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C-glycosyl transferase and application thereof in synthesis of schaftoside and isoschaftoside

A technology of glycosyltransferase and sugar disaccharide carbon glycoside compound, which is applied in the field of molecular biology and can solve the problems of unclear biosynthesis process and the like

Active Publication Date: 2020-10-23
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biosynthetic processes of flavonoid dicarbosides with different glycosyl groups, such as schaftosides and isoschaftosides, are still unclear

Method used

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  • C-glycosyl transferase and application thereof in synthesis of schaftoside and isoschaftoside
  • C-glycosyl transferase and application thereof in synthesis of schaftoside and isoschaftoside
  • C-glycosyl transferase and application thereof in synthesis of schaftoside and isoschaftoside

Examples

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Embodiment 1

[0045] Example 1: Cloning, prokaryotic expression, enzyme protein purification and functional characterization of C-glycosyltransferases from Scutellaria baicalensis, corn, rice, licorice, scallop, Zipingia sagegensis, and Yishannanxing

[0046] 1. Screening of C-glycosyltransferase genes in Scutellaria baicalensis, corn, rice, licorice, scallion, Ziping shaogen, and Nanxing

[0047] Download transcriptome data of Scutellaria baicalensis (SRR3123399-RNA, SRR3130396-RNA, SRR3727161-RNA, SRR367956-root, SRR3367955-leaf), licorice transcriptome data (DRR006519, DRR006520, DRR006521, DRR006522, DRR1, 6525, 6SR95, SRR0) from NCBI SRR1783600、SRR1783602、SRR1811619、SRR2537378)、少根紫萍转录组数据(SRR647050、SRR647053、SRR948161、SRR2045955、SRR2045956、SRR2045957、SRR2045958、SRR2048534、SRR2048535、SRR2048536、SRR2048537、SRR2048538)。 Rice and maize use online transcriptome databases. Daxue and Yishan Nanxing performed transcriptome sequencing.

[0048] For the transcriptome data downloaded from NCBI, ...

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Abstract

The invention discloses C-glycosyl transferase and application thereof in synthesis of schaftoside and isoschaftoside. 2-hydroxyflavanone C-glycosyl transferase genes (SbCGTa, GuCGTa, PsCGTa, LpCGTa and AeCGTa) and C-glucose-2-hydroxyflavanone C-glycosyl transferase genes (SbCGTb, ZmCGTb, OsCGTb1, OsCGTb2, GuCGTb, PsCGTb, LpCGTb and AeCGTb) are separated from scutellaria baicalensis, corn, rice, licorice, pistia stratiotes, spirodela oligorrhiza and arisaema erubescens. The invention finds for the first time that proteins coded by the genes participate in synthesis of phytoalexin schaftoside and isoschaftoside in plants, so that a feasible method is provided for synthesizing the schaftoside, the isoschaftoside and analogues thereof by utilizing enzyme catalysis in vitro.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a C-glycosyltransferase (CGT) gene and its coded protein and the application in synthesizing schaftoside and iso-schaftoside. Background technique [0002] Schaftosides and isoschaftosides are a class of flavonoid dicarbosides with different glycosyl groups. As phytoalexins, they are distributed in a variety of plants. Currently, it is contained in 39 species of plants in 19 families, including aquatic and terrestrial plants, monocotyledonous and dicotyledonous plants, and herbaceous and shrub plants. 1 . Schaftosides and isoschaftosides have obvious physiological and pharmacological activities. In golden thread grass, schaftoside acts as an isotropic substance, which can inhibit the growth of Striga 2a-c . In the phloem of rice, schaftosides act as antifeedants. Schaftosides and isoschafutasides in tubers of Artemisia annua have nematicidal activity. In addition, schafutas...

Claims

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Application Information

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IPC IPC(8): C12P19/44C12P19/60C12N9/10C12N15/54
CPCC12P19/44C12P19/60C12N9/1048
Inventor 乔雪叶敏王子龙王双陈宽
Owner PEKING UNIV
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