HE staining method and dyeing device

Abstract

The invention relates to the technical field of tissue cell staining, and discloses an HE staining method. The HE staining method comprises the steps of dehydrating, degreasing and enriching a biological material, carrying out hematoxylin staining, cleaning, bluing, carrying out eosin staining to complete HE staining, and dehydrating the biological material by using ethanol solutions with different concentrations, thereby enhancing the dehydration efficiency. Bluing can enable cell nucleus to present obvious blue. The eosin dye liquor can dye the cytoplasm red, so that the comparison betweenthe cytoplasm and the cell nucleus is more distinct, and the HE staining effect is improved. The invention further discloses an HE staining device which comprises a sample preparation assembly, a plurality of solution bottles and a peristaltic pump, a solution is added into or pumped out of the sample preparation assembly through the peristaltic pump, so that the working efficiency is improved; and the sample preparation assembly comprises a test tube, a water bath kettle and an ultrasonic transducer, and can realize water bath heating and uniform oscillation.

Description

technical field [0001] The invention relates to a method for staining tissue sections, in particular to an HE staining method and a staining device. Background technique [0002] In the field of medical biomorphology research, it is usually necessary to stain the morphological structures such as organelles in biological tissues to facilitate visual observation and further inspection. In order to achieve a more favorable visual observation effect, the staining technique for biological tissue is constantly developing, and HE staining technique is usually used, H refers to hematoxylin (Hemaltoxylm), E refers to eosin (Eosin). [0003] Hematoxylin is one of the earliest natural dyes used in biology. It is the most commonly used dye in biomorphology laboratories. It is extracted from the heartwood of the hematoxylin tree. It is in the form of a light yellow-brown powder and is soluble than alcohol and hot water. In the chemical structure of eosin, its acidic coloring groups are...

AI Technical Summary

Problems solved by technology

However, this method also has certain disadvantages. The dyeing solution has many steps and is not easy to master. After mordant dyeing→hematoxylin staining solution dyeing→color separation→reduction→water washing→eosin staining solution dyeing and other processes

[0005] Whether it is the traditional paraffin method or the whole block HE dyeing method, the dyeing process is more complicated, there are many dyeing steps, it is difficult to master, and the yield in actual operation is low

Therefore, it is far from meeting the needs of scientific research work.

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