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Application of NSC 23766 in tumor radiotherapy assistance

A technology of NSC23766, 1. NSC23766, applied in NSC23766 in the field of adjuvant tumor radiotherapy, can solve the problems of limited patient prognosis, high local treatment failure rate, cytotoxicity, etc., and achieve the effect of increasing radiotherapy sensitivity

Pending Publication Date: 2020-11-17
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, radiotherapy is still not ideal for the treatment of lung cancer due to the radiation resistance of cancer cells and the severe cytotoxicity to non-tumor cells that can occur at conventional therapeutic doses
After high-dose radiotherapy combined with chemotherapy or surgery, the local treatment failure rate of NSCLC is still high, and drug or radiation resistance is still the main factor limiting the prognosis of patients

Method used

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  • Application of NSC 23766 in tumor radiotherapy assistance
  • Application of NSC 23766 in tumor radiotherapy assistance
  • Application of NSC 23766 in tumor radiotherapy assistance

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0025] Experimental Example 1 Verification that NSC 23766 inhibits the proliferation activity of LLC cells

[0026] Mouse lung cancer cells LLC were incubated with 10 μM, 50 μM, and 100 μM NSC23766 for 2 h, followed by 0 Gy (non-irradiated), 4 Gy and 8 Gy irradiation treatments, and the proliferation activity of LLC cells was detected by CCK-8 after 24 h. The experimental results showed that: ①Inhibition of Rac1 itself can significantly reduce the proliferation activity of LLC cells; ②50μM and 100μM administration groups can significantly reduce the cell proliferation activity of LLC after different doses of irradiation, that is, the damage effect of sensitized irradiation on LLC cells.

[0027] Such as figure 1 As shown, different doses of Rac1 inhibitor NSC23766 were detected on the proliferation activity of LLC cell line CCK-8. *** represents the corresponding group compared with the PBS control group under the same irradiation dose, P<0.001. The error value adopts mean±S...

experiment example 2

[0028] Experimental Example 2 Verification that NSC 23766 promotes apoptosis of mouse lung cancer cells after irradiation

[0029] Mouse lung cancer cells LLC were incubated with PBS or 100μM NSC23766 for 2h, 0Gy (non-irradiated) or 10Gy irradiated, and 24h later, cell apoptosis was detected by flow cytometry Annexin V / PI method. The experimental results showed that: ①Under the condition of 0Gy without irradiation, there was no significant difference in the apoptosis rate of LLC cells between the administration group and the control group; ②After 10Gy irradiation, the apoptosis rate of each group increased significantly; ③After 10Gy irradiation, the apoptosis rate of the administration group was significantly Higher than the control group (P<0.05). The above results indicated that irradiation induced the apoptosis of mouse lung cancer cells LLC, and NSC 23766 could increase the apoptosis of LLC cells after irradiation, that is, there was a certain sensitization effect on the r...

experiment example 3

[0031] Experimental example 3 Animal experiments

[0032] A total of 33 C57BL / 6 male mice aged 6-8 weeks were selected. After the tumor cells were digested with trypsin containing EDTA, the culture medium was added to resuspend and the cells were counted. Dilute the cell suspension to 4 x 10 7 cells / ml. Medium and Matrigel 1:1, each mouse was injected with 50ul into the right lung through the incision under the right armpit, that is, the number of tumor-bearing cells in the lung of each mouse was 1×10 6 indivual. One week after the lung tumor was loaded, three animals were sacrificed by cervical dislocation, the tumor on the right side of the lung was removed, and the size of the lung tumor was measured by weighing method. The photos and weight of the tumor are as follows: image 3 shown.

[0033] From the second week, the lung tumor-bearing mice were randomly divided into two groups: intraperitoneal injection of PBS group and intraperitoneal injection of 3 mg / kg NSC23766...

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Abstract

The invention relates to the field of biological medicine, in particular to application of NSC 23766 in tumor radiotherapy assistance. The application has the beneficial effect that the NSC 23766 is discovered and proved to increase the radiotherapy sensitivity of tumors for the first time.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of NSC 23766 in adjuvant tumor radiotherapy. Background technique [0002] Lung cancer is a tumor with a high incidence worldwide. In China, lung cancer-related mortality and morbidity are increasing year by year. The most common type of lung cancer is non-small cell lung cancers (NSCLCs), accounting for 85% of human lung cancers, and its common treatment is radiation therapy (RT). However, radiotherapy is still not ideal for the treatment of lung cancer because of the radiation resistance of cancer cells and the severe cytotoxicity to non-tumor cells that can occur at conventional therapeutic doses. After high-dose radiotherapy combined with chemotherapy or surgery, the local treatment failure rate of NSCLC is still high, and drug or radiation resistance is still the main factor limiting the prognosis of patients. In recent years, chemotherapy and radiotherapy for lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/506A61P35/00
CPCA61K31/506A61P35/00
Inventor 沙治林刘虎刘入菱袁红斌
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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