A kind of bacillus subtilis bs40-4 and its application

A technology of Bacillus subtilis and bacteria powder, which is applied to bacteria, fixed on or in inorganic carriers, fixed on/in organic carriers, etc., can solve the problem of low decomposing temperature, poor degradation efficiency, secondary pollution, etc. question

Active Publication Date: 2021-04-09
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of traditional aerobic microorganisms in decomposing organic wastes such as livestock and poultry manure with complex components, such as long decomposing period, low decomposing temperature, poor degradation efficiency and easy secondary pollution, the present invention provides a subtilis spore Bacillus BS40-4 and its application

Method used

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  • A kind of bacillus subtilis bs40-4 and its application
  • A kind of bacillus subtilis bs40-4 and its application
  • A kind of bacillus subtilis bs40-4 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] 1.1 Isolation of strains

[0034] Cow manure-straw composting product samples were collected from the Dairy Farm in Shijiazhuang City, Hebei Province, and separated by LB medium (formula: tryptone 10g / L, yeast extract 5g / L, NaCl10g / L) by dilution and plating, 45 strains were obtained, and after five plate passages, stable genetic monoclonal strains were obtained and stored at -80°C.

[0035] The isolated 45 monoclonal strains were treated with cellulose Congo red medium (recipe: sodium nitrate 1.0g, disodium hydrogen phosphate 1.2g, potassium dihydrogen phosphate 0.9g, magnesium sulfate 0.5g, potassium chloride 0.5g, yeast leaching Powder 0.5g, acid hydrolyzed casein 0.5g, Congo red 0.2g, cellulose powder 5.0g, agar 15.0g, pH 7.0±0.1, 1000ml distilled water, autoclaved at 121°C for 15min), cultivated in a 50°C incubator for 72h According to the transparent circle index of the culture medium colony (diameter of transparent circle D / colony diameter d≥4), isolate and scre...

Embodiment 2

[0063] Preparation of solid microbial agent of Bacillus subtilis BS40-4

[0064] The Bacillus subtilis BS40-4 preserved in embodiment 1 is carried out successively:

[0065] Strain activation: Streak inoculation of BS40-4 strain stored at -80°C on LB plates, and culture at 50°C for 24 hours.

[0066] Primary seed culture: Scrape off the BS40-4 colony on the LB plate with an inoculation loop and inoculate it in a Erlenmeyer flask filled with LB liquid medium, and cultivate it on a shaker at 50°C and 200r / min for 24h.

[0067] Secondary seed fermentation: Inoculate the above-mentioned primary seed culture liquid into a seed tank containing 10 L of LB liquid medium at an inoculation amount of 10%, and ferment for 1 day at 50° C. and 200 rpm / min.

[0068] Expanded cultivation: the obtained secondary seed fermentation liquid was inoculated into a fermenter equipped with 50 L of LB liquid medium at an inoculum size of 5%, and fermented at 50° C. and 200 rpm / min for 2 days.

[0069...

Embodiment 3

[0072] Preparation of liquid bacterial agent of Bacillus subtilis BS40-4

[0073] The expanded culture solution of Bacillus subtilis BS40-4 obtained in Example 2 is obtained by the method of plate frame filtration to obtain Bacillus subtilis BS40-4 thalline.

[0074] The obtained Bacillus subtilis BS40-4 thalline is added in the nutrient solution (containing mass concentration is the inorganic nutrient of 10-20%, in the inorganic nutrient, N, P 2 o 5 and K 2 The mass ratio of O is 3:1:1), so that the number of viable bacteria in it reaches 1×10 9 CFU / mL, to obtain Bacillus subtilis BS40-4 liquid inoculum.

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Abstract

The invention relates to the technical field of environmental microorganisms, and specifically discloses a Bacillus subtilis BS40‑4 and an application thereof. The strain preservation number of the Bacillus subtilis BS40-4 is CGMCC No.19757. The strain has extreme high-temperature activity, can maintain growth at a high temperature of 110°C, and has a wide range of suitable growth temperatures, can maintain high activity at 30-100°C, and has strong resistance to external harmful factors (high temperature, strong acid, Alkali, etc.) or bad stimulation ability, can survive in a wide pH (4.0-12.0) environment and maintain high activity, so it can be applied to the decomposition and fermentation of organic waste.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a bacillus subtilis BS40-4 and an application thereof. Background technique [0002] With the increasing production scale of my country's livestock and poultry breeding industry, livestock and poultry manure has become one of the factors of environmental pollution. Therefore, the efficient treatment of livestock and poultry manure has become the key to solving the problem of organic waste pollution. The high-temperature composting process is one of the important ways to recycle livestock manure. Its mechanism is to use the metabolism of microorganisms under suitable conditions to realize the oxidation, mineralization and aromatization of organic matter. Livestock and poultry manure species contain a large amount of macromolecular lignocellulose that is difficult to degrade. Therefore, the traditional process of composting livestock and poultry manure pays mor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/14C05F17/20C12R1/125
CPCC12N1/20C12N11/10C12N11/14C05F17/20C12R2001/125C12N1/205Y02W30/40Y02P20/145
Inventor 李再兴马骏武肖莎黄亚丽韩学滨秦学刘双
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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