Integrated solid metabonomics metabolite extraction kit

Abstract

The invention discloses an integrated solid metabonomics metabolite extraction kit which comprises a detachable cracking column and a filtering and collecting bottle. The cracking column comprises a cracking column body, an upper cap and a lower cap. An upper opening and a lower opening of the cracking column body are threaded outer rings and are respectively connected with the upper cap and the lower cap. A filter screen is arranged at the bottom of the cracking column body, and HPLC-grade ceramic crushing beads are arranged in the cracking column body. The filtering and collecting bottle comprises a filtering column and a collecting bottle body, the filtering column is detachably embedded in the collecting bottle body, and internal threads capable of being connected with the lower opening of the cracking column body are arranged on an inner ring of an upper opening of the collecting bottle body. The kit is suitable for pretreatment of various solid samples, the possibility of pollution caused by repeated treatment among samples and the possibility of metabolite degradation caused by overlong operation time are avoided, and meanwhile, the metabolite extraction yield is also increased.

Description

technical field [0001] The invention belongs to the field of metabolomics and relates to the extraction of metabolites in metabolomics, in particular to an integrated solid metabolomics metabolite extraction kit. Background technique [0002] Metabolomics refers to the high-throughput detection and data processing of different metabolites in organs and tissues through the analysis of group indicators, to find the relative relationship between metabolites and physiological and pathological changes, and to study the dynamic metabolic changes of the whole body or tissue cell system , especially the method of exploring the characteristics and effects of endogenous metabolism, genetic variation, environmental change and various substances entering the metabolic system. In recent years, with the update of detection instruments, when genomics and proteomics can only tell us what might happen, metabolomics can already tell us what actually happened, so as to provide us with research...

AI Technical Summary

Problems solved by technology

In addition to the subsequent processes of extract extraction, nitrogen blowing and redissolution, the entire operation takes a long time, and each step needs to be processed in an independent 1.5ml microcentrifuge tube, so the degradation loss of metabolites is inevitable

When the sample size is huge, the traditional extraction method will lead to repeated sample processing and lack of efficiency. While increasing the human workload, it also reduces the accuracy of sample pretreatment and the uniformity of sample processing.

In addition, most of the animal and plant samples often encountered in metabolomics tests are immobile solids. Compared with liquid tissues, they have the characteristics of thicker cell walls, tighter cell arrangement, many types of metabolites, and large differences in chemical properties. Metabolomics requires the extraction of as many metabolites as possible, which brings greater challenges to metabolite extraction

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