Epithelial cell culture medium

Abstract

The invention discloses an epithelial cell culture medium, which comprises the following components: DMEM 13.4g / L, fetal bovine serum 5%, baicalin 10-30mg / L, resveratrol 1-4.5mg / L, glutathione Glycerin 300-500mg / L, penicillin 60mg / mL and streptomycin 100mg / L. The invention reduces the external stimuli during the culture process of the cells, increases the proliferation speed of the cells, and obtains the required number of cells more quickly.

Description

technical field [0001] The invention relates to an epithelial cell culture fluid. Background technique [0002] Cell culture refers to a method of artificially simulating the internal environment in vitro to enable it to survive, grow, reproduce and maintain its main structure and function. Cell culture medium is the material basis for providing nutrition and ensuring the normal growth of cells. The in vitro culture of cells is widely used in the fields of biotechnology and medicine, and is of great significance for the research of human and animal diseases, the development of drugs and even the development of new foods. [0003] At present, the commonly used cell culture medium only adds fetal bovine serum and antibiotics on the basis of DMEM basal culture medium. Some cell lines have poor reproductive ability, poor resistance to environmental changes, and are prone to apoptosis or even necrosis. This results in a long experiment period and low experiment efficiency, whic...

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used cell culture medium only adds fetal bovine serum and antibiotics on the basis of DMEM basic culture medium. Some cell lines have poor reproductive ability, poor resistance to environmental changes, and are prone to apoptosis or even necrosis.

It leads to a long experiment cycle and low experiment efficiency, which brings inconvenience to the research based on cell culture in vitro

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