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Method for separating trilobatin and phlorizin from Lithocarpus polystachyus Rehd

A technology for trilobatin and phlorizin, which is applied in the field of separation of trilobatin and phloridin, and can solve problems such as unsatisfactory yield of trilobatin, waste of natural resources, and high cost

Active Publication Date: 2020-10-20
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both trelobatin and phloridzin belong to dihydrochalcones, which are the main chemical components in Zhijiang wild sweet tea, and have some physiological activities. There are many studies on their extraction in the prior art
Although the patent has obtained high-purity trilobatin, expensive high-speed countercurrent chromatography is used, and the yield of trilobatin is not satisfactory. The patented method is not suitable for large-scale industrial production of trilobatin. Moreover, the patent does not involve the extraction of another active ingredient in Zhijiang wild sweet tea, that is, phlorizin.
[0008] In addition, in the methods disclosed above, there is still a common technical defect - only one natural active ingredient in Zhijiang wild sweet tea (Duosui Shike) can be obtained, and there is no separation and purification of other natural active ingredients in the raw material , to be used, have caused a waste of natural resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Pulverization and extraction: Get 200kg of dried Zhijiang wild sweet tea leaf raw materials, pulverize to a particle size of 2 to 3mm, put it into an extraction tank, and extract twice with hot water at 98°C (the first extraction heat The water consumption is 2800L, and the extraction time is 3 hours; the hot water consumption for the second extraction is 2000L, and the extraction time is 2 hours), filtered to obtain the extract;

[0042] (2) Flocculation, centrifugation, ultrafiltration: the extract is cooled to room temperature, adding flocculant PAC and PAM (consumption is 2kg, 2kg respectively), flocculation, decanter centrifugation, centrifugation is 2000Da ultrafiltration membrane with molecular weight cut off Filtrate under the condition of a pressure of 0.2Mpa to obtain the ultrafiltration membrane filtrate;

[0043] (3) Macroporous resin adsorption: the ultrafiltration membrane filtrate is passed through the macroporous adsorption resin column with a flow r...

Embodiment 2

[0050] (1) Pulverization and extraction: Get 200kg of dried Zhijiang wild sweet tea leaf raw materials, pulverize to a particle size of 3 to 4mm, put it into an extraction tank, and extract twice with hot water at 90°C (the first extraction heat The water consumption is 3000L, and the extraction time is 3 hours; the hot water consumption for the second extraction is 1600L, and the extraction time is 1 hour), and filtered to obtain the extract;

[0051] (2) Flocculation, centrifugation, and ultrafiltration: cool the extract to room temperature, add flocculants PAC and PAM (2kg and 1.5kg respectively), flocculate, centrifuge on a decanter, and use an ultrafiltration membrane with a molecular weight cut-off of 1500Da for the centrifugate Filtrate under the condition of a pressure of 0.3Mpa to obtain the ultrafiltration membrane filtrate;

[0052] (3) Macroporous resin adsorption: pass the ultrafiltration membrane filtrate through the macroporous adsorption resin column with a flo...

Embodiment 3

[0059] (1) Pulverization and extraction: Take 200kg of dried Zhijiang wild sweet tea leaf raw material, pulverize to a particle size of 4-5mm, put it into an extraction tank, and extract it twice with hot water at 92°C (the hot water extracted for the first time The consumption is 2200L, and the extraction time is 3 hours; the hot water consumption for the second extraction is 1800L, and the extraction time is 1.5 hours), filtered to obtain the extract;

[0060] (2) Flocculation, centrifugation, and ultrafiltration: Cool the extract to room temperature, add flocculants PAC and PAM (1.5kg and 2kg respectively), flocculate, centrifuge on a decanter, and use an ultrafiltration membrane with a molecular weight cut-off of 1000Da for the centrifugate Filtrate under the condition of a pressure of 0.35Mpa to obtain the ultrafiltration membrane filtrate;

[0061] (3) Macroporous resin adsorption: the ultrafiltration membrane filtrate is passed through the macroporous adsorption resin c...

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Abstract

The invention provides a method for separating trilobatin and phlorizin from Lithocarpus polystachyus Rehd. The method comprises the following steps: (1) carrying out crushing and extracting; (2) carrying out flocculation, centrifugation and ultrafiltration; (3) carrying out macroporous resin adsorption; (4) removing impurities; (5) carrying out gradient elution: eluting the macroporous adsorptionresin column with low-concentration alcohol and high-concentration alcohol in sequence, and collecting a low-concentration alcohol eluent and a high-concentration alcohol eluent respectively, the low-concentration alcohol containing a small amount of carboxylic acid; (6) carrying out separating and refining of trilobatin; and (7) carrying out separating and refining of phlorizin. According to themethod, two high-content Lithocarpus polystachyus Rehd natural active ingredients can be obtained at the same time, comprehensive utilization of resources is achieved, the process is coherent, operability is high, the equipment requirement is low, the production cost is low, and the method is suitable for industrial production; and the purity and the yield of the obtained trilobatin refined product and phlorizin refined product are ensured and improved.

Description

technical field [0001] The invention relates to a method for natural active ingredients of Zhijiang wild sweet tea, in particular to a method for separating trilobatin and phlorizin from Zhijiang wild sweet tea. Background technique [0002] Zhijiang wild sweet tea, scientific name "Lithocarpus polystachyus Rehd", is a plant belonging to Fagaceae, an evergreen tree, which grows in the dense forest in a wild state, and integrates the three functions of medicine, sugar and tea. [0003] The roots, leaves and fruits of Zhijiang wild sweet tea can be used as medicine, which has the functions of clearing heat and detoxifying, damp-heat dysentery, stopping bleeding and promoting blood circulation, diuretic, regulating endocrine, lowering blood pressure, lowering blood fat, lowering blood sugar, dredging and softening cardiovascular and cerebrovascular, and anti-tumor. Zhijiang wild sweet tea is sugar-free and self-sweetening, with a fresh and mellow taste. Because Zhijiang wild s...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 李伟黄华学
Owner HUNAN HUACHENG BIOTECH
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