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Gastric cancer extremely-early cell marker, gastric cancer precancerous lesion early cell marker and application of markers in diagnostic kits

A very early, kit-based technology, applied in the field of cell markers and its assay system, can solve the problems of limiting the very early diagnosis of gastric cancer, the long transformation time of gastritis cancer, limited sensitivity and specificity, etc.

Inactive Publication Date: 2020-10-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the diagnosis of early gastric cancer is still based on imaging, endoscopy, and histopathology, all of which have certain limitations.
Diagnosis based on imaging cannot accurately observe changes at the cellular level, and key biological features are easily missed; while diagnostic methods based on histopathological observation and conventional tumor marker molecular expression are limited by sensitivity and specificity
For example, the classic gastric cancer marker CEACAM5 is highly expressed in gastric cancer cells and other types of adjacent cells (such as intestinal cells and normal gastric epithelial cells), so it is difficult to be a specific marker for early screening of gastric cancer
More importantly, due to the long time of gastritis cancer transformation, it is difficult to define the key transformation point of gastritis cancer transformation, that is, the "cancer initiation point". key bottleneck

Method used

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  • Gastric cancer extremely-early cell marker, gastric cancer precancerous lesion early cell marker and application of markers in diagnostic kits
  • Gastric cancer extremely-early cell marker, gastric cancer precancerous lesion early cell marker and application of markers in diagnostic kits
  • Gastric cancer extremely-early cell marker, gastric cancer precancerous lesion early cell marker and application of markers in diagnostic kits

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Experimental program
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Effect test

Embodiment 1

[0052] In order to verify the role of the present invention in assisting early diagnosis of gastric precancerous lesions and digestive system tumors, and predicting the risk of gastric cancer recurrence, the inventors independently analyzed gastric precancerous lesions, gastric early cancer, gastric cancer recurrence, and other digestive system tumors (colon cancer, esophageal cancer, etc.) Carcinoma and pancreatic cancer) and typical cases of precancerous lesions were analyzed.

[0053] Diagnosis of very early stage cells in gastric precancerous lesions

[0054] First, the inventors evaluated the expression of HES6, a very early cell marker of gastric precancerous lesions, in different types of cells in independent gastric precancerous lesions samples. The result is as Figure 2A As shown, HES6 is specifically highly expressed in gastric precancerous lesion cells.

[0055] Secondly, the inventors evaluated the co-expression of the very early cell marker HES6 of gastric prec...

Embodiment 2

[0103] The present inventor obtains five representative components (KLK10, KRT17, TIMP1, MAP17 and KLK6) in the normal Expression in gastric tissue, gastric precancerous lesion, early cancer and advanced cancer. The kit utilizes immunohistochemical staining (IHC) to measure marker expression levels. 10% buffered formalin was used to fix paraffin-embedded surgical samples, and the tissue section was 4 μm / sheet.

[0104] The test kit in this embodiment includes the following components:

[0105] (1) Reagent A: blocking solution, which is 10% goat serum;

[0106] (2) Reagent B: Diluted ready-to-use anti-Klk10 primary antibody (it can also be any one of KRT17, TIMP1, MAP17 and KLK6 antibodies in Table 1);

[0107] (4) Reagent C: anti-goat biotinylated secondary antibody;

[0108] (5) Reagent D: streptavidin-labeled HRP;

[0109] (6) Reagent E: 20 times concentrated DAB substrate solution;

[0110] (7) Reagent F: 20 times concentrated DAB substrate buffer solution;

[0111] ...

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Abstract

The inventor finds out a cell population (named as gastric cancer very early cells) which starts to appear in the low-grade heteroplasmal hyperplasia stage of the stomach, has specific molecular characteristics and has a high canceration risk, and the cell population can be used as a marker for very early diagnosis of gastric cancer. Molecular markers of gastric cancer extremely-early cells are applied to preparation of a kit for realizing early diagnosis of gastric cancer or other digestive system tumors based on gastric tissues or blood samples. The gastric cancer extremely-early cell markercan also distinguish the postoperative recurrence risk of gastric cancer and the tumor risk of digestive system organs such as intestines, pancreas and esophagus. Besides, the inventor also finds outa cell population which appears in the early stage of gastric precancerous lesions and has specific molecular characteristics, and the cell population can be used as a marker for early diagnosis of gastric precancerous lesions and is also integrated into the kit. The markers and kits can be used for early clinical diagnosis of gastric cancer and other digestive tract tumors, can also be used as an intervention target for prevention and treatment of the digestive tract tumors, and have a good application prospect.

Description

technical field [0001] The present invention relates to a cell marker and its measurement system and method for assisting very early diagnosis of gastric cancer and other digestive system tumors, and also relates to a cell marker for early diagnosis of gastric precancerous lesions and its measurement system and method. Background technique [0002] Gastric cancer is a common malignant tumor of the digestive system with a high mortality rate. The occurrence of most gastric cancers will go through a series of precancerous lesions, including intestinal metaplasia and dysplasia. The risk of developing gastric cancer in patients with precancerous lesions is nearly three times that of patients without precancerous lesions [1]. Early diagnosis of gastric precancerous lesions is of great significance for the prevention of gastric cancer. [0003] In addition to gastric precancerous lesions, the very early diagnosis of gastric cancer is also of great significance for the prevention...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57446G01N33/57484G01N2333/47G01N33/574
Inventor 李梢张鹏
Owner TSINGHUA UNIV
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