stay in the bud induction stage and die. cannot grow further, which largely limits the research progress of pepper genetic engineering

At present, the research on pepper transgenic work mainly focuses on the establishment of genetic transformation system, but there are still some major problems in the genetic transformation process that limit the application of pepper transgenic technology in actual breeding, such as: long cultivation period, difficult plant regeneration and genetic transformation rate inferior

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