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Application of compound AN-329 for inhibiting releasing and diffusing of hantavirus

A technology of AN-329 and hantavirus, applied in antiviral agents, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of severe symptoms, high mortality, and lack of specific and effective therapeutic drugs

Active Publication Date: 2020-10-23
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Hemorrhagic fever with renal syndrome caused by hantavirus has acute onset, severe symptoms, high mortality, and lack of specific and effective therapeutic drugs

Method used

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  • Application of compound AN-329 for inhibiting releasing and diffusing of hantavirus
  • Application of compound AN-329 for inhibiting releasing and diffusing of hantavirus
  • Application of compound AN-329 for inhibiting releasing and diffusing of hantavirus

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Embodiment Construction

[0017] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0018] 1. Screening of key epitope sequences affecting HTNV-related Gn cytoplasmic tail

[0019] (1) Forecast

[0020] First, use bioinformatics technology to design small molecules that can hinder the interaction between Gn and ESCRT, and select 45 small molecule compounds whose structures can enter the active center of ESCRT from the small molecule compound library.

[0021] (2) Screening

[0022] A549 cells were digested and passaged to 6-well cell culture plates. When the cell density reached 60%, HTNV was infected according to MOI=1. 6 hours after infection, cell culture supernatant was added according to different compound concentrations, mixed evenly, and placed in CO at 37°C. 2 The cells were cultured in the incubator for 24 hours, and the A549 cells were digested and passaged to a new 96-well plate on the same day. After 24 hours ...

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Abstract

The invention discloses application of a compound AN-329 for inhibiting releasing and diffusing of a hantavirus. The compound AN-329 can inhibit a mutual function of hantavirus Gn and a host ESCRT (Endosomal sorting complex required for transport), a certain foundation is laid for researching the life cycle of the hantavirus as well as the mutual action mechanism of the virus and the host, and a thought is provided for researching a medicine capable of resisting the hantavirus. An experiment proves that AN-329 post cells are added, an HTNV (hantavirus) antigen quantity in cells subjected to secondary infection by supernatant is obviously lowered, a nucleic acid level of the HTNV in the cells subjected to secondary infection is obviously low, and the small molecule compound AN-329 effectively inhibit the releasing and the diffusing of the hantavirus.

Description

technical field [0001] The invention belongs to the technical field of microbial immunity, and specifically relates to the application of compound AN-329 to inhibit the release and spread of hantavirus. Background technique [0002] HTNV belongs to the family Bunyaviridae and the genus Hantavirus. In 1976, Korean scholar Lee Ho-wang successfully isolated the virus for the first time from the lung tissue of Apodemus spp. in the Hantan River Basin of the country. Taking Hantaan virus HTNV as a typical Hantaan virus for research, there is a certain understanding and research on the process of HTNV entry and intracellular replication. The life cycle of HTNV is divided into adsorption, penetration, genome release, replication and translation, There are five stages of budding release. In the early stage of virus infection, its budding release is very vigorous, which is the key to its expansion of the scope of infection, but how the virus releases progeny viruses has not yet been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/341A61P31/14
CPCA61K31/341A61P31/14Y02A50/30
Inventor 吴兴安应旗康王芳张晓晓刘梓谕董宇航刘蓉蓉
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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