Root cuttage seedling-raising method of tilia miqueliana
A linden root and root tip technology is applied in the field of improving Nanjing linden root cutting and seedling cultivation, which can solve the problems of low plumpness of Nanjing linden seeds, low value-added coefficient, and few wild resources, etc., so as to improve the ear formation rate, promote division and differentiation, The effect of improving the survival rate
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Embodiment 1
[0025] A kind of Nanjing linden root transplanting seedling raising method comprises the following steps:
[0026] Step 1: Preparation of roots and plants. Select Nanjing Tilia that has grown for 3 years and has no pests and diseases. In autumn, dig out the roots and plants.
[0027] Step 2: root strain treatment, trim the root strain wrapped in step 1 so that the length of the root strain is 25cm, the thickness of the root head is 0.8cm, use 0.5% potassium permanganate solution to clean 3min, and use sterile water rinse repeatedly;
[0028] Step 3: Pre-insertion treatment, soak the roots rinsed in step 2 in the plant hormone solution for 6-8 hours and then take them out;
[0029] Step 4: Hormone treatment, take out the roots soaked in step 3, and dip the bottom of the roots with 5-15mg / L auxin;
[0030] Step 5: root cutting treatment, insert the root plant dipped in auxin in step 4 into perlite with 1-3mm particles, and spray the perlite with 1-3mm particles with 0.5% potas...
Embodiment 2
[0039] A kind of Nanjing linden root transplanting seedling raising method comprises the following steps:
[0040] Step 1: Preparation of roots and plants. Select Nanjing Tilia that has grown for 3 years and has no pests and diseases. In autumn, dig out the roots and plants.
[0041] Step 2: treatment of roots, pruning the roots wrapped in step 1, so that the length of the roots is 15-25cm, the thickness of the roots is 0.6-0.8cm, using 0.5% potassium permanganate solution to clean for 3min, and then Rinse repeatedly with sterile water;
[0042] Step 3: Pre-insertion treatment, soak the roots rinsed in step 2 in the plant hormone solution for 6-8 hours and then take them out;
[0043] Step 4: Hormone treatment, take out the roots soaked in step 3, and dip the bottom of the roots with 5-15mg / L auxin;
[0044] Step 5: root cutting treatment, insert the root plant dipped in auxin in step 4 into perlite with 1-3mm particles, and spray the perlite with 1-3mm particles with 0.5% p...
Embodiment 3
[0053] A kind of Nanjing linden root transplanting seedling raising method comprises the following steps:
[0054] Step 1: Preparation of roots and plants. Select Nanjing Tilia that has grown for 3 years and has no pests and diseases. In autumn, dig out the roots and plants.
[0055] Step 2: treatment of roots, pruning the roots wrapped in step 1, so that the length of the roots is 15-25cm, the thickness of the roots is 0.6-0.8cm, using 0.5% potassium permanganate solution to clean for 3min, and then Rinse repeatedly with sterile water;
[0056] Step 3: Pre-insertion treatment, soak the roots rinsed in step 2 in the plant hormone solution for 6-8 hours and then take them out;
[0057] Step 4: Hormone treatment, take out the roots soaked in step 3, and dip the bottom of the roots with 5-15mg / L auxin;
[0058] Step 5: root cutting treatment, insert the root plant dipped in auxin in step 4 into perlite with 1-3mm particles, and spray the perlite with 1-3mm particles with 0.5% p...
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