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A kind of gelatin/gellan gum/hydroxyapatite composite hydrogel and preparation method thereof

A composite hydrogel and hydroxyapatite technology, which can be used in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of unfavorable tissue cell attachment and proliferation, difficulty in complete degradation, and large cell toxicity, and achieve a balance of reduction Improvement of swelling rate and compressive mechanical strength, effect of improving compressive mechanical strength

Active Publication Date: 2022-05-27
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, synthetic polymers are usually accompanied by high cytotoxicity, which is not conducive to the attachment and proliferation of tissue cells, and is not easy to be completely degraded, which poses challenges to the research and application of tissue engineering.

Method used

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  • A kind of gelatin/gellan gum/hydroxyapatite composite hydrogel and preparation method thereof
  • A kind of gelatin/gellan gum/hydroxyapatite composite hydrogel and preparation method thereof
  • A kind of gelatin/gellan gum/hydroxyapatite composite hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Preparation and characterization of oxidized gellan gum

[0057] 1. Preparation method of oxidized gellan gum

[0058] The preparation process of oxidized gellan gum (OG) is as follows:

[0059] (1) Weigh a certain amount of low-acyl gellan gum (GG), disperse it in 200 mL of distilled water at 90 °C, stir for 30 min, and obtain a uniform GG solution, and gradually cool down to 40 °C.

[0060] (2) Add a certain amount of NaIO to the GG solution 4 , Under the condition of avoiding light, control the oxidation temperature and oxidation time, and stir vigorously to obtain the mixed solution of oxidized gellan gum.

[0061] (3) Pour the oxidized gellan gum mixture into a dialysis bag with a molecular weight of 8-14kDa, and use distilled water for 5 days at room temperature to remove residual NaIO 4 With the reaction by-products, the mixed solution after dialysis is suction filtered, the solution obtained by suction filtration is pre-frozen at -20 ℃ for 12 hours,...

Embodiment 2

[0079] Example 2 Preparation and Characterization of Aminated Hydroxyapatite

[0080] 1. Preparation of Aminated Hydroxyapatite

[0081] The preparation process of aminated hydroxyapatite includes the synthesis of hydroxyapatite (Hap) and the process of preparing aminated hydroxyapatite (mHap) from hydroxyapatite. The specific process is as follows:

[0082] (1) Synthesis of hydroxyapatite

[0083] (1) Dissolve calcium nitrate and diammonium hydrogen phosphate in distilled water to obtain 1.2M Ca respectively 2+ Solution, 0.72M HPO 4 2- solution.

[0084] (2) At 40°C, the Ca 2+ The solution was slowly dripped into HPO according to the volume ratio of 1:1 4 2- In the solution, at the same time, the pH of the mixture was stabilized at 10.5 with ammonia water, and the reaction was continued for 2 h.

[0085] (3) The reaction solution was allowed to stand at 50°C for 12 hours, and the white precipitate was collected by centrifugation. The precipitate was washed alternately...

Embodiment 3

[0109] Example 3 Preparation and characterization of gelatin / oxidized gellan hydrogel

[0110] 1. Preparation method of gelatin / oxidized gellan hydrogel

[0111] The preparation process of gelatin / oxidized gellan gum (Gel-OG) hydrogel is as follows:

[0112] (1) Dissolve 1 g of Gel in distilled water at 50 °C to obtain a 20 wt% Gel solution.

[0113] (2) Weigh a certain amount of OG and dissolve it in distilled water at 40°C to obtain OG solutions of 1wt%, 1.5wt%, 2wt%, 2.5wt% and 3wt% respectively; wherein, oxidized gellan gum (OG) Prepared by the optimized conditions of Example 1, namely GG and NaIO in the preparation process of OG 4 The mass ratio was 1:0.9, the oxidation temperature was 40°C, and the oxidation time was 8h.

[0114] (3) OG solution and Gel solution with different concentrations were mixed at 40°C in a mass ratio of 1:1.

[0115](4) Pour the mixture into five glass test tubes, each with an inner diameter of 13 mm and a depth of 100 mm, sealed and placed ...

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Abstract

The invention belongs to the technical field of tissue engineering, and relates to a new material applied to cartilage substitutes, in particular to a gelatin / gellan gum / hydroxyapatite composite hydrogel and a preparation method thereof. The composite hydrogel is made of gelatin, low acyl oxidized gellan gum and aminated hydroxyapatite, wherein the weight ratio of gelatin to low acyl oxidized gellan gum is (10-30):(1-3); The added amount of hydroxyapatite is 0.5%-2% of the total weight of gelatin and oxidized gellan gum. The particle size of the modified mHap is significantly reduced, closer to the nanometer scale, which makes the distribution of mHap in the composite hydrogel more uniform, and the hydrogel network formed after introducing mHap into Gel‑OG is more dense, and the introduction of mHap can promote cell proliferation, making Gel‑OG / mHap have good biocompatibility, degradability and self-healing property, which is beneficial to the Gel‑OG / mHap composite hydrogel in cartilage Applications in the field of tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and relates to a new material applied to a cartilage substitute, in particular to a gelatin / gellan gum / hydroxyapatite composite hydrogel, and a preparation method thereof. Background technique [0002] Cartilage tissue is a highly ordered, fibrous and cell-attached hydrogel-like tissue that mainly contains cartilage tissue cells and extracellular matrix (ECM), without lymphatics, blood vessels and other organs. Cartilage tissue contains about 70% water, 20% collagen and 10% protease, and the tissue is gradually divided into four layers inwards, namely surface, middle, deep and calcified cartilage area, each layer has different physical properties. chemical and biological properties. Cartilage tissue covers the ends of bone joints, and has functions such as load decompression and protection of joints from direct mechanical damage. It is very important to the life activities of animals....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L89/00C08L5/00C08K9/06C08K3/32A61L27/52A61L27/46A61L27/50A61L27/58
CPCC08J3/075A61L27/52A61L27/46A61L27/50A61L27/58C08J2389/00C08J2405/00C08K9/06C08K3/32A61L2400/06A61L2430/06
Inventor 汤克勇王绿阳李萌雅王芳刘捷郑学晶
Owner ZHENGZHOU UNIV
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