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Chitosan-cellulose sulfate magnetic porous composite microsphere as well as preparation and application thereof

A technology of cellulose sulfate and porous composite microspheres, which is applied to the preparation method of peptides, microcapsules, alkali metal compounds, etc., can solve the problems of low mechanical strength, poor solubility of cellulose, limited adsorption capacity, etc., and the method is simple. , Improve solubility and good biocompatibility

Inactive Publication Date: 2020-11-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problems of chitosan being dissolved in acid, low mechanical strength, poor solubility of cellulose, and limited adsorption capacity, and at the same time broadens the application fields of chitosan-cellulose sulfate composite microspheres, such as protein adsorption separation, protein drug delivery and enzyme immobilization etc.

Method used

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  • Chitosan-cellulose sulfate magnetic porous composite microsphere as well as preparation and application thereof
  • Chitosan-cellulose sulfate magnetic porous composite microsphere as well as preparation and application thereof
  • Chitosan-cellulose sulfate magnetic porous composite microsphere as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] At the same time, chitosan and cellulose sulfate were added to 10 mL of 1-ethyl-3-methylimidazole acetate ([Emim] In Ac), under the condition of 80°C oil bath, magnetically stirred and dissolved at a speed of 250r / min for 1h, and a mixed solution of chitosan-cellulose sulfate-[Emim]Ac with a concentration of 3% (g / mL) was prepared ; Subsequently, the nano-Fe 3 o 4 Particles are added to the mixed solution, nano-Fe 3 o 4 The mass volume ratio of the mixed solution is 0.2g: 1mL, magnetically stirred at a speed of 500r / min for 30min to make it uniformly dispersed to obtain a magnetic suspension; then, the magnetic suspension (water phase) is dispersed in a volume ratio of 1:1: 1 In the emulsification system (oil phase) that is made up of liquid paraffin, Span-80 and sodium sulfate aqueous solution (the concentration of aqueous solution is 0.1g / mL), the water-oil ratio (the volume ratio of water phase and oil phase) is 1: 2. Stir magnetically in a 90°C oil bath at a spe...

Embodiment 2

[0051] At the same time, chitosan and cellulose sulfate were added to 10 mL of 1-ethyl-3-methylimidazole acetate ([Emim]Ac) at a mass ratio of 1:1, and at 90°C in an oil bath, With magnetic stirring and dissolving at a speed of 250r / min for 2h, a mixed solution of chitosan-cellulose sulfate-[Emim]Ac with a concentration of 2% was prepared; subsequently, the nano-Fe 3 o 4 Particles are added to the mixed solution, nano-Fe 3 o 4 The mass volume ratio to the mixed solution is 0.2g:1mL, and magnetically stirred at a speed of 500r / min for 1h to disperse evenly to obtain a magnetic suspension. Then, the magnetic suspension (water phase) was dispersed in an emulsification system (oil phase) composed of liquid paraffin, Span-80 and aqueous sodium sulfate (the concentration of the aqueous solution was 0.2 g / mL) at a volume ratio of 1:1:1. ), the water-to-oil ratio was 1:4, and magnetically stirred in a 90°C oil bath at a speed of 1000r / min for 1h; then, twice the volume of absolute ...

Embodiment 3

[0055] At the same time, chitosan and cellulose sulfate were added to 10 mL of 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) at a mass ratio of 4:1, and at 100 °C in an oil bath, With magnetic stirring and dissolving at a speed of 250r / min for 3h, a mixed solution of chitosan-cellulose sulfate-[Emim]Ac with a concentration of 5% was prepared; subsequently, the nano-Fe 3 o 4 Particles are added to the mixed solution, nano-Fe 3 o 4 The mass volume ratio of the mixed solution is 0.2g: 1mL, magnetically stirred at a speed of 500r / min for 3h to make it uniformly dispersed to obtain a magnetic suspension; then, the magnetic suspension (water phase) is dispersed in a volume ratio of 1:1: 1 In the emulsification system (oil phase) composed of liquid paraffin, Span-80 and aqueous sodium sulfate solution (the concentration of the aqueous solution is 0.3g / mL), the water-oil ratio is 1:6, and the rotation speed is 1000r / min at 90 ℃ in an oil bath with magnetic stirring for 1 h; then a...

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Abstract

The invention belongs to the technical field of porous microspheres, and discloses a chitosan cellulose sulfate magnetic porous composite microsphere as well as preparation and application thereof. The preparation method comprises the following steps: 1) dissolving chitosan and cellulose sulfate in an ionic liquid to obtain a mixed solution; 2) dispersing magnetic particles in the mixed solution to obtain a magnetic suspension; mixing the oil phase, an emulsifying agent and a pore-foaming agent to obtain an emulsifying system; and 3) dispersing the magnetic suspension in an emulsification system, emulsifying under a heating condition, carrying out alcohol precipitation, cooling, and carrying out subsequent treatment to obtain the chitosan cellulose sulfate magnetic porous composite microsphere. The method disclosed by the invention is simple, mild in condition, green and environment-friendly. The prepared magnetic porous composite microspheres are rich in pores, large in specific surface area, multiple in active sites and good in lipase immobilization effect. The magnetic porous composite microsphere is applied to enzyme immobilization, protein adsorption separation and protein drug delivery.

Description

technical field [0001] The invention belongs to the technical field of porous microspheres, and in particular relates to a chitosan-cellulose sulfate magnetic porous composite microsphere and its preparation and application. The application of the porous composite microsphere in immobilized lipase, protein adsorption and separation, and protein drug delivery. Background technique [0002] With the rapid development of micron technology, porous microspheres are widely used as enzyme immobilization carriers. Compared with traditional microspheres, porous microspheres have an interconnected inner and outer pore structure, resulting in their lower mass density and larger specific surface area, giving them excellent adsorption capacity. More importantly, the use of porous microspheres composed of natural polymer materials is an effective way to achieve green chemistry and environmental friendliness. [0003] Chitosan is a natural polycationic polymer obtained by deacetylation o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08L1/16C08K3/22C08J9/28B01J20/24B01J20/28B01J20/30C12N11/10C12N11/12C07K1/22A61K9/50A61K47/36A61K47/38
CPCA61K9/5036A61K9/5042B01J20/24B01J20/28009B01J20/28011B01J20/28021C07K1/22C08J9/0061C08J9/008C08J9/28C08J2301/16C08J2305/08C08J2401/16C08J2405/08C08K2003/2275C08K2201/01C08K2201/011C12N11/10C12N11/12
Inventor 王兆梅李雯倩李满凤
Owner SOUTH CHINA UNIV OF TECH
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