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A kind of hydroxyapatite composite material with antibacterial function and its preparation method and application

A technology of hydroxyapatite and composite materials, applied in medical preparations containing active ingredients, medical preparations with non-active ingredients, applications, etc., can solve the problems that the research on iodine-modified hydroxyapatite has not been reported, etc. Achieve good regenerative repair effect and improve biocompatibility

Active Publication Date: 2022-02-25
SHANGHAI JIHUO MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Iodine is one of the essential trace elements for humans and animals. It is safe, reliable, broad-spectrum and highly efficient when used in disinfection and sterilization. However, there is no report on the study of iodine-modified hydroxyapatite.

Method used

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  • A kind of hydroxyapatite composite material with antibacterial function and its preparation method and application
  • A kind of hydroxyapatite composite material with antibacterial function and its preparation method and application
  • A kind of hydroxyapatite composite material with antibacterial function and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] (1) Hydroxyapatite is purchased or prepared by chemical precipitation. The preparation method is: weigh 13.875g of anhydrous calcium chloride and dissolve it in 750mL of water, adjust the pH to 9.5 with ammonia water, stir for 30min, and add 250mL of 0.3 M(NH 4 ) 2 HPO 4 Aqueous solution, the pH of the solution is controlled to be 9-10 during the dropping process, then stirred and reacted at 75°C for 12-24 hours, centrifuged, washed alternately with water and ethanol for several times, and dried to obtain the following: figure 1 Hydroxyapatite shown.

[0075] (2) Ultrasonic disperse 5g of hydroxyapatite in 100mL of ethanol, add ammonia water to adjust the pH to 10, add 3g of 3-aminopropyltriethoxysilane, stir at 300rpm and reflux at 75°C for 24h, centrifuge, wash with ethanol Washed twice, dried in vacuum to obtain amino-functionalized hydroxyapatite.

[0076] (3) Dissolve 10g of 6-O-carboxymethyl chitosan (average weight-average molecular weight of 150,000g / mol, de...

Embodiment 2

[0084]Repeat steps (1)(2)(3) in Example 1 to prepare amino-functionalized hydroxyapatite and quaternized carboxymethyl chitosan, the difference is only to select the 6- O-carboxymethyl chitosan (deacetylation degree ≥ 85%, substitution degree ≥ 80%).

[0085] (4) Ultrasonic disperse 1g of amino-functionalized hydroxyapatite in 130mL of water, add 0.65g of quaternized carboxymethyl chitosan to completely dissolve, mix well, add 6.85mL of 100 / 50mM EDC / NHS mixture drop by drop Add in the above solution, the final concentration is 5.0 / 2.5mM, cross-link at room temperature for 24h, the cross-linked solution is centrifuged and washed, and freeze-dried to obtain the surface grafted quaternized carboxymethyl chitosan material (HA -g-GCMC).

[0086] (5) Immerse the obtained HA-g-GCMC in 50mL of 0.01g / mL iodine / ethanol / water (volume ratio of ethanol to water is 1:3), stir at room temperature for 5h, wash and freeze-dry to obtain antibacterial hydroxyphosphorus Gray stone composite mat...

Embodiment 3

[0089] Repeat steps (1)(2)(3) in Example 1 to prepare amino functionalized hydroxyapatite and quaternized carboxymethyl chitosan, the difference is only that the selection of average weight average molecular weight is 1000g / mol of 6- O-carboxymethyl chitosan (deacetylation degree ≥ 85%, substitution degree ≥ 80%).

[0090] (4) Ultrasonic disperse 1g of amino-functionalized hydroxyapatite in 300mL of water, add 1.5g of quaternized carboxymethyl chitosan to dissolve completely, mix well, add 15.80mL of 200 / 100mM EDC / NHS mixture drop by drop Add in the above solution, the final concentration is 10.0 / 5.0mM, cross-linking at room temperature for 24h, the cross-linking solution, centrifugal washing, freeze-drying and drying to obtain hydroxyapatite surface grafted quaternized carboxymethyl chitosan material ( HA-g-GCMC).

[0091] (5) Immerse the obtained HA-g-GCMC in 80mL of 0.01g / mL iodine / ethanol / water (volume ratio of ethanol to water is 1:1), stir at room temperature for 5h, wa...

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Abstract

The invention discloses a hydroxyapatite composite material with antibacterial function and its preparation method and application. The hydroxyapatite composite material with antibacterial function uses hydroxyapatite as the core, and the outer surface of the core is uniformly wrapped with quaternary Ammonized carboxymethyl chitosan molecular layer; complex molecular iodine on the quaternized carboxymethyl chitosan molecular layer. The hydroxyapatite composite material with antibacterial function not only enhances the biocompatibility of hydroxyapatite, facilitates the adsorption of cells, but also endows hydroxyapatite with a long-lasting antibacterial function to overcome antibiotic resistance and the toxicity of heavy metals , has the advantages of broad spectrum, high efficiency and good biological safety; it also further prevents the growth of microorganisms and improves the effect of repair treatment. It is expected to have broad application prospects in the fields of biomedical materials and antibacterial daily necessities.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a hydroxyapatite composite material with antibacterial function and its preparation method and application. Background technique [0002] Hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 , HA) is similar to the mineral components of bones and teeth. Due to its unique crystal chemical structure and a variety of special and excellent properties, it has good biological activity, biocompatibility and degradability. It is commonly used Hydroxyapatite plays an increasingly important role in the field of biomedicine as bone tissue engineering repair and regeneration materials, hemostatic dressings, drug / gene delivery carriers, catalysts, ion adsorption / exchangers, photoelectric reagents, etc. However, hydroxyapatite has disadvantages such as easy agglomeration, difficulty in recycling, high material brittleness, and easy microbial growth. [0003] In addition, hydroxyapatite is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/20A61L27/02A61L27/50A61L27/54A61L27/58A61L15/28A61L15/18A61L15/42A61L15/44A61L15/46A61L15/64A61K8/73A61K8/20A61K8/24A61Q11/00A61Q17/00A61Q19/10A61Q19/00A61Q5/02A61K31/722A61K33/18A61K33/42A61P17/02A61P17/10A61P31/00A61P1/02A61P31/04A61K47/02A61K47/36A61K48/00A23L33/16
CPCA61L27/12A61L27/20A61L27/025A61L27/50A61L27/54A61L27/58A61L15/28A61L15/18A61L15/42A61L15/44A61L15/46A61L15/64A61K8/736A61K8/20A61K8/24A61Q11/00A61Q17/005A61Q19/10A61Q19/00A61Q5/02A61K31/722A61K33/18A61K33/42A61P17/02A61P17/10A61P31/00A61P1/02A61P31/04A61K47/02A61K47/36A61K48/0008A23L33/16A61L2300/404A61L2300/602A61L2300/112A61L2300/106A61L2300/236A61L2300/412A61L2300/41A61L2430/02A61L2430/12A61L2430/38A61L2430/24A61L2430/06A61L2300/416A61L2400/04A23V2002/00A61K2300/00C08L5/08A23V2200/312A23V2250/1578A23V2250/1618
Inventor 朱香利
Owner SHANGHAI JIHUO MEDICAL TECH CO LTD
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