Microscopic image-based gramineous plant leaf stomatal index measurement method

Abstract

The invention relates to the technical field of computer vision and deep learning, and provides a microscopic image-based gramineous plant leaf stomatal index measurement method, which comprises the following steps: 1, acquiring a microscopic image of a gramineous plant leaf to be measured; 2, constructing and training an air hole recognition model based on a deep learning target detection algorithm; 3, constructing and training a cell network prediction model based on a deep learning semantic segmentation algorithm; 4, obtaining the total number of air holes by utilizing the air hole recognition model, utilizing the cell network prediction model to obtain a cell network prediction image, sequentially carrying out self-adaptive threshold binarization processing, skeleton extraction, morphological operation of firstly corroding and then expanding, connected domain counting and connected domain filtering on the cell network prediction image to obtain the number of cells, and calculatingstomatal indices. According to the invention, the precision and efficiency of stomatal index measurement can be improved, and the technical problem of misjudgment of stomata and cells due to the timeand labor waste and subjectivity in the prior art is effectively solved.

Description

technical field [0001] The invention relates to the technical fields of computer vision and deep learning, in particular to a method for measuring the stomatal index of gramineous plant leaves based on microscopic images. Background technique [0002] The stomata of plants are the main outlet for water vapor from the body to the outside during the transpiration process, and also the channel for photosynthesis and respiration to exchange with the outside air, thus affecting the processes of transpiration, photosynthesis, respiration, etc. Therefore, the measurement of stomatal index is very important for research. The growth of plants has certain practical significance. [0003] The stomatal index refers to the ratio of the number of stomata per unit area to the total number of epidermal cells and stomata. To obtain the stomatal index, the number of stomata and the number of cells need to be calculated. However, most of the current research is still in the stage of manual cou...

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Problems solved by technology

[0004] Aiming at the problems existing in the prior art, the present invention provides a method for measuring the stomatal index of gramineous plant leaves based on microscopic images, which can improve the accuracy and efficiency of stomatal index measurement, and effectively solve the time-consuming and labor-intensive problems existing in the existing manual counting methods. Subjectivity leads to technical problems with misidentification of stomata and cells

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